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The map label for this gene is vipB [H]

Identifier: 82701405

GI number: 82701405

Start: 292552

End: 293604

Strand: Direct

Name: vipB [H]

Synonym: Nmul_A0271

Alternate gene names: 82701405

Gene position: 292552-293604 (Clockwise)

Preceding gene: 82701404

Following gene: 82701410

Centisome position: 9.19

GC content: 52.8

Gene sequence:

>1053_bases
ATGGCTGCTTACCCAAAATGGCTGCGGCATTTGCAGGACCATTCCTACTGCTGGCTGGTCACGGGGGTTGCGGGCTTCAT
CGGATCCAATCTGCTGGAGGCCTTGCTCAAGCATAATCAGAAAGTAGTCGGATTGGATAATTTTTCCACCGGCTATTTGC
GAAACCTCGAGCAGATACGGGACTTGGTCGGGGAGAAGGCTTGGGGTAATTTCAGCTTTATAGAGGGTGATATCTGTCAA
CTGGAAACTTGCACGAACGCATGCCAAGGTGTGGATTTCGTTTTGCACCAGGCCGCGCTGGGTTCCGTGCCGCGTTCCAT
TCAAGATCCCATCCGAACCAACGAAGCCAATATTTCCGGATTTCTCAACATGCTGGTGGCGTCAAGGGATGCGCAAGTGA
GGCGCTTCATCTACGCTGCCTCCAGTTCCACCTACGGCGACCACCCGGATTTGCCAAAAGTGGAAGCGGTAATTGGACGT
CCTCTTTCGCCCTATGCTGTCACCAAATATGTGAACGAACTCTACGCCGAAGTGTTCGCGCGTTGCTATGGACTGGACTC
CATTGGGCTGCGCTATTTCAACGTATTCGGTCCAAGGCAGGATCCCAATGGTGCTTATGCCGCAGTTATTCCCCAATGGG
TTTCGGCACTGATCAGAAACCAGACGCTGTATATCAATGGGGATGGGGAAACCAGCCGGGATTTCTGTTATATCGACAAC
GTAGTGCAAGCCAATCTTCTCGCGGCTCTCAGTGATAACACCGGAGCAGTGAACCAGATTTACAATGTGGCAGTAAATGA
ACGCACCAGTCTGAATCAACTGTATGGCATGATGCGCGAGCTGTTACTGGAGAAGTTTCCGGAGCTGGAGAATCATCGGC
CCACGTACGTCGATTTTCGCAAGGGCGATGTGCGGCATTCACAGGCGGATATTACGAAAGCCACCCAATTACTTGGTTTT
GAACCCTCCCACCGCATCGGGGAAGGACTGAGGCAGGCAATGGGCTGGTACATCGCGCATTTGGGGGCTATGCAGGAGGC
GGCTGGCGTCTAA

Upstream 100 bases:

>100_bases
ACAAAGGGTGTTTCATCGATGTGAAATCCCAGTTCGATCCTGCCGCGCTGCGAGAGGCTGGCTTAAACGTGTGGAGACTG
TGAAGGATAGTACGCCTGGT

Downstream 100 bases:

>100_bases
ACGTTCGTCGGAACTTCGATTCGCGCTAATCCCCGCTTTCCGCGCTATTCATCCCATTTTCCCGCTTGTTTCAATTGATT
CTTCAAGCCCGGTAACGGAA

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAAYPKWLRHLQDHSYCWLVTGVAGFIGSNLLEALLKHNQKVVGLDNFSTGYLRNLEQIRDLVGEKAWGNFSFIEGDICQ
LETCTNACQGVDFVLHQAALGSVPRSIQDPIRTNEANISGFLNMLVASRDAQVRRFIYAASSSTYGDHPDLPKVEAVIGR
PLSPYAVTKYVNELYAEVFARCYGLDSIGLRYFNVFGPRQDPNGAYAAVIPQWVSALIRNQTLYINGDGETSRDFCYIDN
VVQANLLAALSDNTGAVNQIYNVAVNERTSLNQLYGMMRELLLEKFPELENHRPTYVDFRKGDVRHSQADITKATQLLGF
EPSHRIGEGLRQAMGWYIAHLGAMQEAAGV

Sequences:

>Translated_350_residues
MAAYPKWLRHLQDHSYCWLVTGVAGFIGSNLLEALLKHNQKVVGLDNFSTGYLRNLEQIRDLVGEKAWGNFSFIEGDICQ
LETCTNACQGVDFVLHQAALGSVPRSIQDPIRTNEANISGFLNMLVASRDAQVRRFIYAASSSTYGDHPDLPKVEAVIGR
PLSPYAVTKYVNELYAEVFARCYGLDSIGLRYFNVFGPRQDPNGAYAAVIPQWVSALIRNQTLYINGDGETSRDFCYIDN
VVQANLLAALSDNTGAVNQIYNVAVNERTSLNQLYGMMRELLLEKFPELENHRPTYVDFRKGDVRHSQADITKATQLLGF
EPSHRIGEGLRQAMGWYIAHLGAMQEAAGV
>Mature_349_residues
AAYPKWLRHLQDHSYCWLVTGVAGFIGSNLLEALLKHNQKVVGLDNFSTGYLRNLEQIRDLVGEKAWGNFSFIEGDICQL
ETCTNACQGVDFVLHQAALGSVPRSIQDPIRTNEANISGFLNMLVASRDAQVRRFIYAASSSTYGDHPDLPKVEAVIGRP
LSPYAVTKYVNELYAEVFARCYGLDSIGLRYFNVFGPRQDPNGAYAAVIPQWVSALIRNQTLYINGDGETSRDFCYIDNV
VQANLLAALSDNTGAVNQIYNVAVNERTSLNQLYGMMRELLLEKFPELENHRPTYVDFRKGDVRHSQADITKATQLLGFE
PSHRIGEGLRQAMGWYIAHLGAMQEAAGV

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=30.1204819277108, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=28.3536585365854, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI56237023, Length=328, Percent_Identity=26.5243902439024, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI56118217, Length=328, Percent_Identity=26.5243902439024, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI189083684, Length=328, Percent_Identity=26.5243902439024, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=29.3948126801153, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786974, Length=344, Percent_Identity=28.4883720930233, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=28.735632183908, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790049, Length=320, Percent_Identity=24.0625, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=26.4880952380952, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=25, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=348, Percent_Identity=26.4367816091954, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=350, Percent_Identity=26.2857142857143, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115532424, Length=335, Percent_Identity=24.4776119402985, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=348, Percent_Identity=25, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356223, Length=326, Percent_Identity=28.2208588957055, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19923002, Length=348, Percent_Identity=27.8735632183908, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39034; Mature: 38902

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYPKWLRHLQDHSYCWLVTGVAGFIGSNLLEALLKHNQKVVGLDNFSTGYLRNLEQIR
CCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
DLVGEKAWGNFSFIEGDICQLETCTNACQGVDFVLHQAALGSVPRSIQDPIRTNEANISG
HHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHCCCCHHHH
FLNMLVASRDAQVRRFIYAASSSTYGDHPDLPKVEAVIGRPLSPYAVTKYVNELYAEVFA
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RCYGLDSIGLRYFNVFGPRQDPNGAYAAVIPQWVSALIRNQTLYINGDGETSRDFCYIDN
HHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEHHHH
VVQANLLAALSDNTGAVNQIYNVAVNERTSLNQLYGMMRELLLEKFPELENHRPTYVDFR
HHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
KGDVRHSQADITKATQLLGFEPSHRIGEGLRQAMGWYIAHLGAMQEAAGV
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAYPKWLRHLQDHSYCWLVTGVAGFIGSNLLEALLKHNQKVVGLDNFSTGYLRNLEQIR
CCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
DLVGEKAWGNFSFIEGDICQLETCTNACQGVDFVLHQAALGSVPRSIQDPIRTNEANISG
HHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHCCCCHHHH
FLNMLVASRDAQVRRFIYAASSSTYGDHPDLPKVEAVIGRPLSPYAVTKYVNELYAEVFA
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RCYGLDSIGLRYFNVFGPRQDPNGAYAAVIPQWVSALIRNQTLYINGDGETSRDFCYIDN
HHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEHHHH
VVQANLLAALSDNTGAVNQIYNVAVNERTSLNQLYGMMRELLLEKFPELENHRPTYVDFR
HHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
KGDVRHSQADITKATQLLGFEPSHRIGEGLRQAMGWYIAHLGAMQEAAGV
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]