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The map label for this gene is hcaE [H]

Identifier: 82701367

GI number: 82701367

Start: 247736

End: 248833

Strand: Reverse

Name: hcaE [H]

Synonym: Nmul_A0233

Alternate gene names: 82701367

Gene position: 248833-247736 (Counterclockwise)

Preceding gene: 82701379

Following gene: 82701366

Centisome position: 7.81

GC content: 53.1

Gene sequence:

>1098_bases
ATGGTCGATCTGATTGATGTTTGCCAATTGGCTCCAACGCAACTGCCGGTTGACTGGTATCTCGACTCTCAAATCCTTGA
ACTGGAAAAACGCATTCTTTTCGACCAGGGACCGGGCTATGTCGGTCATGAGATCATGGTGCCCAACATTGGTGACTATT
ATGTGCCCGAATGGATGGATAACGCGAAAATGCTGGTGCGTAATAAGGATGGTATCGAGCTGCTGTCCAACGTCTGCCGC
CACAGGCAGTCACTGCTGCTCAAAGGGAGTGGCAACACCAGAAACATCGTTTGCCCGGTGCATCGCTGGACGTATGACTT
GAAGGGCACGTTGCTCGGGGCGCCCCATTTCCCCGAAAACCCTTGCCTCAATCTTTCCAATTCACCTTTGCAGAACTGGA
ACGGACTCCTGTTTCAAGGCAGGCGGAATGTTGCCCGCGATCTGGGCAACTTGCAAGTCCTGAAGAATTTCGACTTCTCG
GGCCATGTGCTGGAACGCCTCCAGATAGATGAATACGCCTGCAACTGGAAAACCTTCATAGAAGTTTATCTGGAGGACTA
CCACGTCGAGCCATACCATCCGGGCCTGGGCAACTTTGTTGACACGGCTGCGCTGGAATGGGAGTTCGGGGAGTGGTACA
ACGTGCAGACTGTGGGGATCAATAACGCCCTGACCCGTCCGGGAACGCCCGTCTATGCAAAATGGCACGAGCAACTGTTG
CTGCAGACAGCGGGCGAAATACCGAGGCATGGCGCAATCTGGATGCTGTATTATCCCAATGTCATGATGGAATGGTATCC
CCATGTATTGGTCGTCAGCACGGTGCTTCCTACCGGAACAGAGCGTTGCACCAATGTGGTGGAGTTCTATTATCCTGAAG
ATATCGCTTTGTTTGAGCGTGAATTCATTGAAGCAGAACAGGCCGCCTATCGCGAAACCGCTGCCGAGGATGATGAAATC
TGCAGGCTGATGACGGAAGGGCGCCGTGCGCTGTACAAACAAGGCGTGAGCGAGGTCGGACCGTACCAATCGCCGATGGA
AGATGGCATGGTGCACTTCCACAAGTTTCTGCGGCGAGAAATTGAACCGCACATCTGA

Upstream 100 bases:

>100_bases
CATGAGAGCAGGTGAGCATGCTCATTCTCTTCCTGATCTTTCCTGCCGTTCCTGGTTTTGATAATCCAGCCATCTTTATT
TGAAAGGTGAAAAGTAGCAT

Downstream 100 bases:

>100_bases
GCAATAAGTGATATCCTCTTGCTGGGCGGATTTTTCTTCGATAGCCCGAATTTTTTGGACGGATTTTTCTCTGGCCGCGC
AATTTTATATTGTGGGTAAG

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MVDLIDVCQLAPTQLPVDWYLDSQILELEKRILFDQGPGYVGHEIMVPNIGDYYVPEWMDNAKMLVRNKDGIELLSNVCR
HRQSLLLKGSGNTRNIVCPVHRWTYDLKGTLLGAPHFPENPCLNLSNSPLQNWNGLLFQGRRNVARDLGNLQVLKNFDFS
GHVLERLQIDEYACNWKTFIEVYLEDYHVEPYHPGLGNFVDTAALEWEFGEWYNVQTVGINNALTRPGTPVYAKWHEQLL
LQTAGEIPRHGAIWMLYYPNVMMEWYPHVLVVSTVLPTGTERCTNVVEFYYPEDIALFEREFIEAEQAAYRETAAEDDEI
CRLMTEGRRALYKQGVSEVGPYQSPMEDGMVHFHKFLRREIEPHI

Sequences:

>Translated_365_residues
MVDLIDVCQLAPTQLPVDWYLDSQILELEKRILFDQGPGYVGHEIMVPNIGDYYVPEWMDNAKMLVRNKDGIELLSNVCR
HRQSLLLKGSGNTRNIVCPVHRWTYDLKGTLLGAPHFPENPCLNLSNSPLQNWNGLLFQGRRNVARDLGNLQVLKNFDFS
GHVLERLQIDEYACNWKTFIEVYLEDYHVEPYHPGLGNFVDTAALEWEFGEWYNVQTVGINNALTRPGTPVYAKWHEQLL
LQTAGEIPRHGAIWMLYYPNVMMEWYPHVLVVSTVLPTGTERCTNVVEFYYPEDIALFEREFIEAEQAAYRETAAEDDEI
CRLMTEGRRALYKQGVSEVGPYQSPMEDGMVHFHKFLRREIEPHI
>Mature_365_residues
MVDLIDVCQLAPTQLPVDWYLDSQILELEKRILFDQGPGYVGHEIMVPNIGDYYVPEWMDNAKMLVRNKDGIELLSNVCR
HRQSLLLKGSGNTRNIVCPVHRWTYDLKGTLLGAPHFPENPCLNLSNSPLQNWNGLLFQGRRNVARDLGNLQVLKNFDFS
GHVLERLQIDEYACNWKTFIEVYLEDYHVEPYHPGLGNFVDTAALEWEFGEWYNVQTVGINNALTRPGTPVYAKWHEQLL
LQTAGEIPRHGAIWMLYYPNVMMEWYPHVLVVSTVLPTGTERCTNVVEFYYPEDIALFEREFIEAEQAAYRETAAEDDEI
CRLMTEGRRALYKQGVSEVGPYQSPMEDGMVHFHKFLRREIEPHI

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788103, Length=375, Percent_Identity=24.2666666666667, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788888, Length=190, Percent_Identity=30, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 42294; Mature: 42294

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDLIDVCQLAPTQLPVDWYLDSQILELEKRILFDQGPGYVGHEIMVPNIGDYYVPEWMD
CCCHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHC
NAKMLVRNKDGIELLSNVCRHRQSLLLKGSGNTRNIVCPVHRWTYDLKGTLLGAPHFPEN
CCEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCEEEECHHHEEEEECEEEEECCCCCCC
PCLNLSNSPLQNWNGLLFQGRRNVARDLGNLQVLKNFDFSGHVLERLQIDEYACNWKTFI
CCCCCCCCCCCCCCCEEEECCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHH
EVYLEDYHVEPYHPGLGNFVDTAALEWEFGEWYNVQTVGINNALTRPGTPVYAKWHEQLL
HHHHHHCCCCCCCCCCCCHHHHHEEEEECCCEEEEEEEECCCCCCCCCCCCHHHHHHHHH
LQTAGEIPRHGAIWMLYYPNVMMEWYPHVLVVSTVLPTGTERCTNVVEFYYPEDIALFER
HHHHCCCCCCCCEEEEECCCHHHHHCCHHEEEEHHCCCCHHHHHHHHHEECCCHHHHHHH
EFIEAEQAAYRETAAEDDEICRLMTEGRRALYKQGVSEVGPYQSPMEDGMVHFHKFLRRE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHH
IEPHI
CCCCC
>Mature Secondary Structure
MVDLIDVCQLAPTQLPVDWYLDSQILELEKRILFDQGPGYVGHEIMVPNIGDYYVPEWMD
CCCHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHC
NAKMLVRNKDGIELLSNVCRHRQSLLLKGSGNTRNIVCPVHRWTYDLKGTLLGAPHFPEN
CCEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCEEEECHHHEEEEECEEEEECCCCCCC
PCLNLSNSPLQNWNGLLFQGRRNVARDLGNLQVLKNFDFSGHVLERLQIDEYACNWKTFI
CCCCCCCCCCCCCCCEEEECCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHH
EVYLEDYHVEPYHPGLGNFVDTAALEWEFGEWYNVQTVGINNALTRPGTPVYAKWHEQLL
HHHHHHCCCCCCCCCCCCHHHHHEEEEECCCEEEEEEEECCCCCCCCCCCCHHHHHHHHH
LQTAGEIPRHGAIWMLYYPNVMMEWYPHVLVVSTVLPTGTERCTNVVEFYYPEDIALFER
HHHHCCCCCCCCEEEEECCCHHHHHCCHHEEEEHHCCCCHHHHHHHHHEECCCHHHHHHH
EFIEAEQAAYRETAAEDDEICRLMTEGRRALYKQGVSEVGPYQSPMEDGMVHFHKFLRRE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHH
IEPHI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]