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The map label for this gene is lpsE [H]

Identifier: 82701304

GI number: 82701304

Start: 177163

End: 178338

Strand: Direct

Name: lpsE [H]

Synonym: Nmul_A0170

Alternate gene names: 82701304

Gene position: 177163-178338 (Clockwise)

Preceding gene: 82701301

Following gene: 82701306

Centisome position: 5.56

GC content: 49.91

Gene sequence:

>1176_bases
ATGATAAAAAAAGAAAAGCACAAGACAATCATAATGCTCGGAACATCGCCTGAAGGACATGGCGGAATCGCGACTGTGGT
CGGCAACTACGGAGATTGCGGTGTCCTGAAGAAATGGAAGGTCAAATATGTAGTGACGCATGTGCAGGGCTCGGGATTTG
CAAAAGCATGTGCGGCAGGAGTAGCGATGCAACAGTTACTCTGCATGCTGGTTACTGGCCGGATCGGATTGCTTCATGTG
CATATGTCCTCAAGGGCAAGCACATGGAGAAAGTCACTTTTTCTGCTGATGGGGATGGTGTTTCGGGTCCCCTATCTCAT
TCACTTGCACAGCCCGAATTTCGTTGATTTTTTTGAGCATGAGTGCGGAGAACGTAGAAAGAGGTTAATCAGATTTCTGC
TATCGCGGGCGCTATATGTTATTGCCTTATCTCAAGGTTGGGCTAAAGATATTAAGAAGATTTCTCCTGCTGCACGTACA
GTAATTCTTTTTAACTCCGTACCGCTACCTGCTGCGAGGTTGCGCGAGAAAGAGATGGAAGAAAGTAGCAACGCGCTATG
TGATCCTCCTCTTATATTATTTCTTGGTCATGTCGGTAAGCGTAAAGGTACATTTGACCTGATCCGAGCGGTAGCGTTAC
TGAGCGAAAATTTCAGGTTAATTATCGGAGGCGACGGCGAACTCCAGCGGGCGCAGATGTTGTCGGAAGAGCTGGGTGTC
AGTGACAAGATTCTGTTTGCGGGGTGGCTTGGCAAAGCAGAAAAAGATCATTTGCTCGCTCGAGCAGCTATCTTTGTACT
TCCTTCGTACCACGAAGGGGTGCCTATGGCGATCCTGGAAGCAATGTCCTGGGGAATTCCCATTGTAACCACCCCTGTGG
GGGGCATACCCGAGGTGGTAACGGAAGGACAAGAGGGCCTACTGGTAAATTCCGGTGATATCGTAGGACTCGCACATGCG
TTAGCGCGTCTGCTTGCCGCACCATCCCTTCGCCGGGAATTCGGAGAAAGAGGAAGGCACAAGATCGAGTCCAAGTTCTC
TATAAAGGTTCTGCAACCGCAATTGGAACAGTTGTGGATAGACTCGGGTGTATCAGAGCCAGAGCCAAGGCCAGAGCCAG
AGCTAGTGATGCAGAGAGGGGAGGTGCCTGCCCCCGAGATTCAGCCTAAAGTATGA

Upstream 100 bases:

>100_bases
ATATCAATAGGCATTCAGCCAGCCCATGCGCCCATGGATTCCTGCCACTAGAAAATGCCTATGAGCCTGTTTGTTCCCTT
CCCGTTGAGTTGAATCGATT

Downstream 100 bases:

>100_bases
TCACCGCCTACTCCATTACCGCCGGCAACTCGGCAGCCAGAGCATTCTTTTCCTTGGTTGACGCTCCCAGCAGGGCATAG
CCGAGCAGTTTTCCCTCGGC

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIKKEKHKTIIMLGTSPEGHGGIATVVGNYGDCGVLKKWKVKYVVTHVQGSGFAKACAAGVAMQQLLCMLVTGRIGLLHV
HMSSRASTWRKSLFLLMGMVFRVPYLIHLHSPNFVDFFEHECGERRKRLIRFLLSRALYVIALSQGWAKDIKKISPAART
VILFNSVPLPAARLREKEMEESSNALCDPPLILFLGHVGKRKGTFDLIRAVALLSENFRLIIGGDGELQRAQMLSEELGV
SDKILFAGWLGKAEKDHLLARAAIFVLPSYHEGVPMAILEAMSWGIPIVTTPVGGIPEVVTEGQEGLLVNSGDIVGLAHA
LARLLAAPSLRREFGERGRHKIESKFSIKVLQPQLEQLWIDSGVSEPEPRPEPELVMQRGEVPAPEIQPKV

Sequences:

>Translated_391_residues
MIKKEKHKTIIMLGTSPEGHGGIATVVGNYGDCGVLKKWKVKYVVTHVQGSGFAKACAAGVAMQQLLCMLVTGRIGLLHV
HMSSRASTWRKSLFLLMGMVFRVPYLIHLHSPNFVDFFEHECGERRKRLIRFLLSRALYVIALSQGWAKDIKKISPAART
VILFNSVPLPAARLREKEMEESSNALCDPPLILFLGHVGKRKGTFDLIRAVALLSENFRLIIGGDGELQRAQMLSEELGV
SDKILFAGWLGKAEKDHLLARAAIFVLPSYHEGVPMAILEAMSWGIPIVTTPVGGIPEVVTEGQEGLLVNSGDIVGLAHA
LARLLAAPSLRREFGERGRHKIESKFSIKVLQPQLEQLWIDSGVSEPEPRPEPELVMQRGEVPAPEIQPKV
>Mature_391_residues
MIKKEKHKTIIMLGTSPEGHGGIATVVGNYGDCGVLKKWKVKYVVTHVQGSGFAKACAAGVAMQQLLCMLVTGRIGLLHV
HMSSRASTWRKSLFLLMGMVFRVPYLIHLHSPNFVDFFEHECGERRKRLIRFLLSRALYVIALSQGWAKDIKKISPAART
VILFNSVPLPAARLREKEMEESSNALCDPPLILFLGHVGKRKGTFDLIRAVALLSENFRLIIGGDGELQRAQMLSEELGV
SDKILFAGWLGKAEKDHLLARAAIFVLPSYHEGVPMAILEAMSWGIPIVTTPVGGIPEVVTEGQEGLLVNSGDIVGLAHA
LARLLAAPSLRREFGERGRHKIESKFSIKVLQPQLEQLWIDSGVSEPEPRPEPELVMQRGEVPAPEIQPKV

Specific function: Adds A Galactose Goup To A Glucose Group Of Lps. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI226510984, Length=202, Percent_Identity=26.2376237623762, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788357, Length=242, Percent_Identity=28.9256198347107, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790061, Length=160, Percent_Identity=28.125, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17532709, Length=122, Percent_Identity=33.6065573770492, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 43116; Mature: 43116

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKEKHKTIIMLGTSPEGHGGIATVVGNYGDCGVLKKWKVKYVVTHVQGSGFAKACAAG
CCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHCEEEEEEEEECCCCHHHHHHHH
VAMQQLLCMLVTGRIGLLHVHMSSRASTWRKSLFLLMGMVFRVPYLIHLHSPNFVDFFEH
HHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
ECGERRKRLIRFLLSRALYVIALSQGWAKDIKKISPAARTVILFNSVPLPAARLREKEME
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHH
ESSNALCDPPLILFLGHVGKRKGTFDLIRAVALLSENFRLIIGGDGELQRAQMLSEELGV
HHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCC
SDKILFAGWLGKAEKDHLLARAAIFVLPSYHEGVPMAILEAMSWGIPIVTTPVGGIPEVV
CCEEEEEECCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHH
TEGQEGLLVNSGDIVGLAHALARLLAAPSLRREFGERGRHKIESKFSIKVLQPQLEQLWI
HCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
DSGVSEPEPRPEPELVMQRGEVPAPEIQPKV
HCCCCCCCCCCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIKKEKHKTIIMLGTSPEGHGGIATVVGNYGDCGVLKKWKVKYVVTHVQGSGFAKACAAG
CCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHCEEEEEEEEECCCCHHHHHHHH
VAMQQLLCMLVTGRIGLLHVHMSSRASTWRKSLFLLMGMVFRVPYLIHLHSPNFVDFFEH
HHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
ECGERRKRLIRFLLSRALYVIALSQGWAKDIKKISPAARTVILFNSVPLPAARLREKEME
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHH
ESSNALCDPPLILFLGHVGKRKGTFDLIRAVALLSENFRLIIGGDGELQRAQMLSEELGV
HHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCC
SDKILFAGWLGKAEKDHLLARAAIFVLPSYHEGVPMAILEAMSWGIPIVTTPVGGIPEVV
CCEEEEEECCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHH
TEGQEGLLVNSGDIVGLAHALARLLAAPSLRREFGERGRHKIESKFSIKVLQPQLEQLWI
HCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
DSGVSEPEPRPEPELVMQRGEVPAPEIQPKV
HCCCCCCCCCCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157937; 11481430 [H]