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The map label for this gene is insD1 [C]

Identifier: 82701302

GI number: 82701302

Start: 175290

End: 176138

Strand: Reverse

Name: insD1 [C]

Synonym: Nmul_A0168

Alternate gene names: 82701302

Gene position: 176138-175290 (Counterclockwise)

Preceding gene: 82701303

Following gene: 82701300

Centisome position: 5.53

GC content: 53.95

Gene sequence:

>849_bases
ATGAAGGCCATTGCAGACACCATGGGTGTGGCGCGCTCCCGTCTTGTGGAACGGATGAAGCCGCAGGAAAAGTCTTTTCG
TTGCCGCTACAACAAGGCTGATGATGCCTGGCTGTTGCCCTTGATCCGCGAAATTGTGGATTGCCGCGCGACCTACGGAT
ACAGAAGGATTTGCGCTCTCCTTAACCGCAAACTGGAGAGAATGGGCAAGCCTGCTGTCAATCACAAGCGGGTCTATCGC
ATCATGCGCCAGAATGGCCTGCTGCTGGCCCGATATACGGGCAAACAGCGCGTGCTGTCTCATGAAGGCAAGGTCATTAC
CTTGCGTTCCAATTTGCGCTGGTGTTCGGATGGTTTTGAGATTCCCTGCTGGAATGGGCAAGTAGTGCGTGTTGCCTTCG
CCCTGGATTGCTGTGATCGTGAAGTCATCAGCCATGTTGCCACCACTGGCGGCATTACTGGGGAAATGGTGCGCGATCTG
ATGACTGAATCCGTTGAGCGGCGCTTCGGCACCGTTGACCTGCTCCCACACAGGGTGGAGTGGCTCAGCGACAATGGCAG
CTGTTACACCGCCAGCGAGACAACTGCTTTTGCAAAGGATATGGGCTTCATCAGCTGTTTTACTCCCGTCAGAAGTCCCG
AATCCAACGGTATGGCCGAGGCCTTCGTCAAAACCTTCAAGCGGGATTACGTCTATGTCCATGATCGTCCCGACGCAAAA
ACCGTAATGGCGCAGCTTGACCAATGGTTCGAGGATTACAATGAATTCCATCCTCACAAGGGGCTGAAGATGAAGTCGCC
AAGACAATTCATCCGCAGCCAGTTACTAACCGCATCCTGTCCGGTTTAA

Upstream 100 bases:

>100_bases
AGAAAACCCTGGAGAACGAGATTCTCACGGAAGCGGTGAAGCTTGCGCATGAAAAAAAACTGATCTCGCGCATGCCCTTG
CTGCCACCGGACGATTTGCC

Downstream 100 bases:

>100_bases
TAGGGGCAACTCCATTTAGTAAGGTCAAGATAAACTGCTCATACTCTGTTCCGATCTACTTCATTACATCTCCCATCTTT
GGGGTTTACCGAGCATGAAA

Product: integrase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKAIADTMGVARSRLVERMKPQEKSFRCRYNKADDAWLLPLIREIVDCRATYGYRRICALLNRKLERMGKPAVNHKRVYR
IMRQNGLLLARYTGKQRVLSHEGKVITLRSNLRWCSDGFEIPCWNGQVVRVAFALDCCDREVISHVATTGGITGEMVRDL
MTESVERRFGTVDLLPHRVEWLSDNGSCYTASETTAFAKDMGFISCFTPVRSPESNGMAEAFVKTFKRDYVYVHDRPDAK
TVMAQLDQWFEDYNEFHPHKGLKMKSPRQFIRSQLLTASCPV

Sequences:

>Translated_282_residues
MKAIADTMGVARSRLVERMKPQEKSFRCRYNKADDAWLLPLIREIVDCRATYGYRRICALLNRKLERMGKPAVNHKRVYR
IMRQNGLLLARYTGKQRVLSHEGKVITLRSNLRWCSDGFEIPCWNGQVVRVAFALDCCDREVISHVATTGGITGEMVRDL
MTESVERRFGTVDLLPHRVEWLSDNGSCYTASETTAFAKDMGFISCFTPVRSPESNGMAEAFVKTFKRDYVYVHDRPDAK
TVMAQLDQWFEDYNEFHPHKGLKMKSPRQFIRSQLLTASCPV
>Mature_282_residues
MKAIADTMGVARSRLVERMKPQEKSFRCRYNKADDAWLLPLIREIVDCRATYGYRRICALLNRKLERMGKPAVNHKRVYR
IMRQNGLLLARYTGKQRVLSHEGKVITLRSNLRWCSDGFEIPCWNGQVVRVAFALDCCDREVISHVATTGGITGEMVRDL
MTESVERRFGTVDLLPHRVEWLSDNGSCYTASETTAFAKDMGFISCFTPVRSPESNGMAEAFVKTFKRDYVYVHDRPDAK
TVMAQLDQWFEDYNEFHPHKGLKMKSPRQFIRSQLLTASCPV

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=247, Percent_Identity=52.2267206477733, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1789424, Length=247, Percent_Identity=52.2267206477733, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1789224, Length=247, Percent_Identity=52.2267206477733, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1788305, Length=247, Percent_Identity=52.2267206477733, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1787669, Length=247, Percent_Identity=52.2267206477733, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1786558, Length=247, Percent_Identity=52.2267206477733, Blast_Score=251, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 32487; Mature: 32487

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIADTMGVARSRLVERMKPQEKSFRCRYNKADDAWLLPLIREIVDCRATYGYRRICAL
CCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNRKLERMGKPAVNHKRVYRIMRQNGLLLARYTGKQRVLSHEGKVITLRSNLRWCSDGFE
HHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCEEEEECCCCCCCCCCC
IPCWNGQVVRVAFALDCCDREVISHVATTGGITGEMVRDLMTESVERRFGTVDLLPHRVE
CCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCHHHHH
WLSDNGSCYTASETTAFAKDMGFISCFTPVRSPESNGMAEAFVKTFKRDYVYVHDRPDAK
HCCCCCCEEECCCHHHHHHHCCHHEEECCCCCCCCCCHHHHHHHHHHHCEEEEECCCCHH
TVMAQLDQWFEDYNEFHPHKGLKMKSPRQFIRSQLLTASCPV
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKAIADTMGVARSRLVERMKPQEKSFRCRYNKADDAWLLPLIREIVDCRATYGYRRICAL
CCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNRKLERMGKPAVNHKRVYRIMRQNGLLLARYTGKQRVLSHEGKVITLRSNLRWCSDGFE
HHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCEEEEECCCCCCCCCCC
IPCWNGQVVRVAFALDCCDREVISHVATTGGITGEMVRDLMTESVERRFGTVDLLPHRVE
CCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCHHHHH
WLSDNGSCYTASETTAFAKDMGFISCFTPVRSPESNGMAEAFVKTFKRDYVYVHDRPDAK
HCCCCCCEEECCCHHHHHHHCCHHEEECCCCCCCCCCHHHHHHHHHHHCEEEEECCCCHH
TVMAQLDQWFEDYNEFHPHKGLKMKSPRQFIRSQLLTASCPV
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA