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The map label for this gene is dusB [H]

Identifier: 82701266

GI number: 82701266

Start: 136565

End: 137578

Strand: Direct

Name: dusB [H]

Synonym: Nmul_A0131

Alternate gene names: 82701266

Gene position: 136565-137578 (Clockwise)

Preceding gene: 82701265

Following gene: 82701267

Centisome position: 4.29

GC content: 58.09

Gene sequence:

>1014_bases
ATGAAAATCGGTTCTCATACTCTCAAAAACAACCTTATCGTGGCGCCTATGGCGGGAGTGACAGATCGCCCGTTCAGGCA
ATTGTGCAAAAGCATGGGCGCCGGAATGGCTGTGTCCGAAATGGTGTCGAGCAATTCGCTCCTCTGGGGTTCCGAGAAGA
CACGCCGCCGCGCCAATCATGAAGGCGAGGTGGATCCGATCTCGGTGCAGATCGCCGGCGCCGACCCGGCGATGATGGCG
GAAGCTGCGCGCTACAATGTCGCGCAAGGAGCCCAGATCATCGACATCAACATGGGTTGCCCTGCCAAGAAGATTTGTAA
TGTCATGGCAGGCTCTGCATTGCTGCAGGACCCGCCGCTGGTCGGGCGGATTCTGGATGCCGTCATAGGCGCGGTGAGGG
TGCCTGTCACCCTCAAGATTCGCACGGGGTGGGATACCCAGCACAAGAATGCGCTCTCCATTGCCCGCATTGCGGAGAAT
GCCGGCATCCAGGCGCTTGCTATCCATGGACGTACGCGCGCCTGTGCTTACACCGGCCATGCCGAATACGATACCATCGC
GGCAGTCAAGGCTGAGGTGCGGATTCCCGTTGTCGCCAATGGGGACATTACAACACCGGAAAAAGCAAAACACGTGCTTG
ACTACACGGGAGCGGATGCGGTCATGATCGGCCGCGCAGCACAAGGCCGCCCCTGGATTTTTCGCGAGATCGATCACTAT
CTGGCTACCGGCTCACACCTTCCGCTGCCTGAAGTAGCGGAGATTCACCGTGTACTCGTCGCACATTTGCACGATCTATA
TAGCTTCTATGGCGAGTATTCGGGGGTCCGCATCGCCCGCAAGCATATTTCCTGGTATACCAAAGGACTGGTCGGGTCAG
CGGGTTTCCGTCATGCCATGAACCAGCTGCAGTCTACGGACCAGCAGCTGTCTGCGGTTAACGACTTTTTCAGTGAGCTT
GCCGGCTACGGGCGGCGGTTGACCTACGTCGAGGCTGAGGAACTGGTGGCATGA

Upstream 100 bases:

>100_bases
AAAGCGGCAGGGCTGCTACCCTAATCTGCAATAGCTGTTAGAATTACGTCCATTCTCATCCCTTGCCCGTCGCCGCACTT
TGGCGGGCCAGTTCAGAATC

Downstream 100 bases:

>100_bases
GCATAATTAACGAAAACGATATCGCGCGGTGCGTACGCAAAACGGTCGAAGATTACTTTAACGATCTCGACGGGGAGAAG
CCGCATGCCATCCATGAAAT

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKIGSHTLKNNLIVAPMAGVTDRPFRQLCKSMGAGMAVSEMVSSNSLLWGSEKTRRRANHEGEVDPISVQIAGADPAMMA
EAARYNVAQGAQIIDINMGCPAKKICNVMAGSALLQDPPLVGRILDAVIGAVRVPVTLKIRTGWDTQHKNALSIARIAEN
AGIQALAIHGRTRACAYTGHAEYDTIAAVKAEVRIPVVANGDITTPEKAKHVLDYTGADAVMIGRAAQGRPWIFREIDHY
LATGSHLPLPEVAEIHRVLVAHLHDLYSFYGEYSGVRIARKHISWYTKGLVGSAGFRHAMNQLQSTDQQLSAVNDFFSEL
AGYGRRLTYVEAEELVA

Sequences:

>Translated_337_residues
MKIGSHTLKNNLIVAPMAGVTDRPFRQLCKSMGAGMAVSEMVSSNSLLWGSEKTRRRANHEGEVDPISVQIAGADPAMMA
EAARYNVAQGAQIIDINMGCPAKKICNVMAGSALLQDPPLVGRILDAVIGAVRVPVTLKIRTGWDTQHKNALSIARIAEN
AGIQALAIHGRTRACAYTGHAEYDTIAAVKAEVRIPVVANGDITTPEKAKHVLDYTGADAVMIGRAAQGRPWIFREIDHY
LATGSHLPLPEVAEIHRVLVAHLHDLYSFYGEYSGVRIARKHISWYTKGLVGSAGFRHAMNQLQSTDQQLSAVNDFFSEL
AGYGRRLTYVEAEELVA
>Mature_337_residues
MKIGSHTLKNNLIVAPMAGVTDRPFRQLCKSMGAGMAVSEMVSSNSLLWGSEKTRRRANHEGEVDPISVQIAGADPAMMA
EAARYNVAQGAQIIDINMGCPAKKICNVMAGSALLQDPPLVGRILDAVIGAVRVPVTLKIRTGWDTQHKNALSIARIAEN
AGIQALAIHGRTRACAYTGHAEYDTIAAVKAEVRIPVVANGDITTPEKAKHVLDYTGADAVMIGRAAQGRPWIFREIDHY
LATGSHLPLPEVAEIHRVLVAHLHDLYSFYGEYSGVRIARKHISWYTKGLVGSAGFRHAMNQLQSTDQQLSAVNDFFSEL
AGYGRRLTYVEAEELVA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=232, Percent_Identity=30.6034482758621, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI239788462, Length=234, Percent_Identity=30.3418803418803, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=32.0175438596491, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=30.6122448979592, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=31.304347826087, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=60.1246105919003, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1788462, Length=251, Percent_Identity=31.8725099601594, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI145693211, Length=302, Percent_Identity=26.8211920529801, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=244, Percent_Identity=30.327868852459, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=255, Percent_Identity=29.8039215686275, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17510279, Length=268, Percent_Identity=29.4776119402985, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=263, Percent_Identity=30.7984790874525, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=242, Percent_Identity=31.404958677686, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=26.6375545851528, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24585320, Length=245, Percent_Identity=33.469387755102, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24580595, Length=263, Percent_Identity=31.1787072243346, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920448, Length=263, Percent_Identity=31.1787072243346, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=28.695652173913, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45549423, Length=253, Percent_Identity=32.0158102766798, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36546; Mature: 36546

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGSHTLKNNLIVAPMAGVTDRPFRQLCKSMGAGMAVSEMVSSNSLLWGSEKTRRRANH
CCCCCCCHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCEEECCHHHHHHCCC
EGEVDPISVQIAGADPAMMAEAARYNVAQGAQIIDINMGCPAKKICNVMAGSALLQDPPL
CCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHCCCCH
VGRILDAVIGAVRVPVTLKIRTGWDTQHKNALSIARIAENAGIQALAIHGRTRACAYTGH
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEECCC
AEYDTIAAVKAEVRIPVVANGDITTPEKAKHVLDYTGADAVMIGRAAQGRPWIFREIDHY
CCHHHHHEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
LATGSHLPLPEVAEIHRVLVAHLHDLYSFYGEYSGVRIARKHISWYTKGLVGSAGFRHAM
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
NQLQSTDQQLSAVNDFFSELAGYGRRLTYVEAEELVA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHCC
>Mature Secondary Structure
MKIGSHTLKNNLIVAPMAGVTDRPFRQLCKSMGAGMAVSEMVSSNSLLWGSEKTRRRANH
CCCCCCCHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCEEECCHHHHHHCCC
EGEVDPISVQIAGADPAMMAEAARYNVAQGAQIIDINMGCPAKKICNVMAGSALLQDPPL
CCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHCCCCH
VGRILDAVIGAVRVPVTLKIRTGWDTQHKNALSIARIAENAGIQALAIHGRTRACAYTGH
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEECCC
AEYDTIAAVKAEVRIPVVANGDITTPEKAKHVLDYTGADAVMIGRAAQGRPWIFREIDHY
CCHHHHHEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
LATGSHLPLPEVAEIHRVLVAHLHDLYSFYGEYSGVRIARKHISWYTKGLVGSAGFRHAM
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
NQLQSTDQQLSAVNDFFSELAGYGRRLTYVEAEELVA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]