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The map label for this gene is intB [H]

Identifier: 82546482

GI number: 82546482

Start: 4227410

End: 4228675

Strand: Reverse

Name: intB [H]

Synonym: SBO_4174

Alternate gene names: 82546482

Gene position: 4228675-4227410 (Counterclockwise)

Preceding gene: 82546485

Following gene: 82546477

Centisome position: 93.56

GC content: 47.39

Gene sequence:

>1266_bases
ATGAAGCTCAACGCCAGACAGGTCGAGACCGCAAAGCCAAAAGACAAAACCTACAAAATGGCCGATGGTGGTGGTTTGTA
TCTTGAGGTTTCGGCCAAAGGTTCCAAATACTGGCGCATGAAATACAGACGTCCCTCTGACAAAAAAGAGGATCGTCTCG
CGTTTGGTGTTTGGCCTACTGTGACGCTTGCTCAGGCAAGAGCAAAGCGCGATCAAGCTAAAAAGCTGTTAGCGCAGGGC
ATTGATCCAAAAGCCGAACAGAAAGAAGCTCAGGCCGAGAATTCGGGGGCATATTCTTTCGAAACTATCGCTCGTGAATG
GCATGCTAGTAATAAGCGCTGGAGTGAAGATCACCGATCACGCGTTCTACGTTATCTTGAGCTTTATATCTTTCCTCATA
TTGGTTCATCTGACATCCGTCAGCTCAAAACTAGCCACCTATTAGCCCCGATTAAAAAAGTTGATGCCAGTGGTAAGCAT
GACGTTGCACAGCGCCTTCAGCAGCGTGTCACGGCTATTATGCGTTATGCAGTACAGAACGATTACATCGACTCAAATCC
AGCCAGTGATATGGCCGGTGCGCTATCGACAACCAAAGCGCGACATTACCCCGCTTTGCCCTCCAGCCGGTTCCCTGAGT
TTCTTGCTCGTCTTGCTGCATATCGTGGCCGTGTAATGACGCGGATCGCGGTTGAGCTTTCCTTACTAACTTTTGTGCGT
TCCAGTGAGTTACGTTTCGCGCGTTGGGATGAGTTCGACTTCGATAAATCTCTCTGGCGAGTACCTGCAAAACGAGAAGA
AATTAAAGGTGTGCGTTACTCGTACCGTGGCATGAAGATGAAAGAGGAGCATATCGTTCAGCTTAGTCGGCAGGCGATGA
TTTTGCTAAACCAGTTAAAGCAGATTAGTGGAGATAAAGAGCTGCTTTTTCCGGGTGATCATGACGCAACTAAGGTTATG
AGCGAAAACACGGTAAATAGCGCATTGCGTGCGATGGGCTATGACACGAAAACCGAAGTTTGCGGGCACGGGTTTAGAAC
GATGGCCCGTGGTGCGTTGGGCGAGTCCGGATTATGGAGTGAAGATGCTATAGAACGACAGTTGAGCCATTCAGAGCGTA
ATAATGTACGTGCTGCTTATATTCACACCTCTGAACATTTGGATGAACGTCGATTGATGGTTCAATGGTGGGCTGATTAT
CTCCAAGCAAATAAGGGCAAAACAATAACACCTTATGATTTTGCTAAAATAAACAAAAGTCGATAG

Upstream 100 bases:

>100_bases
TGCTTGCAGCGATTTTTTCGTCCTGCGTCTTCCGAGGTTGTCCGTTGAAATCCGGATATCATTGGGGGCATCTTAACTTC
GATTAGAAATGTGCCCCCAA

Downstream 100 bases:

>100_bases
TGAAAATAAACGCCTATAATATATAGGCGTTTATTTCTGTATTAAACACTTTGATCATAATCTTCCAGCAGCAACTTTTT
TTCGTACTTCATCTTTCCAA

Product: P4-type integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQG
IDPKAEQKEAQAENSGAYSFETIAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVM
SENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADY
LQANKGKTITPYDFAKINKSR

Sequences:

>Translated_421_residues
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQG
IDPKAEQKEAQAENSGAYSFETIAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVM
SENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADY
LQANKGKTITPYDFAKINKSR
>Mature_421_residues
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQG
IDPKAEQKEAQAENSGAYSFETIAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVM
SENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADY
LQANKGKTITPYDFAKINKSR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=419, Percent_Identity=35.0835322195704, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1788690, Length=401, Percent_Identity=33.9152119700748, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI145693166, Length=417, Percent_Identity=32.6139088729017, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 48395; Mature: 48395

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPT
CCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEECCCCH
VTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFETIAREWHASNKRWSEDHRS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHH
RVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKHDVAQRLQQRVTAIMRYAVQN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVQLSRQAMILLNQLK
CCCCCEEECCCCCCCHHHHHCCCHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QISGDKELLFPGDHDATKVMSENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWS
HHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCCCC
EDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADYLQANKGKTITPYDFAKINKS
HHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
R
C
>Mature Secondary Structure
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPT
CCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEECCCCH
VTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFETIAREWHASNKRWSEDHRS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHH
RVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKHDVAQRLQQRVTAIMRYAVQN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVQLSRQAMILLNQLK
CCCCCEEECCCCCCCHHHHHCCCHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QISGDKELLFPGDHDATKVMSENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWS
HHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCCCC
EDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADYLQANKGKTITPYDFAKINKS
HHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]