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The map label for this gene is intB [H]

Identifier: 82546472

GI number: 82546472

Start: 4215530

End: 4216723

Strand: Reverse

Name: intB [H]

Synonym: SBO_4164

Alternate gene names: 82546472

Gene position: 4216723-4215530 (Counterclockwise)

Preceding gene: 82546473

Following gene: 82546471

Centisome position: 93.29

GC content: 50.92

Gene sequence:

>1194_bases
ATGTCCTTACTTGTAAAACCTGGCGGTTCAAAGTATTGGCGATTTCGTTTCCGTTTTGGCGGTAAACAACATTTGATGGC
GTTCGGCGTTTACCCGGATGTTTCGCTGGCGGATGCGCGGAAGAAAAGGGAAGAGGCCAGAAAGCTGGTAGCTGCTGGTA
TCGATCCTCGTGAGCATAAGCGTGCTGTGAAAGAAGAGCAGGCGAAAGAGATTATTACTTTCGAGAAGGTTGCCAGAGAG
TGGCTCGTAACCAACCAAAAATGGTCGGAAGAGCATGCTAATCGTGTAAAAAAGAGCCTGGAGGACAATATCTTCCCGGC
AATTGGTTCCCACAATATTGCTGAACTGGGCACCCGCGATCTGTTAATTCCTATTAAAGCCGTGGAGAAATCCGGACGTC
TTGAAGTGGCTTCCCGCCTTCAACAGCGCACCACGGCCATCATGCGTTATGCAGTGCAAAGCGGGTTAATTGATTACAAT
CCGGCGCAAGAGATGGCGGGGGCGGTAGCTTCCAGTAATCGACAGCATCGTCCGGCGCTGGAGTTAAAGCGTATCCCGGA
ATTACTCCAGAAAATCGATGAATACACCGGCAGGCCACTAACCCGCTGGGCAACCGAACTTACCCTGCTTATTTTTATTC
GATCCAGTGAGCTGCGTTTTGCTCGCTGGTCAGAAATCGATTTTGAAACGTCAATGTGGACCATCCCGCCTGAGCGAGAA
CCTATTCCCGGTGTGAAACATTCTCAGCGAGGAGCAAAGATGCGTACACCTCATCTGGTGCCGCTTTCAAAGCAGGCGCT
AGCGATCCTGAAGCAGATAAAACAGTTCTGTGGCGAACATGAGCTGATTTTTATTGGCGATCACGATCCGAGTAAGCCCA
TGAGTGAAAACACGGTGAACAGTGCATTGCGGGTGATGGGCTATGACACCAAGGTTGAGGTTTGCGGCCACGGCTTCCGC
ACTATGGCCTGTAGCTCATTGATTGAGTCAGGGTTATGGTCGAAGGATGCGGTGGAAAGGCAGATGAGCCATATGGAACG
CAATTCGGTGAGGGCGGCGTATATCCATAAAGCGGAACATCTGGATGAGCGCAAGCTGATGTTGCAGTGGTGGGCTGATT
TTCTGGATGCGAACCGGGAGAAGGGGATTACGCCGTTTGACTATGCGAAGATCAATCGGGGTAATGGCGAGTGA

Upstream 100 bases:

>100_bases
AGATACCAATGGAAGGAAGACCATTATGGCCCTAACGGATACTAAAGTTCGCTCTGCGAAACCTGAGGAGAAAGAGTATT
CACTTGTTGACGGCGACGGC

Downstream 100 bases:

>100_bases
GTTATCTAAAAGCGCACATTCGCCTCTAAATTGAAAGCTGCTGACTTATTCAAACGAGGAATTGGTCAGCAGTGCCAAAT
AGCCGAGTTTTCACTAAGGT

Product: prophage P4 integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKVARE
WLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLEVASRLQQRTTAIMRYAVQSGLIDYN
PAQEMAGAVASSNRQHRPALELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPERE
PIPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYDTKVEVCGHGFR
TMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLMLQWWADFLDANREKGITPFDYAKINRGNGE

Sequences:

>Translated_397_residues
MSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKVARE
WLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLEVASRLQQRTTAIMRYAVQSGLIDYN
PAQEMAGAVASSNRQHRPALELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPERE
PIPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYDTKVEVCGHGFR
TMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLMLQWWADFLDANREKGITPFDYAKINRGNGE
>Mature_396_residues
SLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKVAREW
LVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLEVASRLQQRTTAIMRYAVQSGLIDYNP
AQEMAGAVASSNRQHRPALELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREP
IPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYDTKVEVCGHGFRT
MACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLMLQWWADFLDANREKGITPFDYAKINRGNGE

Specific function: Unknown

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=395, Percent_Identity=37.2151898734177, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI145693166, Length=379, Percent_Identity=33.7730870712401, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788690, Length=382, Percent_Identity=32.1989528795811, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45495; Mature: 45364

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHK
CCEEECCCCCCEEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
RAVKEEQAKEIITFEKVAREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
LLIPIKAVEKSGRLEVASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPAL
EEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHH
ELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPERE
HHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCCCCEEECCCCCCCCCEEECCCCCC
PIPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
SALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEH
HHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LDERKLMLQWWADFLDANREKGITPFDYAKINRGNGE
HHHHHHHHHHHHHHHHCCHHCCCCCCCHHCCCCCCCC
>Mature Secondary Structure 
SLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHK
CEEECCCCCCEEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
RAVKEEQAKEIITFEKVAREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
LLIPIKAVEKSGRLEVASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPAL
EEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHH
ELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPERE
HHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCCCCEEECCCCCCCCCEEECCCCCC
PIPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
SALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEH
HHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LDERKLMLQWWADFLDANREKGITPFDYAKINRGNGE
HHHHHHHHHHHHHHHHCCHHCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]