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The map label for this gene is hsdM [C]

Identifier: 82546467

GI number: 82546467

Start: 4210455

End: 4212089

Strand: Direct

Name: hsdM [C]

Synonym: SBO_4159

Alternate gene names: 82546467

Gene position: 4210455-4212089 (Clockwise)

Preceding gene: 82546466

Following gene: 82546468

Centisome position: 93.16

GC content: 50.89

Gene sequence:

>1635_bases
ATGATTACTGGCGACTTAAAAAGTAAAATCGACGGACTATGGGAAGATTTCTGGGTGGGTGGTATCACCAACCCGCTGAC
CGTAATCGAACAGATCACCTATCTGATGTATTCCCGGATGCTGGATACCCAGGAACAACGCGACGAAAAGCGTAAACAAA
TCGCGGGTATTGATTTTAAACCGCGTTTTGCGCCAGAACAGCAGGAGTTCCGTTTCAGTCACTATAGCAACCTTGGCTCG
GATGAGATGATGGAAGTGGTGCGCGATGGCGTATTCCAGCATTTCCGTCAGCTCGGCCAGGCCGATGCTTCGAAGGTGAC
GCTGCTGGGCAACTTTATGAAAGATGCTCGTCTGGAGATTGTTAAGCCGTCATTGCTGACCAAAGCGGTTGAGGTAATCA
AAAACCTGCCATTGGATCGCGGCGACACCAAAGGCGACCTTTACGAATACCTGTTAAGCAAGCTGACAACTGCCGGAATC
AACGGACAGTTCCGCACACCGCGCCACATTATCCGCACGATGGTTGAAATGATGGAGCCGAACCCGGCCCGCGGCGAGAC
GATTTGCGATCCCGCCTGTGGCACCGGTGGTTTTCTGGCAACCAGCTATGAATATCTGCTGGAGAAGTACAGCTCGCTGG
AATCCATTCATACTGAGATTGGCACCAACGAACGTGGCGAGCTGGAAGAGCAAAAAATCTTTACCGGCGATCTGCTGACA
CCGTGGCGTAACCATGTGGATAACAACATGTTCCACGGTTACGACTTTGACACCACAATGCTACGTATCGCCGCCATGAA
CCTGATTATGCACGGCGTGGATGCGCCTGATATCCACTATCAGGACACAATGAGCCAGAGTTTCAGCACAAACTTCCCGC
AGGCCAGTAAAAACGCCTTCAACCTGATTCTGGCGAACCCGCCGTTTACCGGTTCACTGGACGAGGAAGATATCGACTCC
ACGCTGTCGGCAATGGTGAAAACCAAAAAAACCGAACTACTGTTCCTGGCGCGTATTCTGCAAATGCTGAAAGTGGGCGG
GCGCAGTGCCACTATCGTGCCGCAGGGCGTGCTGTTTGGCTCTAGCAAGGCGCACCAGTCACTTCGCAAAACGCTGGTGG
AAGATAACCAACTGGAAACGGTGATCAATCTGCCTTCTGGTGTATTTAAACCTTACGCTGGCGTGGCGACGGCGATCCTG
ATCTTTACCAAAGGCGGTCAAACGGATGAGGTCTGGTTCTACGATCTACAAAATGACGGCTACAGCCTGGATGATAAGCG
CAACCCGATAAAAGACAACGATCTGCCGCATCTGCTGGCAAGCTGGAAGCATTACCGTACTTTACGCGGGCTACCGGTTG
ATAACTTTATGGGTAAGAAGTTAGCCTCGTTGCTTAAACAGCAGTACCCGGAAGGGATTAATGCTGGCGTTGATTTTAAA
GATCGCACGCAGGCGGCGTTTGTTGTACCGAAAGCGGATATTGCTGCGCAGAAATACGATCTATCCATCAACCGTTATAA
AGAAGTCGTGTATCAGGCGGAGGAATATGAAGATCCGAAGGTGATATTGAAGCGGTTAAAGGATCTGGAAAAAGAGATTC
TGGCGGATTTGGATGAGCTGGAGGGGATGCTGTGA

Upstream 100 bases:

>100_bases
AGAGCTGGGTGTTGTGACCGACGCAGCGACGGTATAATACACGCATGACAAGCCCCGGCAGCGGGGCTTTTGTATTTAAA
ACAGGATATAAACGCTAACA

Downstream 100 bases:

>100_bases
GTTCGGCGATGGTGAAGTTAGGGGATGTTATTAATGTTCACTACGGAAAAGCATTAAAAGCTGATCAGCGTGTATCGAAT
GGAAGTGTCCATGTGTTTGG

Product: type I restriction enzyme M protein

Products: NA

Alternate protein names: M.BthVORF4518P [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQEQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGS
DEMMEVVRDGVFQHFRQLGQADASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHTEIGTNERGELEEQKIFTGDLLT
PWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAPDIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDS
TLSAMVKTKKTELLFLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPYAGVATAIL
IFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRGLPVDNFMGKKLASLLKQQYPEGINAGVDFK
DRTQAAFVVPKADIAAQKYDLSINRYKEVVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML

Sequences:

>Translated_544_residues
MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQEQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGS
DEMMEVVRDGVFQHFRQLGQADASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHTEIGTNERGELEEQKIFTGDLLT
PWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAPDIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDS
TLSAMVKTKKTELLFLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPYAGVATAIL
IFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRGLPVDNFMGKKLASLLKQQYPEGINAGVDFK
DRTQAAFVVPKADIAAQKYDLSINRYKEVVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML
>Mature_544_residues
MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQEQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGS
DEMMEVVRDGVFQHFRQLGQADASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHTEIGTNERGELEEQKIFTGDLLT
PWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAPDIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDS
TLSAMVKTKKTELLFLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPYAGVATAIL
IFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRGLPVDNFMGKKLASLLKQQYPEGINAGVDFK
DRTQAAFVVPKADIAAQKYDLSINRYKEVVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=458, Percent_Identity=27.7292576419214, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 61630; Mature: 61630

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCC
>Mature Secondary Structure
MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQEQRDEKRKQIAGIDFK
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQADASKVTLLGNFMKDARLEI
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGINGQFRTPRHIIRTMVEMMEP
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
NPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHTEIGTNERGELEEQKIFTGDLLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
PWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAPDIHYQDTMSQSFSTNFPQASKNAF
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCE
NLILANPPFTGSLDEEDIDSTLSAMVKTKKTELLFLARILQMLKVGGRSATIVPQGVLFG
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEC
SSKAHQSLRKTLVEDNQLETVINLPSGVFKPYAGVATAILIFTKGGQTDEVWFYDLQNDG
CHHHHHHHHHHHHCCCHHHHHHCCCCHHHCHHHHHHHEEHEEECCCCCCCEEEEEECCCC
YSLDDKRNPIKDNDLPHLLASWKHYRTLRGLPVDNFMGKKLASLLKQQYPEGINAGVDFK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DRTQAAFVVPKADIAAQKYDLSINRYKEVVYQAEEYEDPKVILKRLKDLEKEILADLDEL
CCCCEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
EGML
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]