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The map label for this gene is yjbN [H]

Identifier: 82546382

GI number: 82546382

Start: 4110953

End: 4111990

Strand: Reverse

Name: yjbN [H]

Synonym: SBO_4068

Alternate gene names: 82546382

Gene position: 4111990-4110953 (Counterclockwise)

Preceding gene: 82546383

Following gene: 161984802

Centisome position: 90.98

GC content: 53.47

Gene sequence:

>1038_bases
ATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCG
GTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGC
TGTATACCGAAATGGTGACCACAGGGGCGATTATTCACGGTAAAGGTGATTACCTGGCGTACAGTGAAGAAGAACATCCG
GTAGCGTTGCAACTCGGCGGTAGCGATCCGGCGGCGCTGGCACAGTGTGCGAAGCTGGCAGAAGCGCGTGGATATGATGA
GATCAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGC
TGGTTGCCGATTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGATGAC
CAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGCGAGTGTGGGATGTTCATCATCCATGC
ACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAAC
TGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAA
CATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGG
TTCCTCGGATACCGATGCCTATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGA
CGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTGTTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTA
AGTGAAAATGCCCATAAAGCGGGTGCAGACATTAATGTGCTGGAACACGCGCTCAAACTGGTGGCGGATAAGCGTTAA

Upstream 100 bases:

>100_bases
TGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAA
AATAATCTCTACATTTGAAA

Downstream 100 bases:

>100_bases
CTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATT
TTTGGCATGATTCTTGTAAT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHP
VALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ
HMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYL
SENAHKAGADINVLEHALKLVADKR

Sequences:

>Translated_345_residues
MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHP
VALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ
HMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYL
SENAHKAGADINVLEHALKLVADKR
>Mature_345_residues
MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHP
VALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ
HMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYL
SENAHKAGADINVLEHALKLVADKR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=308, Percent_Identity=23.7012987012987, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI145693211, Length=330, Percent_Identity=99.3939393939394, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=238, Percent_Identity=25.2100840336134, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38444; Mature: 38444

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVT
CCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEHHHH
TGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQ
HCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHC
NGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGEC
CCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCC
GMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ
CEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
HMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGHIT
HHCCHHCCHHHHCCCCEEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
RHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR
HHHHHHHHCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVT
CCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEHHHH
TGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQ
HCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHC
NGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGEC
CCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCC
GMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ
CEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
HMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGHIT
HHCCHHCCHHHHCCCCEEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
RHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR
HHHHHHHHCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]