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The map label for this gene is metE [H]

Identifier: 82546180

GI number: 82546180

Start: 3863180

End: 3865441

Strand: Direct

Name: metE [H]

Synonym: SBO_3841

Alternate gene names: 82546180

Gene position: 3863180-3865441 (Clockwise)

Preceding gene: 82546178

Following gene: 82546181

Centisome position: 85.47

GC content: 55.61

Gene sequence:

>2262_bases
ATGACAATATTGAATCACACCCTCGGTTTCCCTCGCGTTGGCCTGCGTCGCGAGCTGAAAAAAGCGCAAGAGAGTTATTG
GGCAGGGAACTCCACGCGTGAAGAACTGCTGGCGGTAGGGCGTGAATTGCGTGCTCGTCACTGGGATCAACAAAAGCAAG
CGGGTATCGACCTGCTGCCGGTGGGCGATTTTGCCTGGTACGATCATGTACTGACCACCAGTCTGCTGCTGGGTAACGTT
CCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATTGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGG
CGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAATTCGTTAAAGGCC
AACAGTTCAAACTGACCTGGACGCAGCTGCTGGAGGAAGTGGACGAGGCGCTGGCGCTGGGCCACAACGTTAAGCCTGTG
CTGCTGGGGCCGGTTACCTGGCTGTGGCTGGGGAAAGTGAAAGGTGAACAGTTTGACCGCCTGAGCCTGCTGAACGACAT
TCTGCCGGTTTATCAGCAAGTGCTGGCAGAACTGGAGAAACGCGGCATCGAGTGGGTACAGATTGATGAACCCGCGCTGG
TACTGGAACTACCACAGGCGTGGCTGGACGCATACAAACCCGCTTACGACGCGCTCCAGGGACAGGTGAAACTGCTGCTG
ACTACCTATTTTGAAGGCGTTACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGACTGCATGTGGACCTTGT
ACATGGTAAAGATGGCGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGCTGCTGTCTGCGGGACTGATCAATGGTC
GTAACGTCTGGCGCGCCGATCTTACTGAGAAATATGCGCAAATTAAGGACATTGTCGGCAAACGTGATTTGTGGGTGGCA
TCTTCCTGCTCGTTGCTGCACAGCCCCATCGACCTGAGCGTGGAAACGCGTCTTGATGCAGAAGTGAAAAGCTGGTTTGC
CTTCGCCTTACAAAAATGCCATGAACTGGCACTGCTGCGCGATGCGCTGAACAGTGGTGACACGGCAGCTCTGGCAGAGT
GGAGCGCCCCAATTCAGGCGCGTCGTCACTCTACTCGTGTACATAATCCGGCGGTAGAAAAGCGTCTGGCGGCGATCACC
GCCCAGGACAGCCAGCGTGCGAATGTCTATGAAGTGCGTGCTGAAGCCCAGCGTGCGCGTTTTAAACTGCCAGCGTGGCC
GACCACCACGATTGGTTCCTTCCCGCAAACCACGGAAATTCGTACCCTGCGTCTGGATTTCAAAAAGGGCAATCTCGACG
CCAACAACTACCGCACGGGCATTGCGGAACATATCAAGCAGGCCATTGTTGAGCAGGAACGTTTGGGACTGGATGTGCTG
GTACATGGCGAGGCCGAGCGTAATGACATGGTGGAATACTTTGGCGAGCACCTCGACGGATTTGTCTTTACGCAAAACGG
TTGGGTACAGAGCTACGGTTCCCGCTGCGTGAAGCCACCGATTGTTATTGGTGACGTTAGTCGCCCGGCACCGATTACCG
TGGAGTGGGCGAAGTATGCGCAATCTCTGACTGATAAACCGGTGAAAGGGATGCTGACTGGCCCGGTGACCATTCTCTGC
TGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAAATTGCGCTGGCGCTGCGTGATGAAGTGGCGGA
CCTGGAAGCCGCAGGAATTGGCATCATCCAGATTGATGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACT
GGGATGCATATCTCCAGTGGGGCGTGGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACT
CACATGTGTTATTGCGAGTTCAACGACATCATGGATTCCATTGCGGCACTGGATGCAGACGTCATCACCATCGAAACCTC
GCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCGAATGAAATCGGTCCAGGCGTCTATGACATTC
ACTCGCCAAACGTACCGAGCGTAGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTG
TGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGC
GCAGAATTTGCGTCGGGGATGA

Upstream 100 bases:

>100_bases
CCCCGGTCACTTACTTCAGTAAGCTCCCGGGGATGAATAAACTTGCCGCCTTCCCTAAATTCAAAATCCATAGGATTTAC
ATATAATTAGAGGAAGAAAA

Downstream 100 bases:

>100_bases
TGAATATTAACGAGTCACTGTAAATTGCAGTACGATGAAAGAGGCAAAACTGCCTGATACCAGGCCTACATAATTTATGC
AATTTATTGAATTTTTGAGT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNV
PARHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVA
SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAIT
AQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILC
WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHT
HMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG

Sequences:

>Translated_753_residues
MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNV
PARHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVA
SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAIT
AQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILC
WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHT
HMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG
>Mature_752_residues
TILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVP
ARHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPVL
LGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLLT
TYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVAS
SCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITA
QDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVLV
HGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCW
SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTH
MCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLW
VNPDCGLKTRGWPETRAALANMVQAAQNLRRG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=753, Percent_Identity=99.33598937583, Blast_Score=1537, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=761, Percent_Identity=48.8830486202365, Blast_Score=711, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 84660; Mature: 84529

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPVLLGPVTWLWLGKVKGEQFDR
CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCHHHH
LSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHCHHHHHHHHHHHHHH
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAELHKRLPSDWLLSAGLINGRNVWRAD
HHHHCCCCCCCCCEEECCCCCCEEEEEECCCCHHHHHHHCCHHHHHHCCCCCCCCCCHHH
LTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR
HHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHH
DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR
HHHCCCCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
EECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYA
EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCCEEEHHHHHH
QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE
HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD
HHHHCCCCCCCCCHHHHHHHCCHHEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCC
VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPVLLGPVTWLWLGKVKGEQFDR
CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCHHHH
LSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHCHHHHHHHHHHHHHH
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAELHKRLPSDWLLSAGLINGRNVWRAD
HHHHCCCCCCCCCEEECCCCCCEEEEEECCCCHHHHHHHCCHHHHHHCCCCCCCCCCHHH
LTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR
HHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHH
DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR
HHHCCCCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
EECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYA
EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCCEEEHHHHHH
QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE
HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD
HHHHCCCCCCCCCHHHHHHHCCHHEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCC
VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA