Click here to switch to the map view.

The map label for this gene is insF1 [C]

Identifier: 82546071

GI number: 82546071

Start: 3729756

End: 3730130

Strand: Direct

Name: insF1 [C]

Synonym: SBO_3715

Alternate gene names: 82546071

Gene position: 3729756-3730130 (Clockwise)

Preceding gene: 82546070

Following gene: 82546073

Centisome position: 82.52

GC content: 50.13

Gene sequence:

>375_bases
ATGACGAAAGAGTTGACGGGTAAAGCCCTGTTTATGGCGCTCAGGAGCCAGCGCCCACCTGCCGGGCTAATCCACCACAC
CGATCGAGGTTCACAGTACTGCGCATACGATTACCGGGTCATACAGGAGCAGTCTGGTCTGAAAACATCAATGTCGCGTA
AAGGTAACTGTTACGACAACGCTCCGATGGAAAGCTTCTGGGGAACGCTGAAAAATGAAAGCCTGAGCCACTATCGTTTT
AAGAGCCGGGATGAAGCCATCTCAGTAATACGGGAATACATTGAGATTTTCTACAATCGTCAGCGTCGTCACTCTCGTCT
GGGGAATATCTCCCCGGCAGCCTTCAGGGAAAAATATCATCAGATGGCTGCTTAA

Upstream 100 bases:

>100_bases
AGATATAACGTATGTTGCCACCCGGGAAGGATGGCTGTATCTCGCCGGCGTCAAAGATGTTTATACGTGCGAAATTGTCG
GTTACGCCATGGGAGAGTGC

Downstream 100 bases:

>100_bases
AAAAAGAACAAATGGTAGTGTCCGCTATTGCCAGTACACCTCATACCACAGAGAATACCAAGGAAGATGGCATTTAGAAT
TTGCAATTCCAGCTCAACCG

Product: IS600 ORF2

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 124; Mature: 123

Protein sequence:

>124_residues
MTKELTGKALFMALRSQRPPAGLIHHTDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRF
KSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Sequences:

>Translated_124_residues
MTKELTGKALFMALRSQRPPAGLIHHTDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRF
KSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
>Mature_123_residues
TKELTGKALFMALRSQRPPAGLIHHTDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFK
SRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=118, Percent_Identity=46.6101694915254, Blast_Score=103, Evalue=3e-24,
Organism=Escherichia coli, GI1787263, Length=118, Percent_Identity=46.6101694915254, Blast_Score=103, Evalue=3e-24,
Organism=Escherichia coli, GI1786753, Length=118, Percent_Identity=46.6101694915254, Blast_Score=103, Evalue=3e-24,
Organism=Escherichia coli, GI1786570, Length=118, Percent_Identity=46.6101694915254, Blast_Score=103, Evalue=3e-24,
Organism=Escherichia coli, GI1786490, Length=118, Percent_Identity=46.6101694915254, Blast_Score=103, Evalue=3e-24,
Organism=Escherichia coli, GI1789981, Length=109, Percent_Identity=38.5321100917431, Blast_Score=75, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 14499; Mature: 14367

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKELTGKALFMALRSQRPPAGLIHHTDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDN
CCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCC
APMESFWGTLKNESLSHYRFKSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYH
CCHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QMAA
HHCC
>Mature Secondary Structure 
TKELTGKALFMALRSQRPPAGLIHHTDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDN
CCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCC
APMESFWGTLKNESLSHYRFKSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYH
CCHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QMAA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]