Click here to switch to the map view.

The map label for this gene is yhiE [H]

Identifier: 82545876

GI number: 82545876

Start: 3503932

End: 3504459

Strand: Direct

Name: yhiE [H]

Synonym: SBO_3511

Alternate gene names: 82545876

Gene position: 3503932-3504459 (Clockwise)

Preceding gene: 82545875

Following gene: 82545877

Centisome position: 77.52

GC content: 32.58

Gene sequence:

>528_bases
ATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAAT
CAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTG
ATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAACTA
AAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTT
GCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAA
GCAATATCACGTTGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGTTGAAACTA
AGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAG

Upstream 100 bases:

>100_bases
GTTCTTATAGGCGTTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATGTTCGAAACGA
TAACGGCTAAGGAGCAAGTT

Downstream 100 bases:

>100_bases
TCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTA
AAGGAGAGGCTAAAACGACT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKL
KAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
RRMSDIVTLGITSYF

Sequences:

>Translated_175_residues
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKL
KAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
RRMSDIVTLGITSYF
>Mature_175_residues
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKL
KAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
RRMSDIVTLGITSYF

Specific function: Regulates the expression of several genes involved in acid resistance. Required for the expression of gadA and gadBC, among others, regardless of media or growth conditions. Binds directly to the 20 bp GAD box found in the control regions of both loci. Co

COG id: COG2771

COG function: function code K; DNA-binding HTH domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789928, Length=175, Percent_Identity=99.4285714285714, Blast_Score=350, Evalue=2e-98,
Organism=Escherichia coli, GI1789923, Length=167, Percent_Identity=23.9520958083832, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991
- InterPro:   IPR013022 [H]

Pfam domain/function: PF00196 GerE [H]

EC number: NA

Molecular weight: Translated: 20626; Mature: 20626

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKF
CEEEEECCCHHHHHHEEECCCCCEEEECCHHHHHHHCCCCEEEEEEHHHHHCCCHHHHHH
LEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQ
HHHCCCCEEEEECCCHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCEEEECCH
FKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF
HHHHHHHHHCCCCCEEEEEEEECHHEEEEEEEEEEEEEEHHHHHHEEEEEEECCC
>Mature Secondary Structure
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKF
CEEEEECCCHHHHHHEEECCCCCEEEECCHHHHHHHCCCCEEEEEEHHHHHCCCHHHHHH
LEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQ
HHHCCCCEEEEECCCHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCEEEECCH
FKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF
HHHHHHHHHCCCCCEEEEEEEECHHEEEEEEEEEEEEEEHHHHHHEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]