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The map label for this gene is arsB [H]

Identifier: 82545866

GI number: 82545866

Start: 3493874

End: 3495184

Strand: Direct

Name: arsB [H]

Synonym: SBO_3500

Alternate gene names: 82545866

Gene position: 3493874-3495184 (Clockwise)

Preceding gene: 82545865

Following gene: 82545867

Centisome position: 77.3

GC content: 53.62

Gene sequence:

>1311_bases
GTGTTTTATCCAGGAGGCATTATGTTACTGGCAGGCGCTATCTTTGTCCTGACCATTGTATTGGTTATCTGGCAGCCGAA
AGGTTTAGGCATCGGCTGGAGTGCAACGCTCGGCGCAGTACTGGCGTTAGTTACGGGCGTGGTCCATCCGGGTGATATTC
CGGTGGTGTGGAATATCGTCTGGAACGCGACGGCTGCGTTTATCGCCGTCATTATCATCAGCCTGCTGCTGGATGAGTCC
GGCTTTTTTGAATGGGCGGCGCTGCACGTCTCACGCTGGGGTAATGGTCGTGGTCGCTTGCTGTTTACCTGGATTGTCCT
GCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCAGCGCTTATTTTGACGCCGATTGTCATCGCCATGCTGC
TGGCTTTAGGATTCAGTAAAGGCACGACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCG
CTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATACGCCTCGGTGATGGT
GCCGGTGGATATCGCCGCGATTGTTGCCACGCTGGTGATGCTACATCTCTATTTTCGCAAAGATATTCCGCAGAACTACG
ATATGGCGCTGCTGAAATCTCCCGCAGAAGCGATCAAAGATCCTGCTACGTTCAAAACTGGCTGGGTTGTTTTACTGCTT
CTGCTGGTGGGATTTTTCGTCCTGGAACCGCTCGGCATTCCGGTGAGCGCCATTGCAGCTGTGGGCGCGCTGATATTATT
TGTCGTCGCTAAACGCGGTCATGCGATTAATACGGGTAAAGTCCTGCGCGGTGCCCCCTGGCAAATTGTCATCTTCTCGC
TCGGCATGTATCTGGTGGTTTATGGCCTGCGCAATGCCGGATTAACGGAATATCTTTCTGGCGTACTCAACGTGCTGGCG
GATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGAC
GGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACGGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGA
TTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACACTACTCTGGCTGCACGTACTTTCGCAGAAG
AATATGACTATCAGCTGGGGATATTACTTCCGTACAGGGATTATCATGACCCTGCCTGTGCTGTTTGTGACGCTGGCTGC
GCTGGCGCTACGTCTCTCTTTCACTTTGTAA

Upstream 100 bases:

>100_bases
TCAGGCAATTGTCCGCAACCTGGCTCGACAAAACTGTTCCGCAGACAGTAAGAACATTTGCAGTTAAAAAATTTAGCTAA
ACACATATGTATTTTCGCAT

Downstream 100 bases:

>100_bases
TGAGATACTGATATGAGCAACATTACCATTTATCACAACCCGGCCTGCGGCACGTCGCGTAATACGCTGGAGATGATCCG
CAACAGCGGCACAGAACCGA

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MFYPGGIMLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDES
GFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLP
LIVSNLVNIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLL
LLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLA
DNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQK
NMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL

Sequences:

>Translated_436_residues
MFYPGGIMLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDES
GFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLP
LIVSNLVNIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLL
LLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLA
DNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQK
NMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL
>Mature_436_residues
MFYPGGIMLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDES
GFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLP
LIVSNLVNIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLL
LLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLA
DNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQK
NMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=429, Percent_Identity=100, Blast_Score=829, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 46264; Mature: 46264

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYPGGIMLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIV
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHH
WNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
AALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHH
EYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHH
LLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTA
HHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCH
SGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPV
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
LFVTLAALALRLSFTL
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFYPGGIMLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIV
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHH
WNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
AALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHH
EYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHH
LLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTA
HHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCH
SGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPV
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
LFVTLAALALRLSFTL
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]