Click here to switch to the map view.

The map label for this gene is insB [H]

Identifier: 82545721

GI number: 82545721

Start: 3338441

End: 3338881

Strand: Direct

Name: insB [H]

Synonym: SBO_3352

Alternate gene names: 82545721

Gene position: 3338441-3338881 (Clockwise)

Preceding gene: 82545719

Following gene: 82545722

Centisome position: 73.86

GC content: 54.42

Gene sequence:

>441_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAAT
CACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACATCTTCGGTGAACGCACTCTG
GCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCGCTGTATGA
ATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGAC
AACATCTGGCAAGGCTGGGACGGAAGGTAAGCGTAAACTGA

Upstream 100 bases:

>100_bases
GCTCTCATTGCCGTAAAACATGGAAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
CCGCCGTATGTAGCCATTAAGCCTGTATTGGTAACGTAGGTGCCCACCTTTTCAACTAGTGGACACCTGTTATGGAACAG
AAAGCATTATCTGCAGAACC

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHIFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKVSVN

Sequences:

>Translated_146_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHIFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKVSVN
>Mature_145_residues
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHIFGERTLA
TLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKVSVN

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=142, Percent_Identity=88.7323943661972, Blast_Score=269, Evalue=6e-74,
Organism=Escherichia coli, GI1788203, Length=142, Percent_Identity=88.7323943661972, Blast_Score=269, Evalue=6e-74,
Organism=Escherichia coli, GI1786203, Length=142, Percent_Identity=88.7323943661972, Blast_Score=269, Evalue=6e-74,
Organism=Escherichia coli, GI1786470, Length=142, Percent_Identity=87.3239436619718, Blast_Score=265, Evalue=1e-72,
Organism=Escherichia coli, GI1786459, Length=142, Percent_Identity=87.3239436619718, Blast_Score=265, Evalue=1e-72,
Organism=Escherichia coli, GI1787223, Length=142, Percent_Identity=86.6197183098592, Blast_Score=264, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 17225; Mature: 17094

Theoretical pI: Translated: 11.13; Mature: 11.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVVAHIFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKVSVN
HHHHHHHCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVVAHIFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKVSVN
HHHHHHHCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]