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The map label for this gene is ygaF [H]

Identifier: 82545259

GI number: 82545259

Start: 2863028

End: 2864362

Strand: Reverse

Name: ygaF [H]

Synonym: SBO_2859

Alternate gene names: 82545259

Gene position: 2864362-2863028 (Counterclockwise)

Preceding gene: 161984872

Following gene: 82545258

Centisome position: 63.37

GC content: 57.0

Gene sequence:

>1335_bases
GTGGCTATTTCGCTTACGCCACTCACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGT
GATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTTTATCCGGATGCCCGCATTGCGTTGC
TGGAAAAAGAGTCCGGCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCC
GGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGACATTCGCTATGA
CAACTGCGGCAAGATGCTGATCGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCGTTGTGGGAACGCACGGCGGCGA
ACGGTATCGAGCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTT
GTGCCGTCCAGCGGCATTGTCAGCTATCGCGAAGTCACAGCGGCAATGGCAAAAATCTTCCAGGCCAGAGGCGGTGAGAT
TATTTATAACGCCGAAGTCAGCGCCCTCAGTGAGCATAAAAACGGCGTGGTCGTCCGAACCCGTCAGGGCGGCGAATATG
AAGCATCGACGCTGATTAGCTGTTCCGGGCTGATGGCTAACCGGCTGGTGAAAATGCTCGGCCTCGAACCGGGTTTTATT
ATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATAACCAGATTGTTAACCATCTGATTTACCCCATCCC
TGACCCGGCAATGCCGTTTTTGGGCGTGCATCTCACCCGCATGATCGACGGCAGCGTGACCGTCGGGCCAAACGCGGTGC
TGGCTTTTAAACGCGAAGGCTATCGCAAGCGCGACTTCTCATTTAGCGACACGCTGGAAATTTTAGGCTCGTCGGGGATT
CGCCGGGTGCTGCAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCT
GGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGC
CGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCA
GCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACG
CACGCTGCGAGCGACACGTAGTGTGGATGCCTTACACGCCGCATTTAATCAATAA

Upstream 100 bases:

>100_bases
CCCGTTGGCAAATTCCTGTTGATAAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCG
CCGTGAACTGATGCGTCAGC

Downstream 100 bases:

>100_bases
CCTTTGAAAACAGGACGTAACAATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTTGATTAACGGGGAATGGC
TGGACGCCAACAATGGCGGG

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTP
GSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERMRALWERTAANGIEREWLNAEELREREPNITGLGGIF
VPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGI
RRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPA
ATSAIPIGAHIVSKVQTLLASQSNPGRTLRATRSVDALHAAFNQ

Sequences:

>Translated_444_residues
MAISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTP
GSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERMRALWERTAANGIEREWLNAEELREREPNITGLGGIF
VPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGI
RRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPA
ATSAIPIGAHIVSKVQTLLASQSNPGRTLRATRSVDALHAAFNQ
>Mature_443_residues
AISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPG
SLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERMRALWERTAANGIEREWLNAEELREREPNITGLGGIFV
PSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFII
CPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIR
RVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAA
TSAIPIGAHIVSKVQTLLASQSNPGRTLRATRSVDALHAAFNQ

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=398, Percent_Identity=40.4522613065327, Blast_Score=315, Evalue=5e-86,
Organism=Escherichia coli, GI87082148, Length=422, Percent_Identity=97.39336492891, Blast_Score=842, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=42.14463840399, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24585081, Length=407, Percent_Identity=38.3292383292383, Blast_Score=278, Evalue=7e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 48666; Mature: 48535

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGP
CEEEECCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
ACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLE
CHHHCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHCCCCCCCCCCCCCEEEEECCCC
MERMRALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIF
HHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHH
QARGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
HHCCCCEEEECCHHHHHHCCCCEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEE
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREG
EECCCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCC
YRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDL
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLA
CCCCCCCEEEEECCCCCEEHHEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
SQSNPGRTLRATRSVDALHAAFNQ
CCCCCCCEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
AISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGP
EEEECCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
ACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLE
CHHHCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHCCCCCCCCCCCCCEEEEECCCC
MERMRALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIF
HHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHH
QARGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
HHCCCCEEEECCHHHHHHCCCCEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEE
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREG
EECCCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCC
YRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDL
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLA
CCCCCCCEEEEECCCCCEEHHEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
SQSNPGRTLRATRSVDALHAAFNQ
CCCCCCCEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]