Click here to switch to the map view.

The map label for this gene is hycH

Identifier: 82545207

GI number: 82545207

Start: 2808400

End: 2808810

Strand: Direct

Name: hycH

Synonym: SBO_2800

Alternate gene names: 82545207

Gene position: 2808400-2808810 (Clockwise)

Preceding gene: 82545206

Following gene: 82545208

Centisome position: 62.14

GC content: 53.04

Gene sequence:

>411_bases
ATGAGTGAAAAGGTGGTGTTCAGTCAACTGAGCCGTAAATTTATTGATGAGAACGATGCCACGCCCGCCGAGGCGCAGCA
GGTGGTCTATTACAGCCTGGCGATTGGTCACCACCTTGGGGTTATCGATTGCCTGGAAGCGGCGCTCACCTGCCCGTGGG
ATGAATATCTGGCATGGATTGCCACTCTGGAGGCGGGCAGTGAAGCCCGCCGCAAAATGGAAGGCGTACCGAAGTACGGT
GAGATCGTCATCGACATTAACCATGTGCCGATGCTGGCCAACGCATTCGATAAAGCCCGGGCAGCGCAAACTTCGCAGCA
GCAGGAATGGAGTACAATGCTGTTAAGTATGCTGCATGATATTCATCAGGAAAACGCCATCTATTTGATGGTGAGGAGAT
TGCGTGACTGA

Upstream 100 bases:

>100_bases
GCGAAGAACAGGTTGCACGCTGGCTGGAAGCGGAAAACGATCCGCGTCTGAACGAGATTGTCAGCCATCTGAATCATGTT
GTAGAAGAGGTGCGTATCCG

Downstream 100 bases:

>100_bases
CGTTTTACTCTGTGTTGGCAATAGCATGATGGGCGATGATGGCGCAGGTCCGCTGCTGGCGGAAAAGTGCGCCGCCGCGC
CGAAAGGTAACTGGGTGGTG

Product: processing of large subunit (HycE) of hydrogenase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYG
EIVIDINHVPMLANAFDKARAAQTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD

Sequences:

>Translated_136_residues
MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYG
EIVIDINHVPMLANAFDKARAAQTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD
>Mature_135_residues
SEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGE
IVIDINHVPMLANAFDKARAAQTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD

Specific function: Seems to be required for the conversion of a precursor form of the large subunit of hydrogenlyase (hycE) into a mature form

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli hyfJ

Homologues:

Organism=Escherichia coli, GI1789073, Length=136, Percent_Identity=100, Blast_Score=285, Evalue=7e-79,
Organism=Escherichia coli, GI87082116, Length=131, Percent_Identity=48.0916030534351, Blast_Score=122, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYCH_ECO57 (P0AEV8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F91075
- RefSeq:   NP_289267.1
- RefSeq:   NP_311601.1
- ProteinModelPortal:   P0AEV8
- EnsemblBacteria:   EBESCT00000025826
- EnsemblBacteria:   EBESCT00000058689
- GeneID:   914704
- GeneID:   961285
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4026
- KEGG:   ecs:ECs3574
- GeneTree:   EBGT00050000009393
- HOGENOM:   HBG298335
- OMA:   AMLANAF
- ProtClustDB:   PRK15084
- BioCyc:   ECOL83334:ECS3574-MONOMER
- InterPro:   IPR010005
- ProDom:   PD021295

Pfam domain/function: PF07450 HycH

EC number: NA

Molecular weight: Translated: 15465; Mature: 15334

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWI
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
ATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAAQTSQQQEWSTMLLSMLHD
HHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHQENAIYLMVRRLRD
HHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWI
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
ATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAAQTSQQQEWSTMLLSMLHD
HHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHQENAIYLMVRRLRD
HHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796