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The map label for this gene is cysN [H]

Identifier: 82545176

GI number: 82545176

Start: 2777860

End: 2779287

Strand: Direct

Name: cysN [H]

Synonym: SBO_2769

Alternate gene names: 82545176

Gene position: 2777860-2779287 (Clockwise)

Preceding gene: 82545175

Following gene: 82545177

Centisome position: 61.46

GC content: 54.76

Gene sequence:

>1428_bases
ATGAACACCGCACTTGCACAACAAATCGCCAATGAAGGCGGCGTCGAAGCCTGGATGATTGCGCAACAACATAAAAGCCT
GCTGCGTTTTCTGACCTGTGGTAGCGTCGATGACGGCAAAAGTACCCTGATTGGTCGCCTGCTGCACGATACCCGCCAAA
TCTACGAAGATCAGCTCTCATCGCTGCATAACGACAGTAAGCGTCACGGCACCCAGGGCGAAAAGCTGGATCTGGCACTG
CTGGTGGACGGCCTGCAAGCTGAGCGCGAACAGGGCATCACCATTGACGTGGCTTACCGTTATTTCTCTACCGAGAAGCG
TAAATTTATTATCGCCGACACCCCAGGGCACGAGCAGTACACTCGCAATATGGCAACTGGCGCATCGACATGTGAACTGG
CGATCTTACTGATCGATGCCCGTAAAGGCGTGCTCGATCAAACCCGTCGTCACAGTTTTATCTCCACACTGTTGGGGATC
AAACATCTGGTCGTGGCGATCAACAAAATGGATCTGGTGGATTACAGTGAAGAGACGTTCACCCATATTCGTGAAGATTA
TTTGACCTTTGCCGGGCAGCTGCCGGGTAATCTGGATATCCGCTTTGTGCCGCTCTCTGCACTGGAAGGCGACAACGTGG
CATCGCAAAGTGAAAGTATGTCGTGGTACAGCGGTCCGACACTGCTCGAAGTGCTGGAAACCGTGGAGATCCAGCGAGTG
GTGGATGCCCAGCCAATGCGCTTCCCGGTGCAGTACGTTAATCGCCCGAATCTCGATTTTCGTGGCTACGCCGGAACGCT
GGCATCCGGTCGCGTGGAAGTCGGGCAACGTGTCAAAGCGCTGCCCTCTGGTGTGGAATCAAACGTCGCGCGGATCGTGA
CTTTTGATGGTGATCGCGAAGAAGCCTTTGCCGGAGAAGCGATCACCCTGGTGCTGACGGATGAGATCGACATCAGCCGT
GGCGATCTGCTGCTGGCGGCAGACGAAGCGTTACCGGCGGTGCCGAGCGCGTCGGTGGATGTGGTATGGATGGCGGAACA
GCCGCTTTCCCCGGGCCAGAGTTACGACATCAAAATTGCCGGTAAGAAGACGCGCGCGCGTGTTGATGGCATTCGCTATC
CGGTTGATATTAATAACCTTACCCAGCGTGAAGTTGAAAACCTGCCACTGAATGGGATCGGCCTCGTGGATCTCACTTTT
GACGAGCCGCTGGTGTTAGATCGTTATCAACAAAATCCGGTGACGGGTGGGCTGATTTTTATCGATCGCCTGAGCAACGT
GACTGTTGGTGCCGGTATGGTGCATGAGCCGAAAAACGAGGCCGCCGTTGCGTCATCTGAATTCAGTGCATTCGAACTGG
AATTGAATGCCCTGGTTCGTCGCCACTTTCCGCACTGGGGCGCGCGCGATTTGCTGGGGGATAAATAA

Upstream 100 bases:

>100_bases
GAGATGCTGGTTTCTACCACCAGTGAACGTCAGGGCCGCGTGATTGACCGCGACCAGGCGGGGTCTATGGAGCTGAAAAA
ACGTCAGGGGTATTTTTAAG

Downstream 100 bases:

>100_bases
TGGCGCTGCATGACGAAAACGTCGTCTGGCATAGCCATCCGGTCACTGTGCAACAACGCGAGCTACACCACGGTCATCGT
GGTGTAGTGCTGTGGTTTAC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVPSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK

Sequences:

>Translated_475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVPSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK
>Mature_475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVPSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=442, Percent_Identity=29.185520361991, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI5729864, Length=442, Percent_Identity=29.185520361991, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI194097354, Length=293, Percent_Identity=33.7883959044369, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194018520, Length=293, Percent_Identity=33.7883959044369, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194018522, Length=293, Percent_Identity=33.7883959044369, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=27.313769751693, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI46094014, Length=293, Percent_Identity=32.7645051194539, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4503471, Length=441, Percent_Identity=26.530612244898, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI34147630, Length=331, Percent_Identity=29.0030211480363, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI157426893, Length=351, Percent_Identity=24.7863247863248, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789108, Length=475, Percent_Identity=97.6842105263158, Blast_Score=948, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=312, Percent_Identity=27.5641025641026, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789737, Length=312, Percent_Identity=27.5641025641026, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI2367247, Length=292, Percent_Identity=27.0547945205479, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115532065, Length=367, Percent_Identity=29.700272479564, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=367, Percent_Identity=29.700272479564, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32566629, Length=426, Percent_Identity=28.169014084507, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=28.9887640449438, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=28.9887640449438, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=27.6255707762557, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=241, Percent_Identity=26.9709543568465, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=345, Percent_Identity=28.4057971014493, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=393, Percent_Identity=30.0254452926209, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=27.6643990929705, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=27.6643990929705, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320377, Length=328, Percent_Identity=30.7926829268293, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324761, Length=146, Percent_Identity=34.2465753424658, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=192, Percent_Identity=32.8125, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550900, Length=438, Percent_Identity=28.310502283105, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45553807, Length=276, Percent_Identity=32.9710144927536, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553816, Length=276, Percent_Identity=32.9710144927536, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24651721, Length=276, Percent_Identity=32.9710144927536, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17864154, Length=276, Percent_Identity=32.9710144927536, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24652838, Length=443, Percent_Identity=26.1851015801354, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17137572, Length=443, Percent_Identity=26.1851015801354, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17137380, Length=315, Percent_Identity=30.1587301587302, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281363316, Length=312, Percent_Identity=29.4871794871795, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17864358, Length=312, Percent_Identity=29.4871794871795, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921738, Length=93, Percent_Identity=40.8602150537634, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52485; Mature: 52485

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
THIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKALPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEALPAVPSASVDVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYPVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHHHCCEECCEECCCHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCCEECCCCCHHEECCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
THIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKALPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEALPAVPSASVDVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYPVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHHHCCEECCEECCCHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCCEECCCCCHHEECCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA