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The map label for this gene is yfiE [H]

Identifier: 82545030

GI number: 82545030

Start: 2612785

End: 2613711

Strand: Reverse

Name: yfiE [H]

Synonym: SBO_2607

Alternate gene names: 82545030

Gene position: 2613711-2612785 (Counterclockwise)

Preceding gene: 161984880

Following gene: 82545028

Centisome position: 57.83

GC content: 48.76

Gene sequence:

>927_bases
ATGTTTTTATATTCTAGATTCGATATTTTCGATAGGTTCGGGGTTATGGATCTGCGCCGTTTTATTACGCTTAAAACCGT
GGTGGAAGAGGGTTCCTTTTTGCGAGCTTCGCAAAAATTGTGCTGTACACAATCGACGGTGACTTTTCATATTCAGCAGC
TTGAGCAGGAGTTTTCAGTCCAGTTATTTGAGAAAATTGGCCGACGGATGTGCCTTACCCGCGAAGGAAAAAAGTTGCTG
CCGCACATTTATGAACTTACCCGAGTGATGGATACGCTTCGTGAGGCTGCCAAAAAGGGGTCAGATCCGGACGGGGAACT
ACGCGTTGTTTCGGGCGAAACGTTGCTCTCTTACCGAATGCCGCAGGTGTTGCAGCGTTTTCGGCAACGCGCACCAAAAG
TGCGTTTATCGTTGCAGTCGCTGAACTGTTACGTGATCCGTGATGCGCTACTGAATGATGAGGCCGATGTCGGCGTCTTT
TATCGCGTAGGGAATGATGATGCCCTGAACCGACGAGAGTTGGGTGAACAATCGCTGGTGCTGGTGGCTTCACCGCAAAT
TGCAGATGTCGATTTTACTGAGCCGGGAAGACATAACGCCTGTAGCTTTATTATCAACGAACCGCAATGTGTCTTCCGGC
AGATATTTGAGAGCACGCTGCGTCAGCGGCGGATCACGGTGGAAAACACCATTGAGCTGTTAAGCATTGAAAGTATCAAA
CGTTGTGTCGCGGCGAATATCGGTGTCAGTTACTTGCCCCGCTTTGCGGTGGAAAAAGAGCTTGAGAGCGGAGAATTAAT
TGAATTGCCTTTTGGCGAACAGTCGCAGACCATTACGGCTATGTGCGCACACCATGCTGGAAAAGCGGTTAGCCCGGCGA
TGCACACTTTTATTCAGTGTATTGAAGAGTGTTTTTTGCCGGGATAA

Upstream 100 bases:

>100_bases
AATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTACCCGACA
CAGAACATTTAAAAAAATGA

Downstream 100 bases:

>100_bases
AAATAATGCCGGGCTTAAACCCGGCACCCAACTTGTCGACGAAGTGCGTTTTGTTCATGCCGGATGCGGCGTGAACGCCT
TATCCGGCCTACAAGATCGT

Product: transcriptional regulator LYSR-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFLYSRFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLL
PHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVF
YRVGNDDALNRRELGEQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
RCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCIEECFLPG

Sequences:

>Translated_308_residues
MFLYSRFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLL
PHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVF
YRVGNDDALNRRELGEQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
RCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCIEECFLPG
>Mature_308_residues
MFLYSRFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLL
PHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVF
YRVGNDDALNRRELGEQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
RCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCIEECFLPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=293, Percent_Identity=97.2696245733788, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1788481, Length=300, Percent_Identity=25.3333333333333, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=24.7386759581882, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786401, Length=313, Percent_Identity=26.517571884984, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=30.625, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=284, Percent_Identity=25.7042253521127, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI145693105, Length=287, Percent_Identity=22.9965156794425, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082024, Length=271, Percent_Identity=25.0922509225092, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=137, Percent_Identity=34.3065693430657, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=25.390625, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35179; Mature: 35179

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLYSRFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLSYRM
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHH
PQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLV
HHHHHHHHHHCCCEEEEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEE
LVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
EEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQC
HHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
IEECFLPG
HHHHCCCC
>Mature Secondary Structure
MFLYSRFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLSYRM
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHH
PQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLV
HHHHHHHHHHCCCEEEEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEE
LVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
EEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQC
HHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
IEECFLPG
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]