Click here to switch to the map view.

The map label for this gene is yfhK [H]

Identifier: 82545008

GI number: 82545008

Start: 2586748

End: 2588238

Strand: Reverse

Name: yfhK [H]

Synonym: SBO_2584

Alternate gene names: 82545008

Gene position: 2588238-2586748 (Counterclockwise)

Preceding gene: 82545009

Following gene: 82545007

Centisome position: 57.26

GC content: 52.52

Gene sequence:

>1491_bases
TTGTTGTATCTGAACTTCCCCTCGGTTAGCATCAGGCTATTCGCGTCTGACGAGAGTAACACCTTGAAACGCTGGCCCGT
TTTTCCCCGCTCATTACGACAACTGGTAATGCTGGCATTTTTGCTAATTCTACTACCCCTGTTGGTGCTGGCATGGCAAG
CCTGGCAAAGCCTGAATGCGCTTAGCGATCAGGCGGCGCTGGTTAACCGCACTACGCTTATTGATGCCCGGCGTAGTGAA
GCGATGACCAACGCGGCGCTGGAGATGGAGCGTAGCTACCGTCAGTATTGCGTGCTGGACGACCCTACGCTTGCGAAGGT
TTATCAAAGCCAGCGCAAGCGTTACAGTGAAATGCTCGATGCCCACGCAGGCGTACTGCCAGACGATAAACTTTACCAGG
CATTGCGTCAGGATTTGAACAATCTGGCCCAACTTCAGTGTAACAACAGTGGTCCCGATGCTGCCGCCGCCGCGCGTCTT
GAAGCCTTTGCCAGTGCCAATACCGAAATGGTGCAGGCCACGCGCACAGTGGTGTTCTCTCGTGGGCAGCAACTTCAGCG
TGAAATCGCCGAACGTGGGCAATATTTTGGTTGGCAATCGCTGGTGCTATTTCTGGTGAGTCTGGTGATGGTGCTACTTT
TCACGCGGATGATTATCGGGCCGGTGAAAAATATCGAGCGCATGATCAACCGTCTGGGGGAAGGGCGTTCTCTGGGCAAT
AGCGTTTCGTTCAGTGGACCGAGCGAGTTACGCTCGGTTGGGCAACGTATTCTTTGGTTAAGTGAGCGCCTGTCATGGCT
GGAATCCCAACGCCATCAATTTTTAAGACATTTATCTCATGAATTAAAAACGCCACTGGCGAGTATGCGCGAGGGCACTG
AATTACTGGCTGACCAGGTTGTCGGGCCGCTTACGCCAGAGCAAAAAGAGGTGGTGAGCATTCTTGATAGCAGCAGCCGC
AATTTGCAAAAACTGATCGAACAACTGCTTGATTACAACCGTAAACAGGCGGACAGTGCGGTGGAACTGGAGAATGTTGA
ATTAGCACCGCTGGTGGAGACAGTGGTTTCTGCTCATAGCCTGCCCGCACGGGCTAAAATGATGCATACCGACGTCGATC
TCAAAGCAACAGCTTGCCTGGCGGAGCCAATGCTGCTGATGAGCGTACTGGATAATCTTTACTCCAATGCGGTGCACTAC
GGGGCTGAATCCGGTAACATTTGCCTTCGCAGCAGTTCACATGGTGCGCGGGTTTATATTGATGTCATCAATACAGGCAC
GCCCATTCCGCAAGAGGAACGCGCCATGATCTTCGAACCCTTTTTTCAGGGAAGCCACCAGCGAAAAGGGGCGGTGAAGG
GCAGCGGTCTGGGATTAAGCATTGCCAGGGATTGTATTCGCCGTATGCAAGGGGAACTGTATCTGGTCGACGAGAGCGGG
CAAGACGTTTGTTTCCGCATTGAATTACCGTCGTCGAAAAACACGAAATAA

Upstream 100 bases:

>100_bases
CCGTCCAACTTCTGATATCAGCGTAGCTATATCAACCATCGGGCGAAACGTCGAGTTAGGCACCGCCTTATTCCATAACA
AAGCCGGGTAATTCCCGGCT

Downstream 100 bases:

>100_bases
TGCTAACAGCCTATCCTATCAGGCTATTTTATTTGTCAGTTCGGCTCCGGGCAGTGCTCGAAATCTTCACGTACGATATG
TACGCTCCGGTTTCTCCGCG

Product: 2-component sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSE
AMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARL
EAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
SVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSR
NLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHY
GAESGNICLRSSSHGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
QDVCFRIELPSSKNTK

Sequences:

>Translated_496_residues
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSE
AMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARL
EAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
SVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSR
NLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHY
GAESGNICLRSSSHGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
QDVCFRIELPSSKNTK
>Mature_496_residues
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSE
AMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARL
EAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
SVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSR
NLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHY
GAESGNICLRSSSHGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
QDVCFRIELPSSKNTK

Specific function: Probable member of a two-component regulatory system yfhA/yfhK. May activate yfhA by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=475, Percent_Identity=99.3684210526316, Blast_Score=970, Evalue=0.0,
Organism=Escherichia coli, GI1790861, Length=303, Percent_Identity=31.6831683168317, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693157, Length=228, Percent_Identity=29.8245614035088, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081816, Length=408, Percent_Identity=25.9803921568627, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789808, Length=291, Percent_Identity=24.3986254295533, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788713, Length=233, Percent_Identity=29.1845493562232, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789149, Length=354, Percent_Identity=23.1638418079096, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=24.1758241758242, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=24.1992882562278, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=24.6575342465753, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI48994928, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788549, Length=261, Percent_Identity=26.0536398467433, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790300, Length=232, Percent_Identity=31.0344827586207, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=25.974025974026, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787894, Length=249, Percent_Identity=26.1044176706827, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788279, Length=331, Percent_Identity=23.5649546827795, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55651; Mature: 55651

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNA
CEEEECCCEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHH
AHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFS
HHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
RGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
VGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHS
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCHHHCCCCHHHHHHHHHHHHC
LPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSSHGARVYI
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEE
DVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
EEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
QDVCFRIELPSSKNTK
CCEEEEEECCCCCCCC
>Mature Secondary Structure
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNA
CEEEECCCEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHH
AHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFS
HHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
RGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
VGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHS
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCHHHCCCCHHHHHHHHHHHHC
LPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSSHGARVYI
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEE
DVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
EEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
QDVCFRIELPSSKNTK
CCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]