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The map label for this gene is hcaR [H]

Identifier: 82544986

GI number: 82544986

Start: 2563214

End: 2564104

Strand: Reverse

Name: hcaR [H]

Synonym: SBO_2561

Alternate gene names: 82544986

Gene position: 2564104-2563214 (Counterclockwise)

Preceding gene: 82544994

Following gene: 82544985

Centisome position: 56.73

GC content: 50.06

Gene sequence:

>891_bases
ATGGAACTACGGCATTTACGCTATTTCGTCGCAGTGGCGCAGGCACTGAACTTTACCCGTGCGGCGGAAAAACTGCATAC
CTCACAGCCTTCGTTAAGCAGCCAGATCCGCGATCTTGAAAACTGTGTCGGTGTTCCGTTACTGGTGAGGGATAAGCGCA
AAGTCGCGTTGACGGCGGCGGGAGAATGTTTTCTCCAGGACGCGCTGGCAATCCTCGAACAAGCGGAAAATGCCAAATTA
CGGGCGCGGAAAACTGTTCAGGAAGACAGACAATTAACCATTGGCTTTGTGCCATCGGCGGAAGTGAATTTACTGCCAAA
AGTATTACCGATGTTTCGTCTCAGACAGCCAGACACCTTGATTGAGCTGGTGAGTTTAATCACCACGCAACAGGAGGAAA
AAATTCGCCGTGGTGAACTCGACGTCGGCTTAATGCGCCATCCTGTTTATAGCCCGGAGATTGATTATCTGGAGCTTTTT
GACGAACCATTAGTGGTTGTGTTACCGGTTGATCACCCTTTAGCACATGAAAAAGAGATCACCGCCGCGCAACTGGATGG
CGTGAATTTCGTCAGTACCGATCCGGCGTATTCCGGTTCGCTTGCGCCGATCGTTAAAGCGTGGTTTGCGCAAGAAAACA
GCCAGCCAAATATCGTCCAGGTGGCAACGAATATTCTGGTGACCATGAATCTGGTGGGCATGGGGCTGGGCGTCACTTTG
ATACCCGGTTATATGAATAATTTTAATACCGGACAGGTTGTTTTTCGTCCCATTGCCGGAAATGTGCCTTCCATTGCTTT
ACTGATGGCGTGGAAGAAAGGGGAGATGAAGCCGGCATTACGCGATTTCATCGCCATTGTGCAGGAACGTTTGGCAAGCG
TAACGGCATAA

Upstream 100 bases:

>100_bases
TCTATTTTTGTGCCATAAGGATGTTGCTGGTTGGTTGCTAATATGTGAAATAGTTAATTTCTACCAGCTGAGACGTTTTT
TCGATAACAAGGGGAAGGTG

Downstream 100 bases:

>100_bases
TTACGCATTATTTTTGCAACATGCGTCGCGGATCGCAGTTTTTTAGTGCTTGTGAACTGGCGTTACAGACATCGGGAAGC
AGGCTCGAATATTCTTATTT

Product: DNA-binding transcriptional regulator HcaR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTAAGECFLQDALAILEQAENAKL
RARKTVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVTL
IPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEMKPALRDFIAIVQERLASVTA

Sequences:

>Translated_296_residues
MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTAAGECFLQDALAILEQAENAKL
RARKTVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVTL
IPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEMKPALRDFIAIVQERLASVTA
>Mature_296_residues
MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTAAGECFLQDALAILEQAENAKL
RARKTVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVTL
IPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEMKPALRDFIAIVQERLASVTA

Specific function: Transcriptional activator of the hca operon for 3- phenylpropionic acid catabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=99.3243243243243, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1787879, Length=286, Percent_Identity=36.3636363636364, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=29.1095890410959, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI2367136, Length=286, Percent_Identity=28.3216783216783, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=31.1475409836066, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87081904, Length=182, Percent_Identity=34.6153846153846, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=30.7377049180328, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=25.9259259259259, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=25.5972696245734, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=28, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787530, Length=245, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787601, Length=284, Percent_Identity=25.7042253521127, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790262, Length=286, Percent_Identity=26.5734265734266, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788297, Length=175, Percent_Identity=33.1428571428571, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789204, Length=319, Percent_Identity=25.705329153605, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788296, Length=234, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789173, Length=176, Percent_Identity=34.0909090909091, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082132, Length=306, Percent_Identity=23.8562091503268, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32799; Mature: 32799

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTAA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCEEEEEHH
GECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDTL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHH
IELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELFDEPLVVVLPVDHPLAHEKEI
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCC
TAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVTL
HHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
IPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEMKPALRDFIAIVQERLASVTA
CCHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTAA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCEEEEEHH
GECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDTL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHH
IELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELFDEPLVVVLPVDHPLAHEKEI
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCC
TAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVTL
HHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
IPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEMKPALRDFIAIVQERLASVTA
CCHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 9603882 [H]