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The map label for this gene is hyfI

Identifier: 82544938

GI number: 82544938

Start: 2504362

End: 2505120

Strand: Direct

Name: hyfI

Synonym: SBO_2510

Alternate gene names: 82544938

Gene position: 2504362-2505120 (Clockwise)

Preceding gene: 82544937

Following gene: 82544939

Centisome position: 55.41

GC content: 56.13

Gene sequence:

>759_bases
ATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGATGAGCAAACGCAAAAGATGAAGCGGCATTTGCT
ACAGGATATCCGTCGCTCAGCCTACGTTTATCGCGTCGATTGCGGCGGCTGCAACGCCTGTGAAATCGAAATTTTTGCTG
CCATCACCCCCGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTT
ACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCCCCGATCATAAAATCTGTGTTTC
CTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGCAGCGACACCATTGTCCCCA
TTGATGTTTGGATCCCCGGCTGCCCGCCAACACCGGCCGCCACCATTCACGGTTTCGCCGTGGCGCTCGGTTTGCTGCAA
CAGAAGATTCACGCTGTGGATTATCGCGATCCCACCGGGGTGACTATGCAACCGTTGTGGCCACAGATCCCGCCATCACA
GCGTATCGCCATTGAGCGAGAAGCGCGGCGGCTGGCGGGCTATCGTCAGGGGCGAGAAATTTGCGATCGGCTCCTGCGCC
ATTTAAGCGACGATCCTACAGGAAATCGGGTTAACACCTGGTTGCGCGATGCCGACGATCCACGTCTCAATAGTATCGTT
CAGCAACTCTTTCGCGTACTCCGGGGGTTACATGACTGA

Upstream 100 bases:

>100_bases
AAATGTTGCGGGCGCAAGCGAGCGTCTGCCCGGAGTGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACCG
CTGGTGGCTAAGGAGCAGCT

Downstream 100 bases:

>100_bases
AGAGTGCGGGGAAATTGTTTTCTGGACGCTGCGAAAAAAGTTTGTCGCCAGTAGCGACGAGATGCCGGAACACCGCTCTC
AGGTAATGTATTACTCGCTG

Product: hydrogenase 4 Fe-S subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFDAERFGIKVVSSPRHADILLF
TGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIV
QQLFRVLRGLHD

Sequences:

>Translated_252_residues
MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFDAERFGIKVVSSPRHADILLF
TGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIV
QQLFRVLRGLHD
>Mature_251_residues
SPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFT
GAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQQ
KIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIVQ
QLFRVLRGLHD

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=123, Percent_Identity=41.4634146341463, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788834, Length=252, Percent_Identity=100, Blast_Score=519, Evalue=1e-149,
Organism=Escherichia coli, GI1789074, Length=251, Percent_Identity=62.1513944223108, Blast_Score=309, Evalue=1e-85,
Organism=Escherichia coli, GI1788624, Length=122, Percent_Identity=40.1639344262295, Blast_Score=91, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=122, Percent_Identity=37.7049180327869, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI18859983, Length=110, Percent_Identity=40, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24642371, Length=110, Percent_Identity=40, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24651058, Length=123, Percent_Identity=39.0243902439024, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYFI_ECOLI (P77668)

Other databases:

- EMBL:   M63654
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H65024
- RefSeq:   AP_003075.1
- RefSeq:   NP_416984.1
- ProteinModelPortal:   P77668
- SMR:   P77668
- STRING:   P77668
- EnsemblBacteria:   EBESCT00000001663
- EnsemblBacteria:   EBESCT00000016967
- GeneID:   946966
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5805
- KEGG:   eco:b2489
- EchoBASE:   EB3969
- EcoGene:   EG14217
- eggNOG:   COG3260
- GeneTree:   EBGT00050000009829
- HOGENOM:   HBG553221
- OMA:   THIEGED
- ProtClustDB:   CLSK880408
- BioCyc:   EcoCyc:MONOMER0-151
- Genevestigator:   P77668
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.-.-.- [C]

Molecular weight: Translated: 28102; Mature: 27970

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFD
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEEECCCHHC
AERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHD
HHHHCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
LYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQQKIHAVDYRDPTGVTMQPLW
HHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCC
PQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHH
QQLFRVLRGLHD
HHHHHHHHHCCC
>Mature Secondary Structure 
SPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFD
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEEECCCHHC
AERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHD
HHHHCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
LYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQQKIHAVDYRDPTGVTMQPLW
HHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCC
PQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHH
QQLFRVLRGLHD
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503