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The map label for this gene is insF1 [C]

Identifier: 82544931

GI number: 82544931

Start: 2494109

End: 2494846

Strand: Reverse

Name: insF1 [C]

Synonym: SBO_2500

Alternate gene names: 82544931

Gene position: 2494846-2494109 (Counterclockwise)

Preceding gene: 82544932

Following gene: 82544926

Centisome position: 55.2

GC content: 51.9

Gene sequence:

>738_bases
ATGTGTCAGGTATTTGGTGTATCCAGGAGCGGTTATTACAACTGGGTACAGCATGAACCCTCAGACAGAAAACAAAGTGA
TGAGCGGCTAAAACTGGAGATTAAGGTGGCACATATCCGCACTCGCGAAACATATGGAACCCGGCGGCTCCAGACGGAGC
TGGCAGAGAATGGCATCATCGTTGGTCGTGACCGACTGGCACGTCTTCGTAAGGAGCTAAGGCTACGCTGTAAGCAGAAA
CGCAAGTTCAGAGCGACTACGAACCCGAACCACAATCTGCCAGTTGCGCCAAATCTGCTGAACCAGACGTTCGCTCCTAC
AGCACCAAATCAGGTCTGGGTGGCGGACCTGACGTATGTTGCCACACAGGAGGGATGGTTGTACCTCGCTGGCATCAAAG
ATGTTTATACGTGCGAAATTGTCGGCTACGCCATGGGAGAGCGCATGACAAAAGAGCTGACAGGTAAAGCCCTGTTTATG
GCGCTCAGGAGCCAGCGCCCACTTGCCGGGCTAATCCACCACTCTGATCGAGGTTCACAGTACTGCGCATACGATTACCG
GGTCATACAGGAGCAGTCTGGTCTGAAAACATCAATGTCGCGTAAAGGTCACTGTTACGACAACGCTCCGATGGAAAGCT
TCTGGGGAACGCTGAAAAATGAGAGCCTGAGCCACTATCGTTTTAATAACCGGGATGAAGCCATCTCAGTAGTAAGCGTC
AACGGAGCACCGTATTGA

Upstream 100 bases:

>100_bases
AGCGAGATATATTAAAAAAAGCAACAGCGTATTTTGCACAGGAGTCGCTGAAAAATACGCGTTAATCGAACAATGGCGAC
AACAATTTCCCATTGAAGCG

Downstream 100 bases:

>100_bases
CGCTTATTTATTGGTGAGTGCTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAAGGCCATTCCGGCAGTACGCTCA
GGATATAGCGCAGATACGCT

Product: IS600 ORF2

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQK
RKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
ALRSQRPLAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVVSV
NGAPY

Sequences:

>Translated_245_residues
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQK
RKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
ALRSQRPLAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVVSV
NGAPY
>Mature_245_residues
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQK
RKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
ALRSQRPLAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVVSV
NGAPY

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=243, Percent_Identity=30.0411522633745, Blast_Score=120, Evalue=9e-28,
Organism=Escherichia coli, GI1788405, Length=235, Percent_Identity=41.2765957446808, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1787263, Length=235, Percent_Identity=41.2765957446808, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1786753, Length=235, Percent_Identity=41.2765957446808, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1786570, Length=235, Percent_Identity=41.2765957446808, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1786490, Length=235, Percent_Identity=41.2765957446808, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1789981, Length=237, Percent_Identity=32.0675105485232, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 28166; Mature: 28166

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGII
CCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCHHCHHHHHHHHHHCCEE
VGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYV
EEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEEE
ATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPLAGLIHHSDRGSQ
EECCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCC
YCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVVSV
EEEEHHEEEHHCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCCEEEEEEE
NGAPY
CCCCC
>Mature Secondary Structure
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGII
CCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCHHCHHHHHHHHHHCCEE
VGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYV
EEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEEE
ATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPLAGLIHHSDRGSQ
EECCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCC
YCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVVSV
EEEEHHEEEHHCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCCEEEEEEE
NGAPY
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]