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The map label for this gene is menE [H]

Identifier: 82544742

GI number: 82544742

Start: 2285818

End: 2287173

Strand: Reverse

Name: menE [H]

Synonym: SBO_2297

Alternate gene names: 82544742

Gene position: 2287173-2285818 (Counterclockwise)

Preceding gene: 82544743

Following gene: 82544741

Centisome position: 50.6

GC content: 54.87

Gene sequence:

>1356_bases
ATGATCTTCTCTGACTGGCCGTGGCGTCACTGGCGGCAAGTGCGGGGAGAAGCCATCGCCTTACGTCTTAATGACGAGCA
ACTCAACTGGCGCGAGCTTTGTGCTCGCGTCGATGAGTTAGCTACGGGCTTTGCGGCGCAGGGTGTGGTTGAGGGCAGCG
GCGTGATGTTGCGGGCGTGGAATACGCCGCAAACGCTGCTCGCCTGGCTGGCGTTACTGCAATGCGGGGCGCGGGTGCTG
CCCGTAAATCCACAGCTTCCGCAACCGTTGCTTGAGGAATTACTGCCAAATCTTACACTGCAATATGCGCTGGTGCTGGA
AGGAGAAAATACTTTCCCGGCGTTAACTTCACTGCATATTCAGATGGTTGAAGGCACACATTCAGCTCTGTGGCAACCAA
CACGTCTTTGTTCAATGACATTGACCTCCGGCTCCACCGGTTTACCGAAAGCCGCTGTCCATACTTATCAGGCCCATCTT
GCCAGTGCGCAAGGTGTGTTATCGCTGATTCCGTTTGGCGATCACGATGACTGGTTGCTCTCTTTACCGCTGTTTCACGT
CTCCGGTCAGGGAATTATGTGGCGCTGGTTATACGCTGGTGCGCGGATGACGGTGCGTGATAAACAGCCATTGGAGCAAA
TGCTGGCAGGCTGTACTCACGCTTCACTGGTGCCAACACAACTCTGGCGTTTGCTGGTTAACCGCAGTTCCGTTTCCCTG
AAAGCGGTGTTACTTGGCGGCGCGGCTATCCCGGTCGAGTTGACGGAACAGGCGCGCGAGCAGGGGATTCGTTGCTTTTG
CGGCTATGGTCTGACCGAGTTTGCCTCCACGGTATGTGCGAAAGAAGCCGACGGCCTGGCAGACGTTGGTTCGCCGCTGC
CGGGTCGGGAAGTGAAAATCGTTAATAATGAAGTGTGGCTGCGGGCTGCCAGTATGGCAGAAGGTTACTGGCGTAACGGG
CAACTGGTTTCACTGGTTAATGACGAAGGCTGGTACGCTACGCGCGATCGCGGTGAGATGCATAATGGCAAGCTGACCAT
TGTCGGACGTTTAGACAATCTGTTATTCAGTGGCGGTGAGGGTATTCAGCCGGAAGAAGTCGAGCGTGTTATTGCTGCAC
ATCCTGCGGTTTTGCAGGTGTTTATCGTTCCCGTTGCCGACAAGGAGTTTGGTCATCGGCCGGTAGCGGTGGTGGAGTAT
GACCAGCAAACCGTTGATCTTGGCGAATGGGTGAAAGATAAGCTGGCTCGTTTTCAACAACCGGTGCGCTGGCTGACTTT
ACCGCCGGAGCTGAAAAACGGCGGTATTAAAATTTCACGTCAGGCGCTAAAAGAGTGGGTGCAACGTCAGCAATAA

Upstream 100 bases:

>100_bases
CAGGGCTGGACACGCTGGATCTGATGCAGGCGCAGCAGGTACGTCGCTGGCCGGGTAGCACGCTGCCTGTCGTGGAAGTT
GATGCACTGGAGCGGTTGTT

Downstream 100 bases:

>100_bases
TTAACCCTCCGTTTCACTTCTCATTTCTGCCATTCCATTGCCGTTGATAAAGAGTTTATCTGTTAATCTGTAAATTGATG
TGAAAACTCTTAGCAAACAG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGFAAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQYALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Sequences:

>Translated_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGFAAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQYALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ
>Mature_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGFAAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQYALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=542, Percent_Identity=22.6937269372694, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI122937307, Length=262, Percent_Identity=27.8625954198473, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=96.4523281596452, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=378, Percent_Identity=24.6031746031746, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI221142682, Length=496, Percent_Identity=22.9838709677419, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=23.6686390532544, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788107, Length=352, Percent_Identity=26.1363636363636, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32563687, Length=461, Percent_Identity=26.0303687635575, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=473, Percent_Identity=25.369978858351, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=25.0501002004008, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71994694, Length=342, Percent_Identity=27.4853801169591, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=342, Percent_Identity=27.4853801169591, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=342, Percent_Identity=27.4853801169591, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=492, Percent_Identity=22.9674796747967, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=497, Percent_Identity=21.7303822937626, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=295, Percent_Identity=26.1016949152542, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25141359, Length=399, Percent_Identity=24.0601503759398, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=22.7871939736347, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50171; Mature: 50171

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGFAAQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHCCEEECCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQYALVLEGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCHHHEEE
QMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAQGVLSLIPFGDHDDWLL
EEECCCHHHHCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLLFSGGE
ECCCEEEEEHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEEEHHHHHCCCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDQQTVDLGEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGFAAQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHCCEEECCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQYALVLEGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCHHHEEE
QMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAQGVLSLIPFGDHDDWLL
EEECCCHHHHCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLLFSGGE
ECCCEEEEEHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEEEHHHHHCCCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDQQTVDLGEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]