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The map label for this gene is rlmL [H]

Identifier: 82544728

GI number: 82544728

Start: 2270369

End: 2272477

Strand: Reverse

Name: rlmL [H]

Synonym: SBO_2283

Alternate gene names: 82544728

Gene position: 2272477-2270369 (Counterclockwise)

Preceding gene: 82544730

Following gene: 82544727

Centisome position: 50.28

GC content: 54.29

Gene sequence:

>2109_bases
ATGAATTCTCTGTTTGCCAGTACGGCCCGTGGGCTGGAAGAGCTGTTAAAAACTGAACTGGAAAACCTGGGGGCCGTTGA
ATGCCAGGTGGTTCAGGGTGGGGTCCATTTCAAGGGCGACACACGGCTTGTTTACCAGAGCCTGATGTGGAGCCGCCTGG
CCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAAC
TGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTA
CGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATG
CGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCAGTATCGCTCTTGATCTGAGTGGTGATGGTTTACAT
CTGCGTGGCTATCGCGATCGTGCTGGTATTGCGCCGATCAAAGAAACCCTGGCAGCCGCTATTGTGATGCGATCCGGCTG
GCAGCCTGGAACACCGCTGCTCGATCCGATGTGTGGTTCCGGTACGTTGCTGATTGAAGCGGCGATGCTGGCGACCGATC
GCGCACCCGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAA
GCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGT
GATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAGATGTCGCGCAAC
TGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCG
GCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGC
CTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTAC
AGAAAAATTACCACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACGCCAACCGCCTGCGT
AAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGAATTGAATGTTACCGCCTGTATGACGCCGATCTGCCGGA
ATATAACGTTGCCGTTGACCGTTATTCCGACTGGGTGGTGGTGCAGGAGTATGCACCGCCAAAAACTATTGATGCTCACA
AAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAA
ACCCGTGAACGCCAGAAGGGCAAAAACCAGTACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAA
CGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGTATCGCCCGCCGTATGCTCG
GTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGTAGCGCCACCGTGCATGCGGGATTAGGCGGC
GCGCTCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGTCTGAATGGCCTAAC
CGGGCGTGCGCATCGCCTGATTCAGGCCGATTGCCTGGCGTGGCTGCGTGAGGCAAATGAACAGTTTGATCTGATCTTTA
TCGATCCGCCAACCTTCTCTAACTCAAAACGAATGGAAGATGCGTTTGATGTTCAGCGCGATCATCTGGCGCTGATGAAA
GATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGG
CCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAACGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCC
ACAACTGCTGGCTGATTACCGCAGCCTGA

Upstream 100 bases:

>100_bases
ACGTACTTGTGTACGCTCCGGTTTCTCCGCGCTGTCCATGTCCAGACTGACTGCAACAATTACGCCTACTGCGGTAGGCT
CTTAGAGTAAAAGTGACGAT

Downstream 100 bases:

>100_bases
AAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAA
CTGCATATCGAAGATAACGA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAIN
WTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEP
ALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLR
KNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ALSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMK
DLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA

Sequences:

>Translated_702_residues
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAIN
WTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEP
ALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLR
KNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ALSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMK
DLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
>Mature_702_residues
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAIN
WTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEP
ALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLR
KNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ALSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMK
DLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=702, Percent_Identity=99.5726495726496, Blast_Score=1450, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=241, Percent_Identity=29.045643153527, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 78798; Mature: 78798

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLP
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCC
LGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
CCCCEEECCCCEEEEEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
TRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLHLRGYRDRAGIAPIKETLAAA
HHCCCCCCCCCCCCCCEEEEEEEEECCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
IVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
HHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHCCCCHHEEEEHHHHHHHHCCCCC
GPYGTVLSNPPYGERLDSEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRAD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCC
KQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLRKNLKKFEKWARQEGIECYRL
CCCCCCCCCHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYSDWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
ECCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGK
CHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCH
DFLNLFSYTGSATVHAGLGGALSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLA
HHHHHHHCCCCCEEECCCCCCHHHEEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
WLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRG
HHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
FRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
EEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLP
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCC
LGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
CCCCEEECCCCEEEEEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
TRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLHLRGYRDRAGIAPIKETLAAA
HHCCCCCCCCCCCCCCEEEEEEEEECCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
IVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
HHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHCCCCHHEEEEHHHHHHHHCCCCC
GPYGTVLSNPPYGERLDSEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRAD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCC
KQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLRKNLKKFEKWARQEGIECYRL
CCCCCCCCCHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYSDWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
ECCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGK
CHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCH
DFLNLFSYTGSATVHAGLGGALSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLA
HHHHHHHCCCCCEEECCCCCCHHHEEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
WLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRG
HHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
FRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
EEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA