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The map label for this gene is rlmI [H]

Identifier: 82544709

GI number: 82544709

Start: 2250432

End: 2251535

Strand: Direct

Name: rlmI [H]

Synonym: SBO_2264

Alternate gene names: 82544709

Gene position: 2250432-2251535 (Clockwise)

Preceding gene: 161984907

Following gene: 82544710

Centisome position: 49.79

GC content: 51.99

Gene sequence:

>1104_bases
ATGGAAGGTAAAGCCAGCCTCGGTGAAACCATCGATATTGTTGATCATCAGGGAAAATGGTTAGCACGCGGCGCTTATTC
GCCAGCTTCGCAAATCCGGGCGCGCGTCTGGACGTTTGACCCGTCTGAGTCTATCGACATTGCTTTTTTTTCCCGCCGTT
TGCAACAAGCACAAAAATGGCGTGACTGGCTGGCGCAAAAAGATGGCCTCGACAGCTATCGTTTAATCGCCGGAGAATCT
GATGGCCTGCCGGGTATTACTATCGATCGTTTCGGTAATTTTCTGGTGCTGCAACTGCTGAGTGCTGGGGCAGAATATCA
GCGCGCGGCATTAGTTAGTGCCCTGCAAACGCTGTACCCGGAATGTGCGATTTACGATCGCAGCGATGTTGCGGTACGTA
AAAAAGAAGGGATGGAGCTGACCCTGGGCCTCGTCACCGGCGAGTTGCCGCCTGCCCTGCTGCCGATTGAAGAACACGGC
ATGAAGCTGCTGGTGGATATTCAGCACGGACACAAAACGGGCTACTACCTGGACCAGCGAGACAGCCGCCTGGCTACCCG
CCGCTACGTTGAAAATAAACGTGTGCTGAACTGTTTCTCCTATACCGGTGGTTTCGCCGTATCGGCACTGATGGGCGGTT
GCAGCCAGGTTGTCAGCGTTGATACCTCCCAGGAAGCGCTGGATATTGCACGGCAGAACGTTGAGCTGAACAAACTGGAT
CTGAGCAAGGCTGAGTTTGTCCGTGATGATGTCTTTAAATTGCTGCGTACTTATCGCGATCGCGGTGAAAAATTTGACGT
TATCGTGATGGACCCGCCGAAGTTTGTTGAGAATAAAAGCCAGTTGATGGGCGCGTGTCGTGGCTATAAAGATATCAACA
TGCTGGCGATTCAGTTGCTGAATGAAGGCGGTATTCTCCTGACTTTCTCCTGTTCCGGTCTGATGACCAGCGATTTATTT
CAGAAAATCATCGCGGATGCCGCAATTGATGCCGGCCGTGATGTACAATTTATAGAGCAGTTCCGTCAGGCAGCCGATCA
TCCGGTGATCGCTACCTATCCGGAAGGGCTATATCTGAAAGGGTTTGCCTGTCGCGTCATGTAA

Upstream 100 bases:

>100_bases
AGAAAAGTAAATTATGAGTGTACGTTTAGTGTTAGCCAAAGGGCGCGAAAAATCATTACTTCGTCGCCATCCGTGGGTCT
TTTCCGGGGCCGTTGCCCGC

Downstream 100 bases:

>100_bases
CTTGAAATGTGGAATTTTACCCTTATATCAAAGGTAAAGATTTCCCGGGAGGTGACTATGATTGCCAGCAAATTCGGTAT
CGGCCAGCAGGTCCGCCATT

Product: oxidoreductase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGES
DGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVAVRKKEGMELTLGLVTGELPPALLPIEEHG
MKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLF
QKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM

Sequences:

>Translated_367_residues
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGES
DGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVAVRKKEGMELTLGLVTGELPPALLPIEEHG
MKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLF
QKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
>Mature_367_residues
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGES
DGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVAVRKKEGMELTLGLVTGELPPALLPIEEHG
MKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLF
QKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=367, Percent_Identity=98.9100817438692, Blast_Score=749, Evalue=0.0,
Organism=Escherichia coli, GI1787181, Length=241, Percent_Identity=29.045643153527, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 41025; Mature: 41025

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKW
CCCCCCCCCEEEEECCCCCEEECCCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHH
RDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYP
HHHHHHCCCCCCEEEEECCCCCCCCEEHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHCC
ECAIYDRSDVAVRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQR
CCEEECCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHCCCEEEEEEECCCCCCCEECCC
DSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
CHHHHHHHHHHCCHHEEHHHCCCHHHHHHHHHCHHHHEECCCCHHHHHHHHHCCCCCEEC
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLL
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHHHHHCCCCHHHHHHHHHH
NEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLK
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEC
GFACRVM
CEEEECC
>Mature Secondary Structure
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKW
CCCCCCCCCEEEEECCCCCEEECCCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHH
RDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYP
HHHHHHCCCCCCEEEEECCCCCCCCEEHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHCC
ECAIYDRSDVAVRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQR
CCEEECCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHCCCEEEEEEECCCCCCCEECCC
DSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
CHHHHHHHHHHCCHHEEHHHCCCHHHHHHHHHCHHHHEECCCCHHHHHHHHHCCCCCEEC
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLL
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHHHHHCCCCHHHHHHHHHH
NEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLK
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEC
GFACRVM
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA