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The map label for this gene is yeiN [H]

Identifier: 82544617

GI number: 82544617

Start: 2137924

End: 2138814

Strand: Reverse

Name: yeiN [H]

Synonym: SBO_2161

Alternate gene names: 82544617

Gene position: 2138814-2137924 (Counterclockwise)

Preceding gene: 82544618

Following gene: 82544615

Centisome position: 47.32

GC content: 50.17

Gene sequence:

>891_bases
GTGCAGGACGCTTTAAAAAACAAAAAACCGGTTGTGGCGCTGGAATCGACCATTATTTCTCACGGGATGCCGTTCCCACA
AAATGCCCAGACCGCAATTGAAGTAGAAGAAACTATTCGTAAACAGGGCGCTGTACCTGCCACTATCGCCATTATTGGCG
GCGTGATGAAAGTGGGTTTAAGCAAAGAAGAATTTGAATTACTGGGTCGTGAAGGGCATAACGTGACCAAAGTTAGTCGT
CGCGATTTACCTTTTGTTGTTGCCGCCGGAAAAAATGGCGCAACCACTGTGGCTTCAACGATGATTATTGCGGCGCTTGC
CGGAATTAAAGTATTTGCCACCGGGGGAATTGGTGGTGTGCATCGCGGGGCGGAACATACCTTCGATATTTCTGCCGATT
TGCAAGAACTGGCAAATACTAATGTCACCGTTGTTTGTGCCGGGGCGAAATCTATTCTCGATTTAGGATTAACCACTGAG
TATTTAGAAACCTTCGGTGTGCCGTTAATTGGCTATCAGACTAAAGCGCTGCCTGCGTTTTTCTGCCGTACCAGCTCGTT
TGACGTCAGTATTCGTCTCGACAGCGCCAGTGAAATTGCCCGTGCAATGGCGGTGAAATGGCAAAGCGGGCTGAACGGTG
GCCTCGTGGTAGCGAACCCGATCCCGGAACAGTTTGCGATGCCGGAGGAATCTATCAATGCAGCCATAGATCAAGCCGTC
GCCGAAGCCGAAGAGCAGGGCGTTATTGGTAAAGAAAGTACACTGTTCCTGCTGGCGCGCGTTGCTGAGCTGACCGGCGG
TGACAGCCTGAAATCCAATATCCAGCTGGTATTCAACAACGCCATTCTGGCGAGTGAAATTGCCAAAGAATACCAGCGTC
TCGCGGGTTAA

Upstream 100 bases:

>100_bases
ATAACCCCGATTTATCGATTGCCAACGTTATATCGTTAGTGGAGAACGCAGAACGTCTGAATTAAAAATTTCCCCTGAAT
TATTACAAATTTCCCCGGAA

Downstream 100 bases:

>100_bases
TTTCCGACGGGCAGAAAGCCGTCGCCCGTACTCATCAAGCATACCAAAACCTGGCGCTGACGCCGGGTTTACTGGCATGA
AGGGAAAATCATTATGGATA

Product: hypothetical protein

Products: NA

Alternate protein names: PsiMP glycosidase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEFELLGREGHNVTKVSR
RDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTE
YLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAV
AEAEEQGVIGKESTLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG

Sequences:

>Translated_296_residues
MQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEFELLGREGHNVTKVSR
RDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTE
YLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAV
AEAEEQGVIGKESTLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG
>Mature_296_residues
MQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEFELLGREGHNVTKVSR
RDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTE
YLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAV
AEAEEQGVIGKESTLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG

Specific function: Catalyzes the hydrolysis of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. In vitro, can also catalyze the reverse reaction [H]

COG id: COG2313

COG function: function code Q; Uncharacterized enzyme involved in pigment biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine-5'-phosphate glycosidase family [H]

Homologues:

Organism=Escherichia coli, GI1788490, Length=296, Percent_Identity=97.2972972972973, Blast_Score=581, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI25147470, Length=289, Percent_Identity=49.8269896193772, Blast_Score=262, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24580669, Length=291, Percent_Identity=51.8900343642612, Blast_Score=278, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022830
- InterPro:   IPR007342 [H]

Pfam domain/function: PF04227 Indigoidine_A [H]

EC number: NA

Molecular weight: Translated: 31194; Mature: 31194

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGL
CCCCCCCCCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
SKEEFELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGV
CHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCC
HRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAF
CCCCCCEEECCHHHHHHCCCCEEEEECCHHHHHHHCCHHHHHHHHCCCEECCCHHHHHHH
FCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAV
EEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHCCCHHHHHHHHHHHH
AEAEEQGVIGKESTLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG
HHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHCCEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGL
CCCCCCCCCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
SKEEFELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGV
CHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCC
HRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAF
CCCCCCEEECCHHHHHHCCCCEEEEECCHHHHHHHCCHHHHHHHHCCCEECCCHHHHHHH
FCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAV
EEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHCCCHHHHHHHHHHHH
AEAEEQGVIGKESTLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG
HHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHCCEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]