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The map label for this gene is insF1 [C]

Identifier: 82544492

GI number: 82544492

Start: 1995623

End: 1995982

Strand: Direct

Name: insF1 [C]

Synonym: SBO_2025

Alternate gene names: 82544492

Gene position: 1995623-1995982 (Clockwise)

Preceding gene: 82544486

Following gene: 82544494

Centisome position: 44.15

GC content: 48.61

Gene sequence:

>360_bases
ATGGCGCTCAGGAGCCAGCGCCCACTTGCCGGGCTAATCCACCACTCTGATCGAGGTTCACAGTACTGCGCATACGATTA
CCGGGTCATACAGGAGCAGTCTGGTCTGAAAACATCAATGTCGCGTAAAGGTAACTGTTACGACAACGCTCCGATGGAAA
GCTTCTGGGGAACGCTGAAAAATGAGAGCCTGAGCCACTATCGTTTTAATAACCGGGATGAAGCCATCTCAGTAATACGG
GAATACATTGAGATTTTCTACAATCGTCAGCGTCGTCACTCTCGTCTGGGGAATATCTCCCCGGCAGCCTTCAGGGAAAA
ATATCATCAGATGACTGCTCAAAAAAAGAACAAATGGTAG

Upstream 100 bases:

>100_bases
AACGAGTATTTTTAGTCATCCTGTTTACCTCTTTCTCAGGAAGTTTAGTCTCCAGGATTCCCGGGGCGGTTCAAAAGAGC
TGACAGGTAAAGCCCTGTTT

Downstream 100 bases:

>100_bases
TGTCCGCTATTGCCAGTACACCTCATACACATCCCCTTGCTGGGTCGTATTAAAGGTTATCTGACTGGAAAGTGCCGCAA
GGAGTAATAACGCATTCATA

Product: IS600 ORF2

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 119; Mature: 118

Protein sequence:

>119_residues
MALRSQRPLAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIR
EYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW

Sequences:

>Translated_119_residues
MALRSQRPLAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIR
EYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW
>Mature_118_residues
ALRSQRPLAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIRE
YIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=108, Percent_Identity=46.2962962962963, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1787263, Length=108, Percent_Identity=46.2962962962963, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786753, Length=108, Percent_Identity=46.2962962962963, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786570, Length=108, Percent_Identity=46.2962962962963, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786490, Length=108, Percent_Identity=46.2962962962963, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1789981, Length=104, Percent_Identity=41.3461538461538, Blast_Score=78, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 14136; Mature: 14005

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRSQRPLAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLK
CCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHC
NESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALRSQRPLAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLK
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHC
NESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]