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The map label for this gene is ymfC [H]

Identifier: 82544378

GI number: 82544378

Start: 1884739

End: 1885362

Strand: Direct

Name: ymfC [H]

Synonym: SBO_1904

Alternate gene names: 82544378

Gene position: 1884739-1885362 (Clockwise)

Preceding gene: 82544376

Following gene: 82544379

Centisome position: 41.7

GC content: 51.44

Gene sequence:

>624_bases
ATGCAAAAAACTTCTTTTAGAAATCACCAGGTTAAGCGATTCAGCTCGCAACGTTCTACCAGGCGTAAACATGAAAACCA
GCCCACGCGTGTGATCCTCTTCAATAAACCCTACGATGTTCTTCCGCAGTTCACCGATGAAACCGGACGCAAAACATTAA
AAGAATTCATCCCGGTTCAGGGCGTTTATGCAGCAGGTCGCCTTGACCGCGATAGCGAAGGGTTGCTGGTGCTGACCAAT
AACGGCGCGTTGCAGGCGCGTTTAACCCAGCCGGGTAAACGCACCGGAAAAATCTATTATGTGCAGGTGGAAGGTATTCC
CACACAAGACGCACTTGAAGCCTTGCGCAATGGCGTAACCTTAAATGATGGCCCTACCCTGCCCGCAGGCGCGGAGCTGG
TTGACGAACCAGCGTGGTTATGGCCGCGGAATCCACCAATTCGTGAACGCAAAAGTATTCCCACCAGCTGGCTGAAGATC
ACCTTATATGAAGGACGTAATCGCCAGGTGCGCCGCATGACCGCCCATGTTGGTTTCCCCACGCTGCGACTGATTCGTTA
TGCGATGGGTGATTACACTTTGGATAACCTTGCCAATGGCGAATGGCGAGAAGCAACAGAATAA

Upstream 100 bases:

>100_bases
GATTTGTAATTGGCTTGTCAATACTACCCTATTGTTTGCCGTCATGAAAGATGCGCGTTATTAGGCTATAATGCGGCAAT
TCATAATCTCTGAAAATACC

Downstream 100 bases:

>100_bases
GGATGCACAATGTTTAAACCACACGTTACCGTTGCTTGCGTAGTGCACGCAGAGGGTAAATTTTTAGTCGTTGAAGAGAC
GATTAATGGTAAAGCGTTAT

Product: 23S rRNA pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILFNKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTN
NGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE

Sequences:

>Translated_207_residues
MQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILFNKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTN
NGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE
>Mature_207_residues
MQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILFNKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTN
NGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=207, Percent_Identity=97.5845410628019, Blast_Score=416, Evalue=1e-118,
Organism=Escherichia coli, GI1787524, Length=192, Percent_Identity=33.8541666666667, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788510, Length=182, Percent_Identity=34.0659340659341, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790453, Length=184, Percent_Identity=28.804347826087, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23730; Mature: 23730

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILFNKPYDVLPQFTDETGRKTLKEFIPVQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCCHHHHHHHHHHCCCC
GVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVT
CEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHCCEE
LNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKITLYEGRNRQVRRMTAHVGFP
ECCCCCCCCCHHHHCCCCEECCCCCCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCH
TLRLIRYAMGDYTLDNLANGEWREATE
HHHHHHHHHCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILFNKPYDVLPQFTDETGRKTLKEFIPVQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCCHHHHHHHHHHCCCC
GVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVT
CEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHCCEE
LNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKITLYEGRNRQVRRMTAHVGFP
ECCCCCCCCCHHHHCCCCEECCCCCCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCH
TLRLIRYAMGDYTLDNLANGEWREATE
HHHHHHHHHCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]