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The map label for this gene is dhaL

Identifier: 82544349

GI number: 82544349

Start: 1855091

End: 1855723

Strand: Direct

Name: dhaL

Synonym: SBO_1871

Alternate gene names: 82544349

Gene position: 1855091-1855723 (Clockwise)

Preceding gene: 82544348

Following gene: 82544350

Centisome position: 41.04

GC content: 53.71

Gene sequence:

>633_bases
ATGTCACTGAGCAGAACTCAAATTGTTAACTGGCTCACTCGTTGTGGCGATATTTTCAGCACCGAGAGCGAGTATCTTAC
CGGACTGGATCGCGAAATTGGCGACGCTGACCACGGGCTAAATATGAACCGAGGCTTTAGCAAAGTGGTGGAAAAACTCC
CTGCTATCGCAGATAAAGATATCGGTTTCATTCTCAAGAATACCGGTATGACGCTGCTTTCCAGCGTCGGTGGTGCCAGT
GGTCCGCTGTTCGGTACCTTCTTTATCCGCGCCGCACAGGCGACCCAGGCACGGCAAAGCCTGACACTGGAAGAGCTTTA
TCAGATGTTCCGCGATGGCGCGGACGGCGTAATCAGTCGCGGGAAAGCCGAACCTGGCGATAAAACCATGTGTGATGTGT
GGGTGCCGGTGGTGGAATCGTTACGTCAGTCCAGCGAGCAAAATCTCTCTGTTCCGGTGGCGCTCGAAGCTGCCAGTAGC
ATCGCCGAATCCGCTGCACAAAGTACGATTACGATGCAAGCCCGCAAAGGCCGCGCCAGTTATCTCGGTGAACGCAGTAT
TGGTCACCAGGATCCCGGCGCGACCTCGGTGATGTTTATGATGCAAATGTTGGCGTTAGCCGCAAAAGAGTAA

Upstream 100 bases:

>100_bases
TTCTCAATCACCTTACTGAAAGTTGATGACGAAACGCTGGCACTCTGGGACGCCCCGGTCCACACCCCGGCCCTTAACTG
GGATAAATTAGGAGAAAGCA

Downstream 100 bases:

>100_bases
GGAATTGGTGATGGTAAACCTGGTCATAGTTTCACATAGCAGCCGACTGGGAGAAGGTGTCGGTGAATTAGCCCGTCAGA
TGTTAATGAGTGATAGTTGT

Product: dihydroxyacetone kinase subunit DhaL

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNRGFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGAS
GPLFGTFFIRAAQATQARQSLTLEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE

Sequences:

>Translated_210_residues
MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNRGFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGAS
GPLFGTFFIRAAQATQARQSLTLEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE
>Mature_209_residues
SLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNRGFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASG
PLFGTFFIRAAQATQARQSLTLEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASSI
AESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE

Specific function: ADP-binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaL- ADP receives a phosphoryl group from dhaM and transmits it to dihydroxyacetone. DhaL-ADP acts also as a coactivator by binding to the sens

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaL domain

Homologues:

Organism=Homo sapiens, GI20149621, Length=182, Percent_Identity=32.967032967033, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787449, Length=210, Percent_Identity=100, Blast_Score=432, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHAL_ECOLI (P76014)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64866
- RefSeq:   AP_001824.1
- RefSeq:   NP_415717.1
- PDB:   2BTD
- PDBsum:   2BTD
- ProteinModelPortal:   P76014
- SMR:   P76014
- STRING:   P76014
- EnsemblBacteria:   EBESCT00000000403
- EnsemblBacteria:   EBESCT00000016367
- GeneID:   945748
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5186
- KEGG:   eco:b1199
- EchoBASE:   EB3659
- EcoGene:   EG13900
- eggNOG:   COG2376
- GeneTree:   EBGT00050000009463
- HOGENOM:   HBG579729
- OMA:   VWIPVIE
- ProtClustDB:   PRK10005
- BioCyc:   EcoCyc:MONOMER0-1261
- BioCyc:   MetaCyc:MONOMER0-1261
- BRENDA:   2.7.1.29
- Genevestigator:   P76014
- InterPro:   IPR004007
- InterPro:   IPR012737
- TIGRFAMs:   TIGR02365

Pfam domain/function: PF02734 Dak2; SSF101473 Dak2

EC number: 2.7.1.29

Molecular weight: Translated: 22632; Mature: 22501

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS51480 DHAL

Important sites: BINDING 121-121 BINDING 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNRGFSKVVEKLPAIADKD
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
IGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLTLEELYQMFRDGADGVISR
CHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHC
GKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASSIAESAAQSTITMQARKGRAS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
YLGERSIGHQDPGATSVMFMMQMLALAAKE
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNRGFSKVVEKLPAIADKD
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
IGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLTLEELYQMFRDGADGVISR
CHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHC
GKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASSIAESAAQSTITMQARKGRAS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
YLGERSIGHQDPGATSVMFMMQMLALAAKE
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123