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The map label for this gene is tyrS [H]

Identifier: 82543995

GI number: 82543995

Start: 1473590

End: 1474864

Strand: Direct

Name: tyrS [H]

Synonym: SBO_1497

Alternate gene names: 82543995

Gene position: 1473590-1474864 (Clockwise)

Preceding gene: 82543994

Following gene: 82543996

Centisome position: 32.6

GC content: 51.61

Gene sequence:

>1275_bases
ATGGCAAGCAGTAACTTGATTAAACAATTGCAAGAGCGGGGGCTGGTAGCCCAGGTGACGGACGAGGAAGCGTTAGCAGA
GCGACTGGCGCAAGGCCCGATCGCGCTCTATTGCGGCTTCGATCCTACCGCTGACAGCTTGCATTTGGGGCATCTTGTTC
CATTGTTATGCCTGAAACGCTTCCAACAGGCGGGCCACAAGCCGGTTGCGCTGGTAGGCGGCGCGACGGGTCTGATTGGC
GACCCGAGCTTCAAAGCTGCCGAGCGTAAGCTGAACACCGAAGAAACTGTTCAGGAGTGGGTGGACAAAATCCGTAAGCA
GGTTGCCCCGTTCCTCGATTTCGACTGTGGAGAAAACTCTGCTATCGCGGCGAACAACTATGACTGGTTCGGCAATATGA
ATGTGCTGACCTTCCTGCGCGATATTGGCAAACACTTCTCCGTTAACCAGATGATCAACAAAGAAGCGGTTAAGCAGCGT
CTCAACCGTGAAGATGAAGGGATGTCGATAACTGAGTTTTCCTACAACCTGCAGCAGGGATATGACTGGACCTGTCTGAA
CACACACTACTATGTGGAGTTGCAAATAGGTGGTTCTGACCAGAGGTGGAACATCACTTCTGAGATCGACCTGACCCGCC
GTCTGCATCAGAATCATGTGCTTGGCCTGACCGTTCCGCTGATCACTAAAGCAGATGGCACCAAATTTGGTAAAACTGAA
GGCGGCGCAGTCTGGTTGGATCCGAAGAAAACCAGCCCGTACAAATTCTACCAGTTCTGGATCAACACTGCGGATGCCGA
CGTTTACCGCTTCCTGAAGTTCTTCACCTTTATGAGCATTGAAGAGATCAACGCCCTGGAAGAAGAAGATAAAAACAGCG
GTAAAGCACCGCGCGCCCAGTATGTACTGGCGGAGCAGGTGACTCGTCTGGTTCACGGTGAAGAAGGTTTACAGGCGGCA
AAACGTATTACCGAATGCCTGTTCAGCGGTTCTTTGAGTGCGCTGAGTGAAGCGGACTTCGAACAGCTGGCGCAGGACGG
CGTACCGATGGTTGAGATGGAAAAGGGCGCAGACCTGATGCAGGCGCTGGTCGATTCTGAACTGCAACCTTCCCGTGGTC
AGGCGCGTAAAACTATCGCCTCCAATGCCATCACCATTAACGGTGAAAAACAGTCCGATCCTGAATACTTCTTTAAAGAA
GAAGATCGTCTGTTTGGTCGTTTTACCTTACTGCGTCGCGGCAAAAAGAATTACTGTCTGATTTGCTGGAAATAA

Upstream 100 bases:

>100_bases
TGGTACTCCTGATTCTGGCACTTTATTCTATGTCTCTTTCGCATCTGGCGAAAAGTCGTGTACCGGCAAAGGTGCAGTCG
TTATATACATGGAGATTTTG

Downstream 100 bases:

>100_bases
TGCATTAAGTGGAAAGGGGGAGTGAGAAATCACTCCCCCTGGTTTTTATACAGGGAACATGATGAAAAATATTCTCGCTA
TCCAGTCTCACGTTGTTTAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIG
DPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNREDEGMSITEFSYNLQQGYDWTCLNTHYYVELQIGGSDQRWNITSEIDLTRRLHQNHVLGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAA
KRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKE
EDRLFGRFTLLRRGKKNYCLICWK

Sequences:

>Translated_424_residues
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIG
DPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNREDEGMSITEFSYNLQQGYDWTCLNTHYYVELQIGGSDQRWNITSEIDLTRRLHQNHVLGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAA
KRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKE
EDRLFGRFTLLRRGKKNYCLICWK
>Mature_423_residues
ASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGD
PSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQRL
NREDEGMSITEFSYNLQQGYDWTCLNTHYYVELQIGGSDQRWNITSEIDLTRRLHQNHVLGLTVPLITKADGTKFGKTEG
GAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAK
RITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKEE
DRLFGRFTLLRRGKKNYCLICWK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=407, Percent_Identity=36.3636363636364, Blast_Score=241, Evalue=1e-63,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=96.4622641509434, Blast_Score=845, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25145802, Length=410, Percent_Identity=35.609756097561, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI25145805, Length=333, Percent_Identity=33.033033033033, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=33.7899543378995, Blast_Score=246, Evalue=4e-66,
Organism=Drosophila melanogaster, GI20130383, Length=447, Percent_Identity=36.4653243847875, Blast_Score=288, Evalue=5e-78,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47861; Mature: 47730

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQRLNREDEGMSITEFSYNLQQG
EEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECHHCCC
YDWTCLNTHYYVELQIGGSDQRWNITSEIDLTRRLHQNHVLGLTVPLITKADGTKFGKTE
CCEEEECEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
YVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKEEDRLFGRFTLLRRGKKNYCL
HHHHCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCEEE
ICWK
EEEC
>Mature Secondary Structure 
ASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQRLNREDEGMSITEFSYNLQQG
EEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECHHCCC
YDWTCLNTHYYVELQIGGSDQRWNITSEIDLTRRLHQNHVLGLTVPLITKADGTKFGKTE
CCEEEECEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
YVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKEEDRLFGRFTLLRRGKKNYCL
HHHHCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCEEE
ICWK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA