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The map label for this gene is pinE [H]

Identifier: 82543895

GI number: 82543895

Start: 1370851

End: 1371450

Strand: Reverse

Name: pinE [H]

Synonym: SBO_1391

Alternate gene names: 82543895

Gene position: 1371450-1370851 (Counterclockwise)

Preceding gene: 82543897

Following gene: 82543889

Centisome position: 30.34

GC content: 48.5

Gene sequence:

>600_bases
GTGCTGATTGGCTATGTAAGGGTATCAACAAATGACCAGAATACAGACCTGCAACAAAACGCTCTTGTTTGTGCAGGATG
TGAACAAATATTTGAAGATAAATTAAGCGGGACAAAGACAGACCGACCGGGATTAAAACGCGCTTTAAAGCGCCTTCAAA
AAGGTGACACGCTGGTTGTCTGGAAACTGGATCGCCTCGGGCGAAGCATGAAACATCTGATTTCTCTCGTCGGGGAACTA
CGGGAGCGAGGGATTAATTTTCGCAGTCTGACCGACAGCATAGACACATCTTCTCCAATGGGGCGTTTTTTCTTCCACGT
GATGGGTGCCCTGGCTGAAATGGAACGTGAATTAATTGTTGAACGTACACTGGCCGGGCTGGCGGCAGCGCGCGCAAGGG
GGCGCACAGGCGGACGTCGACCGAAGCTGACAAAAGAACAGCATGAGCAAATAGCAAGGCTAATCAAAAACGGGCACGAC
AGAAAACAACTGGCAATAATTTACGGCATTGGCACATCGACGATTTATCGTTATCACCCTGTAGGCGATATACAGACTGA
AGAAACAACCGGGCAGACTCAGGAAAATGAAAACCGCTAA

Upstream 100 bases:

>100_bases
TTCTCCCCGCTTGTGCCGAGTTCTCGTAAACCGAGGTTTTAAATAATGGCCGTTTCTGGCCTGCATGGCATGATTTGCGC
TTTTGGACGGGAGATTCAGT

Downstream 100 bases:

>100_bases
TCTGACCATTAGCGGTTTTTGTGTTAAATCAGAACAGCCCTTTAACTGAACTGGCCGCGCTGTTAAGAGATGATGTCACC
TTATCTTTGAAGCCGGACAG

Product: DNA-invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MLIGYVRVSTNDQNTDLQQNALVCAGCEQIFEDKLSGTKTDRPGLKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGEL
RERGINFRSLTDSIDTSSPMGRFFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR

Sequences:

>Translated_199_residues
MLIGYVRVSTNDQNTDLQQNALVCAGCEQIFEDKLSGTKTDRPGLKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGEL
RERGINFRSLTDSIDTSSPMGRFFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR
>Mature_199_residues
MLIGYVRVSTNDQNTDLQQNALVCAGCEQIFEDKLSGTKTDRPGLKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGEL
RERGINFRSLTDSIDTSSPMGRFFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=183, Percent_Identity=77.0491803278689, Blast_Score=293, Evalue=5e-81,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=38.2513661202186, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=38.2513661202186, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22464; Mature: 22464

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYVRVSTNDQNTDLQQNALVCAGCEQIFEDKLSGTKTDRPGLKRALKRLQKGDTLVV
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEE
WKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHDRKQLAIIYGIGTSTIYRYHP
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECC
VGDIQTEETTGQTQENENR
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLIGYVRVSTNDQNTDLQQNALVCAGCEQIFEDKLSGTKTDRPGLKRALKRLQKGDTLVV
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEE
WKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHDRKQLAIIYGIGTSTIYRYHP
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECC
VGDIQTEETTGQTQENENR
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]