Click here to switch to the map view.

The map label for this gene is yeaT [H]

Identifier: 82543798

GI number: 82543798

Start: 1279981

End: 1280925

Strand: Direct

Name: yeaT [H]

Synonym: SBO_1289

Alternate gene names: 82543798

Gene position: 1279981-1280925 (Clockwise)

Preceding gene: 82543793

Following gene: 82543801

Centisome position: 28.32

GC content: 51.96

Gene sequence:

>945_bases
GTGAAACATAAAACACGAATCATGAATAATTTACCGCTGCTGAATGATTTGCGCGTCTTTATGCTGGTGGCTCGTCGGGC
TGGTTTTGCCGCCGTGGCGGAAGAACTGGGCGTTTCACCGGCGTTCGTCAGCAAGCGCATCGCCTTGCTGGAGCAAACGC
TAAACGTGGTGTTGTTACACCGCACCACCCGCCGCGTCACCATTACCGAAGAAGGAGAGCGGATTTATGAATGGGCGCAG
CGGATTTTGCAGGATGTCGGTCAGATGATGGACGAACTTTCTGACGTGCGTCAGGTGCCACAGGGGATGCTGCGTATTAT
CAGCAGCTTTGGTTTTGGACGACAGGTGGTGGCTCCGGCGTTATCAGCGCTGGCGAAGGCGTATCCGCAACTGGAATTAC
GTTTTGATGTGGAAGACCGGCTGGTCGATTTGGTCAATGAAGGTGTCGATCTCGATATTCGCATTGGCGATGACATCGCC
CCTAATTTAATCGCCCGCAAACTGGCGACCAATTACCGCATTCTCTGCGCGTCGCCGGAATTTATTGCGCAACATGGCGC
GCCGAAACATTTAACCGATCTTTCTGCATTGCCGTGCCTGGTCATTAAAGAGCGCGATCATCCTTTTGGTGTCTGGCAAT
TACGCAATAAAGAAGGCCACCATGCGATTAAAGTCACCGGCCCACTGTCGTCTAATCATGGAGAAATTGTCCATCAGTGG
TGTCTCGATGGGCAGGGGATTGCTCTGCGTTCCTGGTGGGATGTTAGCGAAAACATTGCCAGTGGTCATTTAGTGCAAGT
GCTACCGGAATATTACCAGCCAGCGAACGTCTGGGCCGTTTATGTTTCAAGGCTGGCGACGTCAGCGAAAGTGCGGATAA
CGGTAGAATTTTTACGCCAGTATTTTGCCGAGCACTACCCGAATTTTTCACTGGAGCATGCCTGA

Upstream 100 bases:

>100_bases
TACGCATCGTTTTCATCATTGGGTTCTCACTTCCTGACCATCAAAATTGACAACATCACCATCCTATAATTGACGGAAAA
GGAATTAATCCCCGTAACTG

Downstream 100 bases:

>100_bases
TTAATGATTCAATTATCGGGTTGATATCGGGTTAAAACCTGATTTTCTCCTTTCTAAGCCGCTACAGATTGGTTAGCATA
TTCACCTTTAATCGCGCATG

Product: transcriptional regulator LYSR-type

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKHKTRIMNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLHRTTRRVTITEEGERIYEWAQ
RILQDVGQMMDELSDVRQVPQGMLRIISSFGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIA
PNLIARKLATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKVTGPLSSNHGEIVHQW
CLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQYFAEHYPNFSLEHA

Sequences:

>Translated_314_residues
MKHKTRIMNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLHRTTRRVTITEEGERIYEWAQ
RILQDVGQMMDELSDVRQVPQGMLRIISSFGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIA
PNLIARKLATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKVTGPLSSNHGEIVHQW
CLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQYFAEHYPNFSLEHA
>Mature_314_residues
MKHKTRIMNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLHRTTRRVTITEEGERIYEWAQ
RILQDVGQMMDELSDVRQVPQGMLRIISSFGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIA
PNLIARKLATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKVTGPLSSNHGEIVHQW
CLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQYFAEHYPNFSLEHA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=307, Percent_Identity=99.3485342019544, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=48.972602739726, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=33.5640138408304, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=28.0276816608997, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.6928327645051, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=29.4314381270903, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=266, Percent_Identity=24.812030075188, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1789173, Length=261, Percent_Identity=31.0344827586207, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788706, Length=270, Percent_Identity=28.1481481481481, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786448, Length=257, Percent_Identity=29.1828793774319, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI157672245, Length=169, Percent_Identity=28.9940828402367, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789492, Length=266, Percent_Identity=26.6917293233083, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786713, Length=117, Percent_Identity=37.6068376068376, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35540; Mature: 35540

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHKTRIMNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLH
CCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEE
RTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISSFGFGRQVVAPA
ECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARKLATNYRILCASPE
HHHHHHHCCCEEEEECHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHH
FIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKVTGPLSSNHGEIVHQW
HHHHCCCCCHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHH
CLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQ
HCCCCCEEEEEHHCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHH
YFAEHYPNFSLEHA
HHHHHCCCCCCCCC
>Mature Secondary Structure
MKHKTRIMNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLH
CCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEE
RTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISSFGFGRQVVAPA
ECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARKLATNYRILCASPE
HHHHHHHCCCEEEEECHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHH
FIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKVTGPLSSNHGEIVHQW
HHHHCCCCCHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHH
CLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQ
HCCCCCEEEEEHHCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHH
YFAEHYPNFSLEHA
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]