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The map label for this gene is yeaU [H]

Identifier: 82543797

GI number: 82543797

Start: 1278814

End: 1279899

Strand: Reverse

Name: yeaU [H]

Synonym: SBO_1288

Alternate gene names: 82543797

Gene position: 1279899-1278814 (Counterclockwise)

Preceding gene: 82543799

Following gene: 82543796

Centisome position: 28.32

GC content: 53.5

Gene sequence:

>1086_bases
ATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGTAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTT
ACAGGCTGCCGCTGAGCGCTGGGGCTTCGACTTGAGTTTTGAGCAAATGGGGTGGGCGAGCTGCGAGTATTACAGCCATC
ACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCG
GATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACATCAACCTGCG
CCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGG
AAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCG
GTATTTACACGTCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGCCCACGTAAGACGCTAAC
TTATGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACC
CGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGCTTCGATGTGGTG
GTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGC
CAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATA
TTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAA
GCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCAC
CACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAA

Upstream 100 bases:

>100_bases
TTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCCGTCAATTATAGGATGGTGATGTTGTCAATTTTGAT
GGTCAGGAAGTGAGAACCCA

Downstream 100 bases:

>100_bases
GGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCA
CCTTTTATTGTTTCCGTTTC

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMGWASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWP
DTVPDHISLWGSLLKFRREFDQYINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQ
AHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIILR

Sequences:

>Translated_361_residues
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMGWASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWP
DTVPDHISLWGSLLKFRREFDQYINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQ
AHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIILR
>Mature_361_residues
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMGWASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWP
DTVPDHISLWGSLLKFRREFDQYINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQ
AHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIILR

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=356, Percent_Identity=31.7415730337079, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4758582, Length=364, Percent_Identity=29.1208791208791, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI28178816, Length=363, Percent_Identity=27.8236914600551, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI28178821, Length=363, Percent_Identity=27.5482093663912, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI28178838, Length=316, Percent_Identity=30.379746835443, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178819, Length=199, Percent_Identity=28.643216080402, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788101, Length=361, Percent_Identity=98.8919667590028, Blast_Score=743, Evalue=0.0,
Organism=Escherichia coli, GI87081683, Length=371, Percent_Identity=37.466307277628, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1787381, Length=347, Percent_Identity=27.0893371757925, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17550882, Length=362, Percent_Identity=30.939226519337, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71986051, Length=362, Percent_Identity=31.4917127071823, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17505779, Length=360, Percent_Identity=28.8888888888889, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=365, Percent_Identity=27.6712328767123, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=37.5, Blast_Score=208, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6324709, Length=365, Percent_Identity=31.5068493150685, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=359, Percent_Identity=29.8050139275766, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643268, Length=364, Percent_Identity=32.4175824175824, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24643270, Length=364, Percent_Identity=32.4175824175824, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24661184, Length=361, Percent_Identity=31.5789473684211, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI161078637, Length=273, Percent_Identity=32.2344322344322, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078639, Length=273, Percent_Identity=32.2344322344322, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078635, Length=273, Percent_Identity=32.2344322344322, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078633, Length=273, Percent_Identity=32.2344322344322, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24650122, Length=273, Percent_Identity=32.2344322344322, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281362242, Length=357, Percent_Identity=29.1316526610644, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648872, Length=357, Percent_Identity=29.1316526610644, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI20130355, Length=359, Percent_Identity=23.1197771587744, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 40377; Mature: 40377

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMGWASCEYYSHHGKMMPD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCH
DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYINLRPVRLFPGVPCPLA
HHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS
CCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
RPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
CCCCEEEEEECCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHEEE
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT
EHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHH
IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIIL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
R
C
>Mature Secondary Structure
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMGWASCEYYSHHGKMMPD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCH
DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYINLRPVRLFPGVPCPLA
HHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS
CCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
RPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
CCCCEEEEEECCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHEEE
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT
EHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHH
IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIIL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]