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The map label for this gene is insF1 [C]

Identifier: 82543669

GI number: 82543669

Start: 1152523

End: 1152915

Strand: Direct

Name: insF1 [C]

Synonym: SBO_1152

Alternate gene names: 82543669

Gene position: 1152523-1152915 (Clockwise)

Preceding gene: 82543668

Following gene: 82543673

Centisome position: 25.5

GC content: 48.35

Gene sequence:

>393_bases
ATGACAAAAGAGCTGACAGGTAAAGCCCTGTTTATGGCGCTCAGGAGCCAACGCCCACCTGCCGGGCTAATCCACCACTC
TGATCGAGGTTCACAGTACTGCGCATACGATTACCGGGTCATACAGGAGCAGTCTGGTCTGAAAACATCAATGTCGCGTA
AAGGTCACTGTTACGACAACGCTCCGATGGAAAGCTTCTGGGGAACGCTGAAAAATGAGAGCCTGAGCCACTATCGTTTT
AATAACCGGGATGAAGCCATCTCAGTAATACGGGAATACATTGAGATTTTCTACAATCGTCAGCGTCGTCACTCTCGTCT
GGGGAATATCTCCCCGGCAGCCTTCAGGGAAAAATATCATCAGATGACTGCTCAAAAAAAGAACAAATGGTAG

Upstream 100 bases:

>100_bases
GGACCTGACGTATGTTGCCACACAGGAGGGATGGTTGTACCTCGCTGGCATCAAAGATGTTTATACGTGCGAAATTGTCG
GCTACGCCATGGGAGAGCGC

Downstream 100 bases:

>100_bases
TGTCCGCTATTGCCAGTACACCTCAACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCG
GAATTAACTCTTCCCACTGT

Product: IS600 ORF2

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRF
NNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW

Sequences:

>Translated_130_residues
MTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRF
NNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW
>Mature_129_residues
TKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFN
NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=118, Percent_Identity=44.9152542372881, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1787263, Length=118, Percent_Identity=44.9152542372881, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1786753, Length=118, Percent_Identity=44.9152542372881, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1786570, Length=118, Percent_Identity=44.9152542372881, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1786490, Length=118, Percent_Identity=44.9152542372881, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1789981, Length=107, Percent_Identity=40.1869158878505, Blast_Score=76, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 15364; Mature: 15232

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDN
CCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCC
APMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYH
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QMTAQKKNKW
HHHHHHCCCC
>Mature Secondary Structure 
TKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDN
CCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCC
APMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYH
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QMTAQKKNKW
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]