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The map label for this gene is recE [H]

Identifier: 82543520

GI number: 82543520

Start: 999816

End: 1001972

Strand: Direct

Name: recE [H]

Synonym: SBO_0976

Alternate gene names: 82543520

Gene position: 999816-1001972 (Clockwise)

Preceding gene: 82543519

Following gene: 82543521

Centisome position: 22.12

GC content: 49.47

Gene sequence:

>2157_bases
ATGACATTTATCGGCCCTTCCCCCTCTGTTCAGGACAAATCAGCCAGCACTGATAATACCAACATCAACGGCGAAGACAT
GACTGAGATTGAGGAGAGCATGCTTCTACCTGTCTCCGGTCAGGAACTGCCCATTCGTTGGCTTGCTCAACACGGCAGCG
AAAAACCAGTAACGCACGTTTCACGCGACGAACTCCAGGCATTACACATTGCACGGGCTGAAGAACTACCGGCTGTTACT
GCCCTGGCTATTTCGCATAAAACCAGTCTGCTCGACTCGCTGGAGATTCGCGACCTCCACAAACTGGTTCGTGACACTGA
CAAAGTTTTCCCTAATCCTGGTAATTCAGACCTGGGACTAATAACTGCTTTTTTCGAAGCATACTTGGACGCTGACTACA
CTGTTCGGGGCCTGCTGACAAAAGAGTGGATGAAAGGAAATCGTGTTTCACGCATCACCCGCACGGCTTCCGGTGCTAAT
GCTGGTGGTGGGAACAAAACCGATCGCAATCCGAATTTAGTACACACCCTCGACACACTGGATGTGGAGATTGCAGCAGC
CACACTTCCGATGGATTTTAATATTTATGAAATTCCGGGCAGCGTTTATCGTCGCGCAAAAGAAGTAGTCCTGAAAAAAG
AAAGTCCGTTCAAAGAATGGTCCGCAGCACTTCGTGCACCCCCGGGTATTCTGGACTATTCCCGCGCCGCTATTTTTGCA
CTTATCCGAAGCGCACACCCTGAATTTTATCACTACCCGGGACGCCTTCAGGGGTATATCAACGCCTATTTGACGGAAAC
TGATCACGAGAACCCCAGCAAGGAAACTCTCACAGCTACCCGGCATACGCCGGAAAAAGATATCCTGGAAGAAATTAACC
GCGAGGTGGTTACTGAGCGTGAAACAGAAGAAGAAAAACCACAACCATCTGACGCAATGGCAGGTGAACAGGCAACAACT
GAAACAATGGAACCGGATACAACTGAACATTGCCAGAACGCGCAGTCGCTGGATGCTCAGTCGCAGGTGAGTTCCGCTAA
CCAAGTAAAAGTCACCGCTGACGAAGTAAACAAAATTATGCAGGCAGCCAATATCAGCCAGCCTGACGCCGATAAGTTAC
TTGCTGTATCGCGTGGTGAATTTGTTGAGGGGATTAGCGACCCTAATGATCCGAAATGGGTCAAGGGGATCCAGACTCGC
GATTCTGTGAACCAGAACCAGCATGAATCGGAACGGAACGACCAAAAAGCGGAACAAAACAGCCCAAATGCGTTACAAAA
CGAGCCAGAAACGAAACAATCCGAACCAGTAGCGCAACAGGAACCGGAAAAAGTCTGCACCGCCTGCGGTCAGAGCGGTG
GCGGCAACTACCCTGATTGTGGCGCGGTGATGGACGACGCAACATACCAGAAAACATTCGATGAAGAGAATCAGGTTGAA
GTTCAGGAAAATGATCCGGAGGAAATGGAAGGCGCTGAACATCCACACAAGGAGAATGCTGGCAGCGCTCAGGACCACGC
CAGCGATAGTAAAACTGGCGAGACGGCAGATCCCTTAATTACGGTGAATGGTCATCACGAAATCACATCCACCAGCAGGA
CGTGTGACCATCTAATGATCGACCTTGAAACCATGGGAAAAAATCCTGATGCCCCGATCATCTCAATAGGTGCAATATTT
TTCGATCCGCAAACCGGAGATATGGGACCCGAATTTAGTAAGACTATCGATCTGGAAACTGCTGGCGGAGTCATTGATCG
GGACACCATTAAATGGTGGCTTAAGCAATCACGCGAAGCGCAATCTGCCATTATGACCGATGAAATCCCGTTAGATGATG
CACTGTTACAATTGCGGGAATTTATCGACGAAAACTCCGGTGAATTTTTTGTTCAGGTCTGGGGAAATGGAGCCAACTTC
GACAACACGATTTTGCGCCGTTCATACGAACGGCAGGGGATCCCCTGCCCGTGGCGTTACTACAACGATCGCGATGTACG
CACAATCGTTGAGCTGGGGAAAGCCATAGACTTCGATGCCAGAACGGCTATTCCATTCGAAGGTGAGCGCCATAATGCAC
TTGATGACGCTCGTTACCAGGCAAAATACGTTTCAGCTATCTGGCAAAAACTGATCCCGAGTCAGGCTGATTTTTAA

Upstream 100 bases:

>100_bases
GCCACGCAAGCACCTTAAAATCACCATCATACACTAAATCAGTAAGTTGGCAGCATTACCCTGCAACCGCTATGAAAAAG
GCGGGAAAGATGGCATCACA

Downstream 100 bases:

>100_bases
TGTTCAACCCTAATTGCCGCTAACCGTATATAGTTAGCGGCGGTTATGAGATATAGCTATGAGCAGCTTATTTTTAACCG
AAGATGAATTGCTAATATTA

Product: exodeoxyribonuclease VIII

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MTFIGPSPSVQDKSASTDNTNINGEDMTEIEESMLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVT
ALAISHKTSLLDSLEIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTVRGLLTKEWMKGNRVSRITRTASGAN
AGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAKEVVLKKESPFKEWSAALRAPPGILDYSRAAIFA
LIRSAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATT
ETMEPDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEFVEGISDPNDPKWVKGIQTR
DSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQQEPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVE
VQENDPEEMEGAEHPHKENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIF
FDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANF
DNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF

Sequences:

>Translated_718_residues
MTFIGPSPSVQDKSASTDNTNINGEDMTEIEESMLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVT
ALAISHKTSLLDSLEIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTVRGLLTKEWMKGNRVSRITRTASGAN
AGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAKEVVLKKESPFKEWSAALRAPPGILDYSRAAIFA
LIRSAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATT
ETMEPDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEFVEGISDPNDPKWVKGIQTR
DSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQQEPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVE
VQENDPEEMEGAEHPHKENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIF
FDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANF
DNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF
>Mature_717_residues
TFIGPSPSVQDKSASTDNTNINGEDMTEIEESMLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTA
LAISHKTSLLDSLEIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTVRGLLTKEWMKGNRVSRITRTASGANA
GGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAKEVVLKKESPFKEWSAALRAPPGILDYSRAAIFAL
IRSAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATTE
TMEPDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEFVEGISDPNDPKWVKGIQTRD
SVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQQEPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVEV
QENDPEEMEGAEHPHKENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFF
DPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFD
NTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=516, Percent_Identity=59.8837209302326, Blast_Score=585, Evalue=1e-168,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 79866; Mature: 79735

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFIGPSPSVQDKSASTDNTNINGEDMTEIEESMLLPVSGQELPIRWLAQHGSEKPVTHV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCC
SRDELQALHIARAEELPAVTALAISHKTSLLDSLEIRDLHKLVRDTDKVFPNPGNSDLGL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ITAFFEAYLDADYTVRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTLDTL
HHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCC
DVEIAAATLPMDFNIYEIPGSVYRRAKEVVLKKESPFKEWSAALRAPPGILDYSRAAIFA
CEEEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
LIRSAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTATRHTPEKDILEEINREVVTER
HHHHCCCCCEECCCHHCCHHHEEEECCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHHCC
ETEEEKPQPSDAMAGEQATTETMEPDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIM
HHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHCCHHHCCHHHHHCCCCEEEEEHHHHHHHH
QAANISQPDADKLLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQN
HHHCCCCCCHHHEEEECCCHHHHCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHCC
SPNALQNEPETKQSEPVAQQEPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVE
CCCHHCCCCCCCCCCCCHHCCHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCEE
VQENDPEEMEGAEHPHKENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMI
ECCCCCHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEECCCHHCHHHHEE
DLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREA
EHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHH
QSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRY
HHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCC
YNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TFIGPSPSVQDKSASTDNTNINGEDMTEIEESMLLPVSGQELPIRWLAQHGSEKPVTHV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCC
SRDELQALHIARAEELPAVTALAISHKTSLLDSLEIRDLHKLVRDTDKVFPNPGNSDLGL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ITAFFEAYLDADYTVRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTLDTL
HHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCC
DVEIAAATLPMDFNIYEIPGSVYRRAKEVVLKKESPFKEWSAALRAPPGILDYSRAAIFA
CEEEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
LIRSAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTATRHTPEKDILEEINREVVTER
HHHHCCCCCEECCCHHCCHHHEEEECCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHHCC
ETEEEKPQPSDAMAGEQATTETMEPDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIM
HHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHCCHHHCCHHHHHCCCCEEEEEHHHHHHHH
QAANISQPDADKLLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQN
HHHCCCCCCHHHEEEECCCHHHHCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHCC
SPNALQNEPETKQSEPVAQQEPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVE
CCCHHCCCCCCCCCCCCHHCCHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCEE
VQENDPEEMEGAEHPHKENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMI
ECCCCCHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEECCCHHCHHHHEE
DLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREA
EHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHH
QSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRY
HHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCC
YNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]