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The map label for this gene is wcaM [H]

Identifier: 82543421

GI number: 82543421

Start: 885109

End: 886503

Strand: Reverse

Name: wcaM [H]

Synonym: SBO_0870

Alternate gene names: 82543421

Gene position: 886503-885109 (Counterclockwise)

Preceding gene: 82543422

Following gene: 82543420

Centisome position: 19.61

GC content: 47.46

Gene sequence:

>1395_bases
ATGCCATTTAAAAAAATCTCCCGACGCACCTTCCTGACGGCAAGCTCGGCGCTTGCCTTCCTCCATACCCCTTTCGCTCG
CGCACTTCCCGCCCGACAAAGCGTTAACATTAACGACTACAACCCTCACGACTGGATCGCCTCATTTAAACAAGCCTTCA
GCGAAGGGCAAACGGTCGTCGTGCCTGCTGGATTCGTTTGTGACAATATCAACACCGGCATCTTCATTCCTCCTGGAAAA
ACGTTACACATCCTTGGAAGCCTGCGCGGCAACGGCAGAGGGCGATTTGTCTTACAGGACGGCAGCCAGGTGACAGGGGA
GGAGGGCGGCAGTATGCATAACATCACCCTGGATGTGCGTGGTTCTGACTGCACCATCAAAGGGCTGGCGATGAGCGGCT
TTGGCCCGGTAACGCAGATTTATATCGGCGGCAAAAACAAACGGGTCATGCGCAACCTGACCATCGATAACCTCACTGTC
AGCCACGCTAATTACGCCATCTTACGCCAGGGATTTCATAACCAGATAATTGGTGCCAACATCACCAACTGTAAATTTAG
TGATTTACAGGGGGACGCTATTGAATGGAACGTGGCGATTAACGACAGTGATATTTTGATCTCCGACCATGTCATCGAGC
GCATCAACTGTACCAACGGCAAAATCAACTGGGGCATTGGCATAGGCCTTGCGGGAAGCACTTACGATAATAATTACCCG
GAAGATCAGGCAGTGAAAAACTTTGTCGTGGCGAATATCACGGGATCGGATTGTCGGCAGTTGATCCATGTTGAAAATGG
CAAACATTTTGTTATCCGTAATATCAAAGCCCGCAATATCACGCCGGATTTCAGTAAGAAAGCAGGCATTGATAACGCGA
CAGTTGCTATTTACGGTTGTGACAATTTCGTGATTGATAATATTGAAATGATTAATAGCGCCGGGATGTTAATCGGCTAT
GGGGTAATTAAAGGCAAATATCTCTCGATACCGCAAAATTTCCGAGTGAATAATATTCAACTGGATAATACCCACCTTGC
TTATAAATTGCGCGGTATCCAAATCTCCGCCGGGAATGCCGTCTCCTTTGTGGCGCTGACTAACATTGAGATGAAGCGTG
CGTCGCTTGAGCTATACAATAAACCGCAACACCTTTTTATGCGTAATATCAATGTGATGCAGGAATCCTCAGTTGGACCC
GCATTGAGCATGAACTTCGACATGCGCAAAGACGTTCGCGGCGTCTTTATGGCGAAAAAAGAAACACTGCTGTCTCTTGC
AAATGTTCATGCGGTGAATGAGAAAGGACAAAGCTCCGTCGATATCGACAGGGTTAATCACCATATTGTTAATGTGGAAA
AGATTAACTTTAGATTGCCGGAACGGAGAGAGTAG

Upstream 100 bases:

>100_bases
GTTGTCAAACGTGCGCGCGAAAAAGTCGAACACGATTTTAACCAGCAGGTGATTAACCGAGAACTCGCCAGCTTGTTGCA
GGCTTTATAGAGGAGAACGT

Downstream 100 bases:

>100_bases
ATTTGCGACCATTCCTGGAAAAATGGAGCCATACTTAGGAACAATGCTACTGCAATCCACAACGAAGCGGCGTAACATCA
CAAGTAATTCAGTAATCAAT

Product: colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPFKKISRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELYNKPQHLFMRNINVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE

Sequences:

>Translated_464_residues
MPFKKISRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELYNKPQHLFMRNINVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE
>Mature_463_residues
PFKKISRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKT
LHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVS
HANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYPE
DQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYG
VIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELYNKPQHLFMRNINVMQESSVGPA
LSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788356, Length=464, Percent_Identity=97.6293103448276, Blast_Score=936, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011050 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51273; Mature: 51142

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKKISRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVV
CCCHHHCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECCEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCCEEEECHHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEEEEECCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNA
CCEEECCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELYNKPQHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECCEEEEEEHHHCCCCHHHHHHCCCEEECCCCCCEEEECCCHHHCCHHEEEEHH
ETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE
HHHHHHHHHEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PFKKISRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVV
CCHHHCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGEEGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECCEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCCEEEECHHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEEEEECCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNA
CCEEECCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELYNKPQHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECCEEEEEEHHHCCCCHHHHHHCCCEEECCCCCCEEEECCCHHHCCHHEEEEHH
ETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE
HHHHHHHHHEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]