Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 82543419

GI number: 82543419

Start: 882583

End: 883668

Strand: Reverse

Name: rfbB [H]

Synonym: SBO_0868

Alternate gene names: 82543419

Gene position: 883668-882583 (Counterclockwise)

Preceding gene: 82543420

Following gene: 82543418

Centisome position: 19.55

GC content: 43.0

Gene sequence:

>1086_bases
GTGAAAATACTTGTTACTGGTGGCGCAGGATTTATTGGTTCTGCTGTAGTTCGTCACATTATAAATAATACGCAGGATAG
TGTTGTTAATGTCGATAAATTAACGTACGCCGGAAACCTGGAATCACTTGCTGATGTTTCTGATTCTGAACGCTATGTTT
TTGAACATGCGGATATTTGCGATGCAGCTGTAATGGCACGGATTTTTGCTCAGCATCAGCCGGATGCAGTGATGCACTTG
GCTGCTGAAAGCCATGTTGACCGTTCAATTACAGGCCCTGCGGCATTTATTGAAACCAATATTGTTGGTACTTATGTCCT
TTTGGAAGCCGCTCGCAATTACTGGTCTGCTCTTGATAGCGACAAGAAAAATAGCTTCCGTCTTCATCATATTTCTACTG
ACGAAGTCTATGGTGATTTGCCTCATCCAGATGAAGTAAATAATACAGAAGAATTACCCTTATTTACTGAGACGACAGCT
TACGCGCCAAGCAGTCCTTATTCCGCATCCAAAGCATCCAGCGATCATTTAGTCCGCGCGTGGAAACGTACCTATGGTTT
ACCGACCATTGTGACTAATTGCTCTAACAATTATGGTCCTTATCATTTCCCGGAAAAATTGATTCCATTGGTTATTCTCA
ATGCTCTGGAAGGTAAAGCATTACCTATTTATGGTAAAGGGGATCAAATTCGCGACTGGCTGTATGTTGAAGATCATGCG
CGTGCGTTATATACCGTCGTAACCGAAGGTAAAGCGGGTGAAACTTATAACATTGGTGGGCACAACGAAAAGAAAAACAT
AGATGTAGTGCTCACTATTTGTGATTTGCTGGATGAGATTGTACCGAAAGAGAAATCTTATCGTGAGCAAATCACTTATA
TTGCCGATCGTCCGGGACACGATCGCCGTTATGCGATTGATGCTGAGAAGATTGGTCGCGAATTGGGATGGAAACCACAG
GAAACGTTTGAGAGCGGGATTCGGAAGACAGTGGAATGGTACCTGTCCAATACAAAATGGGTTGATAATGTGAAAAGTGG
TGCCTATCAATCGTGGATTGAAGAGAACTATGAGGGCCGCCAGTAA

Upstream 100 bases:

>100_bases
CTGTAAAGCCAGGGGCGGTAGCGTGCATTAATACCTCTATTAATCAAACTGAGAGCCGCTTATTTCACAGCATGCTCTGA
AGTAATATGGAATAAATTAA

Downstream 100 bases:

>100_bases
TGAATATCCTCCTTTTTGGCAAAACAGGGCAGGTAGGTTGGGAACTACAGCGTGCTCTGGCACCTCTGGGTAATTTGATT
GCTCTTGATGTTCACTCCAC

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDSDKKNSFRLHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYIADRPGHDRRYAIDAEKIGRELGWKPQ
ETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEENYEGRQ

Sequences:

>Translated_361_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDSDKKNSFRLHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYIADRPGHDRRYAIDAEKIGRELGWKPQ
ETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEENYEGRQ
>Mature_361_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDSDKKNSFRLHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYIADRPGHDRRYAIDAEKIGRELGWKPQ
ETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEENYEGRQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=34.7181008902077, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI42516563, Length=344, Percent_Identity=23.546511627907, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI56237023, Length=347, Percent_Identity=28.5302593659942, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI56118217, Length=347, Percent_Identity=28.5302593659942, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI189083684, Length=347, Percent_Identity=28.5302593659942, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4504031, Length=297, Percent_Identity=23.2323232323232, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=98.0609418282548, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=73.9376770538244, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI1786974, Length=356, Percent_Identity=25.2808988764045, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788366, Length=385, Percent_Identity=24.4155844155844, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=35.8208955223881, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=347, Percent_Identity=33.7175792507205, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71982035, Length=374, Percent_Identity=26.4705882352941, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=376, Percent_Identity=26.3297872340426, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=23.0769230769231, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=368, Percent_Identity=26.6304347826087, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=26.9021739130435, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=23.6686390532544, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40557; Mature: 40557

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADIC
CEEEEECCCCHHHHHHHHHHHCCCCHHEEEHHHEEECCCHHHHHCCCCCCCEEEHHHHHH
DAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDS
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKKNSFRLHHISTDEVYGDLPHPDEVNNTEELPLFTETTAYAPSSPYSASKASSDHLVRA
CCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHH
WKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHC
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYIADRPGH
CEEEEEEECCCCCCEEECCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
DRRYAIDAEKIGRELGWKPQETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEENYEGR
CCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
Q
C
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADIC
CEEEEECCCCHHHHHHHHHHHCCCCHHEEEHHHEEECCCHHHHHCCCCCCCEEEHHHHHH
DAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDS
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKKNSFRLHHISTDEVYGDLPHPDEVNNTEELPLFTETTAYAPSSPYSASKASSDHLVRA
CCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHH
WKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHC
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYIADRPGH
CEEEEEEECCCCCCEEECCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
DRRYAIDAEKIGRELGWKPQETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEENYEGR
CCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]