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The map label for this gene is rffG [C]

Identifier: 82543407

GI number: 82543407

Start: 870137

End: 870574

Strand: Direct

Name: rffG [C]

Synonym: SBO_0856

Alternate gene names: 82543407

Gene position: 870137-870574 (Clockwise)

Preceding gene: 82543403

Following gene: 82543439

Centisome position: 19.25

GC content: 39.73

Gene sequence:

>438_bases
ATGGCATTATTTAAATTCACTAAAGCCATGCTGGAAGGTAAAAGCATTGATGTTTACAACTTCGGCAAAATGAAGCGTGA
CTTTACTTACATTGATGATATTGCCGAAGCTATTATTCGCTTACAGGATGTTATTCCAGAAAAAAACCCACAGTGGGCTG
TAGAAACAGGCTCGCCTGCAACAAGTTCAGCACCATATCGTGTCTATAACATTGGTAATAGTTCGCCTGTGGAGTTGATG
GACTATATCAATGCGTTAGAAGAGGCTCTGGGTATTGAAGCCAACAAAAATATGATGCCTCTCCAACCCGGTGATGTACT
GGAAACCAGTGCTGATACAAAAGCACTGTATGACGTAATAGGATTCAAACCTGAAACGTCAGTTAAAGAAGGGGTAAAGA
ACTTTGTAGAATGGTATCGTAACTTCTATAAAGTTTGA

Upstream 100 bases:

>100_bases
TCTTTCTCAGGGAGTTTAGTCTCCAGGATTCCCGGGGCGGTTCATACCGACCACAGGGCTGCGTTTCTTTACGGTTTATG
GTCCATGGGGACGTCCGGAT

Downstream 100 bases:

>100_bases
TTTTACAAAACCATAAGAAAAGGCCCTAATTTATTAGGGCCTTTTCTTAGAATGAAACAAAATAATTAATCATTGCCAAA
CAAGTCGCGCGTATAAACTT

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELM
DYINALEEALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV

Sequences:

>Translated_145_residues
MALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELM
DYINALEEALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
>Mature_144_residues
ALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMD
YINALEEALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 16404; Mature: 16273

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKNPQWAVETGSPA
CCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
TSSAPYRVYNIGNSSPVELMDYINALEEALGIEANKNMMPLQPGDVLETSADTKALYDVI
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHH
GFKPETSVKEGVKNFVEWYRNFYKV
CCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKNPQWAVETGSPA
CHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
TSSAPYRVYNIGNSSPVELMDYINALEEALGIEANKNMMPLQPGDVLETSADTKALYDVI
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHH
GFKPETSVKEGVKNFVEWYRNFYKV
CCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]