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The map label for this gene is hisF [H]

Identifier: 82543402

GI number: 82543402

Start: 865393

End: 866169

Strand: Direct

Name: hisF [H]

Synonym: SBO_0851

Alternate gene names: 82543402

Gene position: 865393-866169 (Clockwise)

Preceding gene: 82543401

Following gene: 82543403

Centisome position: 19.15

GC content: 52.38

Gene sequence:

>777_bases
ATGCTGGCAAAACGCATAATTCCGTGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGA
AATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCCGAAGAAGGTGCAGACGAACTGGTGTTCTACGATATCACCG
CTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTCTGCGTGGCG
GGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCGCCTGCGCT
GGCGGATCCGACGTTAATTACTCGCCTGGCGGATCGTTTTGGCGTGCAGTGTATTGTGGTGGGTATTGATACCTGGTACG
ACGCTGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAGTGGGAAACGCTC
GACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAGTCGTCCTCAACATGATGAATCAGGACGGCGTGCGTAACGG
TTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTAATTGCCTCCGGTGGCGCGGGCACCATGG
AACACTTCCTCGAAGCCTTCCGTGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAAT
ATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAA

Upstream 100 bases:

>100_bases
ACATTAACGATGTTGCTGCCCTGCGTGGCACTGGCGTGCGCGGCGTAATAGTTGGTCGGGCATTATTGGAAGGTAAATTC
ACCGTGAAGGAGGCCATCGC

Downstream 100 bases:

>100_bases
CAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGC
GAAGTGTTAATGCTGGGCTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC
>Mature_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=99.6124031007752, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=26.4069264069264, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=31.1320754716981, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28441; Mature: 28441

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETLDWVQEVQKRGAGEVVLNMMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCHHHEHHHHHHHHHHHHHHHCCCHHHHHHHHH
QDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLATQGVEIRIC
HHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETLDWVQEVQKRGAGEVVLNMMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCHHHEHHHHHHHHHHHHHHHCCCHHHHHHHHH
QDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLATQGVEIRIC
HHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA