Click here to switch to the map view.

The map label for this gene is dam [C]

Identifier: 82543305

GI number: 82543305

Start: 765949

End: 766497

Strand: Direct

Name: dam [C]

Synonym: SBO_0751

Alternate gene names: 82543305

Gene position: 765949-766497 (Clockwise)

Preceding gene: 82543304

Following gene: 82543306

Centisome position: 16.95

GC content: 47.91

Gene sequence:

>549_bases
ATGAAAGCGGTATATTTCCTGTATCTCAATCGTCACGGTTACCGTGGTTTATGTCGCTATAACAAGAGCGGGCATTTCAA
CATTCCCTACGGCAATTATAAAAATCCGTATTTCCCTGAAAAAGAAATTCGCGCATTTGCAGAAAAAGCCCAGCGAGCAA
CGTTTATCTGCGCCAGCTTTGATGAAACGCTGGCGATGTTGAAGGCGGGGGATGTGGTGTATTGCGATCCACCGTATGAC
GGTACGTTTTCCGGCTATCACACTGATGGTTTCACTGAAGATGACCAGTATCACCTAGCATCCGTTCTTGAACATCGGTC
ATCAGAAGGACATCCGGTCATTGTTTCTAACAGTGACACATCCCTGATCCGTTCGCTGTATCGCAATTTTACTCACCACT
ATATCAAGGTAAAACGCAGCATCGGTGTGGCAGCTGGCGAGGGTAAATCAGCAACAGAAATCATTGCTGTTTCCGGGCCG
CGCTGCTGGGTGGGATTTGATTATTCGCGTGGCGTGGATAGTTCTGCCGTGTACGGAGTACGTGCATGA

Upstream 100 bases:

>100_bases
ACCACTTCACGCCAGAGTTTTATTTGAGATCGCAAACAGGGAGGTGGCTTATTACAACATAAGGCAGGAGTTTAATTACT
CAACTGAAATTACTGATTTC

Downstream 100 bases:

>100_bases
GCCATGCCGATATGAACAACTGCTGCGGCTTTAACGAGGCTGCCGCAGCGTTCTCATGGAACAGCCCGAAAAAGGCCATT
AACCCTTATCTGGACCCGGC

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVYCDPPYD
GTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGEGKSATEIIAVSGP
RCWVGFDYSRGVDSSAVYGVRA

Sequences:

>Translated_182_residues
MKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVYCDPPYD
GTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGEGKSATEIIAVSGP
RCWVGFDYSRGVDSSAVYGVRA
>Mature_182_residues
MKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVYCDPPYD
GTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGEGKSATEIIAVSGP
RCWVGFDYSRGVDSSAVYGVRA

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=164, Percent_Identity=48.1707317073171, Blast_Score=162, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 20478; Mature: 20478

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASF
CCEEEEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECH
DETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDT
HHHHHHHHCCCEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCH
SLIRSLYRNFTHHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGV
HHHHHHHHHHHHHHEEEHHHCCEECCCCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEE
RA
CC
>Mature Secondary Structure
MKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASF
CCEEEEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECH
DETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDT
HHHHHHHHCCCEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCH
SLIRSLYRNFTHHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGV
HHHHHHHHHHHHHHEEEHHHCCEECCCCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEE
RA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]