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The map label for this gene is insD1 [C]

Identifier: 82543286

GI number: 82543286

Start: 749530

End: 750249

Strand: Reverse

Name: insD1 [C]

Synonym: SBO_0731

Alternate gene names: 82543286

Gene position: 750249-749530 (Counterclockwise)

Preceding gene: 82543287

Following gene: 82543285

Centisome position: 16.6

GC content: 55.56

Gene sequence:

>720_bases
GTGTCGCGTGCGCAGTTGCACGTCATTCTCAGACGAACCGATGACTGGATGGATGGCCGCCGCAGTCGTCACACTGATGA
TACGGATGTGCTTCTCCGTATACACCATGTTATCGGAGAGCTGCCCACGTATGGTTATCGTCGGGTATGGGCGCTGCTTC
GCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTG
CTTGAGCGAAAACCTGCTGTACCGCCATCGAAACGGGCACATACAGGCAGAGTGGCCGTGAAAGAAAGCAATCAGCGATG
GTGCTCTGACGGGTTCGAGTTCTGCTGTGATAACGGAGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTG
AGGCACTGCACTGGGCGGTGACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCAGTGGAACGC
TGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACG
CCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCT
TCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCTAGACTGGCCCCCTGA

Upstream 100 bases:

>100_bases
TCAAAGAAGCCGTTGAATATGGACGGGCAAAAAAGTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAAGCT
TAGTCAGCCGTTGTCTCCGG

Downstream 100 bases:

>100_bases
ATCTCCAGACAACCAGTATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGTACAGATGAT
TGATGTCTTAGGGCCGGAGA

Product: IS2 ORF2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALL
LERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVER
CFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAARLAP

Sequences:

>Translated_239_residues
MSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALL
LERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVER
CFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAARLAP
>Mature_238_residues
SRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLL
ERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERC
FGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAARLAP

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=236, Percent_Identity=98.728813559322, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789424, Length=236, Percent_Identity=98.728813559322, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789224, Length=236, Percent_Identity=98.728813559322, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1788305, Length=236, Percent_Identity=98.728813559322, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1787669, Length=236, Percent_Identity=98.728813559322, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1786558, Length=236, Percent_Identity=98.728813559322, Blast_Score=484, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 27187; Mature: 27056

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELD
CCCCEEEEEEECCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
GMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGE
CCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCC
RLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERCFGNDLPSSPVEWLTDNGSC
EEEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCE
YRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAARLAP
EECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCHHCCCC
>Mature Secondary Structure 
SRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELD
CCCEEEEEEECCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
GMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGE
CCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCC
RLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERCFGNDLPSSPVEWLTDNGSC
EEEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCE
YRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAARLAP
EECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA