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The map label for this gene is yliE

Identifier: 82543280

GI number: 82543280

Start: 744222

End: 744647

Strand: Direct

Name: yliE

Synonym: SBO_0725

Alternate gene names: 82543280

Gene position: 744222-744647 (Clockwise)

Preceding gene: 82543279

Following gene: 82543281

Centisome position: 16.47

GC content: 43.19

Gene sequence:

>426_bases
ATGAAAGAAAATGGCCTGAAGGCGAACAGCCTCTGTGTTGAGATCACTGAAACCGTGATCGAGCGAATTAATGAACATTT
TTATCTCAATATTGAACAACTGCGTAAACAAGGGGTACGGATATCGATTGATGACTTTGGCACCGGTTTGTCAAACCTGA
AACGTTTTTATGAAATTAATCCAGACAGCATAAAGGTGGACTCGCAATTTACCGGCGATATTTTCGGTACTGCGGGAAAA
ATTGTGCGCATTATTTTCGATCTGGCACGCTATAACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGCGAAGACGTTGC
ACGCGAATTAATTAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCTATGCCATTCTCCGCCTGGGATA
AAAGTGGAAAATTAGTAAAAGAGTAG

Upstream 100 bases:

>100_bases
TCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATT
ACCAGCGTTTTACCACCATC

Downstream 100 bases:

>100_bases
TTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCG
GGATCTACACTCAAATGGTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGK
IVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE

Sequences:

>Translated_141_residues
MKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGK
IVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE
>Mature_141_residues
MKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGK
IVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE

Specific function: Overexpression reduces biofilm formation. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain

Homologues:

Organism=Escherichia coli, GI1787055, Length=141, Percent_Identity=100, Blast_Score=292, Evalue=6e-81,
Organism=Escherichia coli, GI87082096, Length=131, Percent_Identity=40.4580152671756, Blast_Score=88, Evalue=2e-19,
Organism=Escherichia coli, GI87081921, Length=131, Percent_Identity=34.3511450381679, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1787541, Length=124, Percent_Identity=37.0967741935484, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI87081980, Length=127, Percent_Identity=36.2204724409449, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1788849, Length=126, Percent_Identity=34.1269841269841, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI226510982, Length=135, Percent_Identity=34.0740740740741, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1790496, Length=122, Percent_Identity=34.4262295081967, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87081845, Length=122, Percent_Identity=31.9672131147541, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1786507, Length=120, Percent_Identity=31.6666666666667, Blast_Score=63, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YLIE_ECOLI (P75800)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64821
- RefSeq:   AP_001464.1
- RefSeq:   NP_415354.1
- ProteinModelPortal:   P75800
- SMR:   P75800
- STRING:   P75800
- EnsemblBacteria:   EBESCT00000004102
- EnsemblBacteria:   EBESCT00000016335
- GeneID:   945462
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0817
- KEGG:   eco:b0833
- EchoBASE:   EB3249
- EcoGene:   EG13476
- eggNOG:   COG2200
- GeneTree:   EBGT00070000032051
- HOGENOM:   HBG467594
- OMA:   GFLLHIN
- ProtClustDB:   CLSK879780
- BioCyc:   EcoCyc:G6433-MONOMER
- Genevestigator:   P75800
- InterPro:   IPR001633
- SMART:   SM00052

Pfam domain/function: PF00563 EAL

EC number: =3.1.4.52

Molecular weight: Translated: 16003; Mature: 16003

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEIN
CCCCCCCCHHHHHHHHHHHHHHCCHHEEEEHHHHHHCCCEEEEHHHCCCHHHHHHHHCCC
PDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQG
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCC
YLYQKPMPFSAWDKSGKLVKE
EEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEIN
CCCCCCCCHHHHHHHHHHHHHHCCHHEEEEHHHHHHCCCEEEEHHHCCCHHHHHHHHCCC
PDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQG
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCC
YLYQKPMPFSAWDKSGKLVKE
EEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503