Click here to switch to the map view.

The map label for this gene is moaA

Identifier: 82543228

GI number: 82543228

Start: 683066

End: 684055

Strand: Direct

Name: moaA

Synonym: SBO_0668

Alternate gene names: 82543228

Gene position: 683066-684055 (Clockwise)

Preceding gene: 82543226

Following gene: 82543229

Centisome position: 15.11

GC content: 54.14

Gene sequence:

>990_bases
ATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCG
TTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCC
GGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGTGCGCCTGACAGGAGGTGAGCCGTCTTTACGCCGCGACTTT
ACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACCACCAATGGTTACCGTCTGGAACG
CGATGTGGCGAACTGGCGCGATGCGGGACTTACTGGCATTAACGTCAGTGTCGACAGTCTGGACGCCCGCCAGTTTCACG
CTATTACCGGGCAGGATAAATTCAACCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAA
GTCAATACCGTGCTGATGCGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGGATCCAGCATCGCCCTATCCA
GCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGC
GTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCA
GATTACGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTC
CATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAAC
AGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATT
ACGCAAAACTTATCGTACATTGGCGGCTAA

Upstream 100 bases:

>100_bases
GGTCAACTGATACGGTGCTTTGGCCGTGACAATGCTCGTAAAGATTGCCACCAGGGCGAAGGAAGAAATGACTTCGCCTC
CCGTATCTGGAAAGGTGTAC

Downstream 100 bases:

>100_bases
AACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAA
TCGTCGCGGTGAAGAAGACG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDF
TDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGI
TQNLSYIGG

Sequences:

>Translated_329_residues
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDF
TDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGI
TQNLSYIGG
>Mature_328_residues
ASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFT
DIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVKV
NTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHPD
YAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGIT
QNLSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=310, Percent_Identity=39.3548387096774, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI22027536, Length=310, Percent_Identity=39.3548387096774, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=99.3920972644377, Blast_Score=680, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32566331, Length=313, Percent_Identity=36.1022364217252, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI32566329, Length=308, Percent_Identity=36.3636363636364, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17561210, Length=307, Percent_Identity=35.1791530944625, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28574490, Length=315, Percent_Identity=38.0952380952381, Blast_Score=214, Evalue=7e-56,
Organism=Drosophila melanogaster, GI28574488, Length=315, Percent_Identity=38.0952380952381, Blast_Score=214, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_ECO24 (A7ZJJ0)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461969.1
- ProteinModelPortal:   A7ZJJ0
- SMR:   A7ZJJ0
- STRING:   A7ZJJ0
- EnsemblBacteria:   EBESCT00000019070
- GeneID:   5588116
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0844
- eggNOG:   COG2896
- GeneTree:   EBGT00050000009573
- HOGENOM:   HBG529885
- OMA:   KIATRAK
- ProtClustDB:   PRK00164
- BioCyc:   ECOL331111:ECE24377A_0844-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 37305; Mature: 37174

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 17-17 BINDING 30-30 BINDING 68-68 BINDING 72-72 BINDING 99-99 BINDING 123-123 BINDING 160-160 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFA
CCCHHHHHHHHHEEEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
SLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGI
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEHHHHHHHHCCCEEE
NVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVKVNTVLMRDVNHHQLDTFLNW
EEECCCCCCHHEEEECCCHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCHHHHHHHHHH
IQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
HHCCCEEEEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCHHHHHHHHHHH
SAALREKKQTHFLHQNNTGITQNLSYIGG
HHHHHHHHHHHHHCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
ASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFA
CCHHHHHHHHHEEEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
SLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGI
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEHHHHHHHHCCCEEE
NVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVKVNTVLMRDVNHHQLDTFLNW
EEECCCCCCHHEEEECCCHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCHHHHHHHHHH
IQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
HHCCCEEEEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCHHHHHHHHHHH
SAALREKKQTHFLHQNNTGITQNLSYIGG
HHHHHHHHHHHHHCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA