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The map label for this gene is ymfT [H]

Identifier: 82542852

GI number: 82542852

Start: 270476

End: 270979

Strand: Reverse

Name: ymfT [H]

Synonym: SBO_0256

Alternate gene names: 82542852

Gene position: 270979-270476 (Counterclockwise)

Preceding gene: 82542853

Following gene: 82542850

Centisome position: 6.0

GC content: 44.05

Gene sequence:

>504_bases
GTGTTCTTGATCGAGCCATCCGATAGGCAGGTCGAAACATTTTTCAATGTGTCGTGCCATGCTGTCACCGATATTTTTAG
TAGCACCATCTCCCATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACA
CCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGCTA
AGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTGATGTGTACACTGGAGGTACATATTGTATGGAAGCGTATTGGGA
CTCTTTAACCAAAGAACAGCAGGGTGAGTTGGCCGGAAAAGTTGGCTCAACACAAGGCTACTTACGGCTGGTTTTCAATG
GCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATGCACGTCAGGTGCAATTACGAAATCTGACTTAAGA
CCGGATATCTATCCGAAAGATTAG

Upstream 100 bases:

>100_bases
CTTTCCATGCTCCGGCCTGTACCCATGATATGACAGGGACTAACGTTATTTGTTTGTTAGTGATTGAAACTTCAGGTTTT
TTTGTGATGTTCGTTGTCTG

Downstream 100 bases:

>100_bases
CAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTG
AGTGGTACGTGAAAGCTGTC

Product: hypothetical protein

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MFLIEPSDRQVETFFNVSCHAVTDIFSSTISHKPAGLGWLAINHSGKGRIPANTISQFSGVRPPDVMDCFHNEIKTLVPL
RYKYLEGSFQSCDVYTGGTYCMEAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKKLEQCTSGAITKSDLR
PDIYPKD

Sequences:

>Translated_167_residues
MFLIEPSDRQVETFFNVSCHAVTDIFSSTISHKPAGLGWLAINHSGKGRIPANTISQFSGVRPPDVMDCFHNEIKTLVPL
RYKYLEGSFQSCDVYTGGTYCMEAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKKLEQCTSGAITKSDLR
PDIYPKD
>Mature_167_residues
MFLIEPSDRQVETFFNVSCHAVTDIFSSTISHKPAGLGWLAINHSGKGRIPANTISQFSGVRPPDVMDCFHNEIKTLVPL
RYKYLEGSFQSCDVYTGGTYCMEAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKKLEQCTSGAITKSDLR
PDIYPKD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081841, Length=66, Percent_Identity=95.4545454545455, Blast_Score=129, Evalue=8e-32,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 18604; Mature: 18604

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLIEPSDRQVETFFNVSCHAVTDIFSSTISHKPAGLGWLAINHSGKGRIPANTISQFSG
CEEECCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHCC
VRPPDVMDCFHNEIKTLVPLRYKYLEGSFQSCDVYTGGTYCMEAYWDSLTKEQQGELAGK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
VGSTQGYLRLVFNGYKKASFVLAKKLEQCTSGAITKSDLRPDIYPKD
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFLIEPSDRQVETFFNVSCHAVTDIFSSTISHKPAGLGWLAINHSGKGRIPANTISQFSG
CEEECCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHCC
VRPPDVMDCFHNEIKTLVPLRYKYLEGSFQSCDVYTGGTYCMEAYWDSLTKEQQGELAGK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
VGSTQGYLRLVFNGYKKASFVLAKKLEQCTSGAITKSDLRPDIYPKD
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]