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The map label for this gene is yafE [H]

Identifier: 82542803

GI number: 82542803

Start: 217880

End: 218503

Strand: Direct

Name: yafE [H]

Synonym: SBO_0199

Alternate gene names: 82542803

Gene position: 217880-218503 (Clockwise)

Preceding gene: 82542802

Following gene: 82542806

Centisome position: 4.82

GC content: 49.68

Gene sequence:

>624_bases
ATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATGACTTATCGGC
CCACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAGATATCACCACCCGCCAGGGATATGCCGCAA
GTCTGCCATTTGCCGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCA
CTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGATGTAATGTCTCCGGGTCACCCAGTGCG
CGACATCTGGTTACAGGCGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCAGCGGTGCGTGGTTGACGT
TAATCAATGAAGCCAATCTTATAGTTGATAATTTAATTACAGATAAGTTACCGCTGGAATTTTCTTCATGGGTCGTGAGA
ATGCGTACGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTTC
CTTGCAGACTGATGGCTCTTTCACCAGTGATATCATCATGGTAGAAGCGCATAAAGCGGCATAA

Upstream 100 bases:

>100_bases
TGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCG
GCAAGCCTGATAAATAAGGT

Downstream 100 bases:

>100_bases
ATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAA
ACAACGTCAGCTTATCGCCT

Product: biotin synthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKDITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAA
LREVNRILKPGGRLIVMDVMSPGHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA

Sequences:

>Translated_207_residues
MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKDITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAA
LREVNRILKPGGRLIVMDVMSPGHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA
>Mature_206_residues
SGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKDITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAAL
REVNRILKPGGRLIVMDVMSPGHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVRM
RTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA

Specific function: Unknown

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786404, Length=207, Percent_Identity=95.6521739130435, Blast_Score=408, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: NA

Molecular weight: Translated: 22831; Mature: 22700

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKDITTRQGYAASLPFADN
CCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
AFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWLQAVEALRDT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
SHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALVDAIRIYQQSAST
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHEEEECCHHHHHHHHHHHHHCCCC
EVRTYFSLQTDGSFTSDIIMVEAHKAA
EEEEEEEEECCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKDITTRQGYAASLPFADN
CCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
AFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWLQAVEALRDT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
SHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALVDAIRIYQQSAST
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHEEEECCHHHHHHHHHHHHHCCCC
EVRTYFSLQTDGSFTSDIIMVEAHKAA
EEEEEEEEECCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1663890; 9278503 [H]