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The map label for this gene is ecpD [H]

Identifier: 82542738

GI number: 82542738

Start: 140393

End: 141133

Strand: Reverse

Name: ecpD [H]

Synonym: SBO_0126

Alternate gene names: 82542738

Gene position: 141133-140393 (Counterclockwise)

Preceding gene: 82542739

Following gene: 82542737

Centisome position: 3.12

GC content: 44.26

Gene sequence:

>741_bases
ATGTTTTTTAACACCAAACATACAACAGCTTTATGCTTCGTAACCTGTATGGCTTTTAGTTCATCGTCTATTGCGGACAT
TGTCATTTCGGGTACTCGCGTAATATATAAAAGCGATCAAAAAAGTGTCAACGTACGTCTGGAAAATAAAGGGAATAACC
CGTTGCTTGTCCAGAGTTGGTTAGATACTGGCGATGACAACGCTGAGCCTGGCAGTATTACAGTCCCTTTTACTGCCACG
CCGCCAGTATCGCGTATTGATGCCAAACGTGGGCAAACAATCAAATTAATGTACACAGCCAGCACCTCACTGCCTAAAGA
CAGAGAGAGCGTGTTCTGGTTTAACGTACTGGAAGTTCCACCAAAACCAGATGCAGAAAAGGTCGCGAATCAAAGTCTGC
TGCAACTGGCATTTCGCACACGTATAAAACTTTTCTATCGCCCGGATGGATTGAAGGGAAATCCCTCTGAAGCCCCGTTA
GCCCTGAAGTGGTTCTGGTCAGGTTCAGAAGGTAAGGCGTCATTGCGAGTGACCAATCCAACCCCTTACTACGTCTCTTT
TAGCAGTGGTGATTTAGAAGCTAGCGGTAAACGCTATCCGATTGATGTGAAAATGATTGCACCATTTAGTGATGAGGTCA
TGAAAGTCACTGGCCTTAATGGCAAAGCAAGTTCTGCAAAAGTACATTTTTACGCCATTAATGATTTTGGTGGCACCATT
GAAGGTAATGCCAGTCTGTAA

Upstream 100 bases:

>100_bases
AACACTATCAGTAAGTTGGAGTCATTACCCAGGGAATAGTGTTAATAATTTCTATTTTTATATTATTCCCTGTTTTAATT
AACTCTGTCAGGGATGGTTT

Downstream 100 bases:

>100_bases
TCAGGCAAGGATATAATTCCGCAGGAAGCATAACGTGACTATAAAACCAACTAAAAATTATCATAATCATCTGACCCGTA
TCGCTACGTTTTGCGCGCTG

Product: chaperone protein EcpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGTI
EGNASL

Sequences:

>Translated_246_residues
MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGTI
EGNASL
>Mature_246_residues
MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGTI
EGNASL

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=246, Percent_Identity=98.3739837398374, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1788428, Length=244, Percent_Identity=34.4262295081967, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789532, Length=230, Percent_Identity=33.9130434782609, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1788677, Length=211, Percent_Identity=36.9668246445498, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790771, Length=229, Percent_Identity=30.1310043668122, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786936, Length=210, Percent_Identity=30.4761904761905, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1786743, Length=239, Percent_Identity=30.9623430962343, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081806, Length=239, Percent_Identity=31.7991631799163, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787171, Length=227, Percent_Identity=28.6343612334802, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082203, Length=241, Percent_Identity=28.6307053941909, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26975; Mature: 26975

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSW
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECC
PKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLALKWFWSGSEGKASLRVTNP
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECC
TPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGTI
CCEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEEEEEECCCCEE
EGNASL
ECCCCC
>Mature Secondary Structure
MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSW
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECC
PKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLALKWFWSGSEGKASLRVTNP
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECC
TPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGTI
CCEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEEEEEECCCCEE
EGNASL
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]