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The map label for this gene is yadI

Identifier: 82542732

GI number: 82542732

Start: 132776

End: 133216

Strand: Direct

Name: yadI

Synonym: SBO_0118

Alternate gene names: 82542732

Gene position: 132776-133216 (Clockwise)

Preceding gene: 82542731

Following gene: 82542734

Centisome position: 2.94

GC content: 48.75

Gene sequence:

>441_bases
ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACT
TCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATG
AAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTACCGCGTGGCTTCATTATTAAGC
CATAAACACTCCCGTTGTGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCAT
GACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAA
ATCCGCCTTTTGTCCTCAAACATAATTTGTATGAGTATTAA

Upstream 100 bases:

>100_bases
ATTTCCTCTCCTCTGGATTCGGGGGAGAGGAGTTTTGACGGCTATCACCCTTTATCAACAATGGTCAGGATAGACTGATT
TTCGGCTAAGGAGGAAGGCG

Downstream 100 bases:

>100_bases
CCCGCGATTCTGATGGCGCTTTTGCTACAATACAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTATAAACAAGCTGT
TATTCATCCTGCTGATGCTG

Product: PTS enzyme II B component

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLS
HKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY

Sequences:

>Translated_146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLS
HKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY
>Mature_146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLS
HKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain

Homologues:

Organism=Escherichia coli, GI1786321, Length=146, Percent_Identity=100, Blast_Score=306, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YADI_ECOLI (P36881)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64736
- RefSeq:   AP_000790.1
- RefSeq:   NP_414671.1
- ProteinModelPortal:   P36881
- SMR:   P36881
- STRING:   P36881
- EnsemblBacteria:   EBESCT00000003637
- EnsemblBacteria:   EBESCT00000017562
- GeneID:   947397
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0125
- KEGG:   eco:b0129
- EchoBASE:   EB2227
- EcoGene:   EG12322
- eggNOG:   COG2893
- GeneTree:   EBGT00050000009723
- HOGENOM:   HBG734163
- OMA:   LPLRESM
- ProtClustDB:   CLSK879563
- BioCyc:   EcoCyc:AGAX-MONOMER
- Genevestigator:   P36881
- GO:   GO:0005737
- InterPro:   IPR004701

Pfam domain/function: PF03610 EIIA-man; SSF53062 PTS_EIIA_fruc

EC number: 2.7.1.69

Molecular weight: Translated: 16540; Mature: 16540

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: ACT_SITE 9-9

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEG
CCCEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
VIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELG
EEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
APEVSSLWHQQQKNPPFVLKHNLYEY
CCHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEG
CCCEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
VIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELG
EEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
APEVSSLWHQQQKNPPFVLKHNLYEY
CCHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503