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The map label for this gene is aceF [H]

Identifier: 82542718

GI number: 82542718

Start: 114355

End: 115944

Strand: Direct

Name: aceF [H]

Synonym: SBO_0104

Alternate gene names: 82542718

Gene position: 114355-115944 (Clockwise)

Preceding gene: 82542717

Following gene: 82542719

Centisome position: 2.53

GC content: 54.34

Gene sequence:

>1590_bases
ATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAA
AGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCG
TTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCA
GCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCCGCGGCTGCGGCGGCAAAAGA
CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTG
AACAGTCGCTGATCACCGTAGAAGGCGATAAAGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATC
AAAGTGAACGTGGGTGACAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGTTGAAGGCGCAGCGCCTGCGGCAGC
TCCTGCGAAACAGGAAGCGGCAGCGCCGGCACCGGCAGCAAAAGCTGAAGCCCCGGCAGCAGCACCAGCTGCGAAAGCGG
AAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGT
GTTAACCTGGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGC
TATCAAACGTGCAGAAGCAGCTCCGGCGGCGACTGGCGGCGGTATCCCAGGCATGCTGCCGTGGCCGAAGGTGGACTTCA
GCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGTATCCAGAAAATTTCTGGTGCTAACCTGAGCCGTAACTGGGTG
ATGATCCCGCATGTTACCCACTTCGACAAAACCGATATCACCGAGCTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGC
GGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGC
CTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACTCTGAAGAAATACATCAACATCGGTGTGGCGGTGGAT
ACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGAC
TATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCATCGGCG
GCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAG
CCGGTGTGGAATGGTAAAGAGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGG
TGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAA

Upstream 100 bases:

>100_bases
GTGGCGAAATCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTG
GCGTAAGAGGTAAAAGAATA

Downstream 100 bases:

>100_bases
GTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCG
GTTGCCGTTTGTTGTTTAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGA
ADAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
KVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFG
VNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWV
MIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVD
TPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAME
PVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM

Sequences:

>Translated_529_residues
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGA
ADAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
KVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFG
VNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWV
MIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVD
TPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAME
PVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM
>Mature_528_residues
AIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAA
DAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEIK
VNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGV
NLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVM
IPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDT
PNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEP
VWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=30.045871559633, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI31711992, Length=418, Percent_Identity=30.8612440191388, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=442, Percent_Identity=27.6018099547511, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI203098753, Length=442, Percent_Identity=27.6018099547511, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI19923748, Length=205, Percent_Identity=33.6585365853659, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI260898739, Length=170, Percent_Identity=35.8823529411765, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786305, Length=526, Percent_Identity=92.0152091254753, Blast_Score=880, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=30.9133489461358, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=407, Percent_Identity=30.958230958231, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17560088, Length=413, Percent_Identity=29.0556900726392, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=207, Percent_Identity=36.231884057971, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=29.0322580645161, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=414, Percent_Identity=27.536231884058, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=429, Percent_Identity=28.6713286713287, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=31.1004784688995, Blast_Score=178, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=29.7872340425532, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=33.953488372093, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=29.7872340425532, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56116; Mature: 55985

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVS
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEV
CCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHH
TEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEIKVNVGDKVKTGSLIMIFEVE
HHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEECCCCCEECCCEEEEEEEC
GAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFG
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCCCEEEHHHHHHHHHHHHC
VNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIE
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCC
EVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVV
EECCCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHH
FIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIE
HHHHHHHHHHHHCCCCCCCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHH
LSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAME
HHHHHHHHHHHCCCCCCEECCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCC
PVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM
CCCCCHHHHHHEEEEEEECCCCEEEECCCCCEEEEEEHHHHHHHHHHHC
>Mature Secondary Structure 
AIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVS
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEV
CCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHH
TEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEIKVNVGDKVKTGSLIMIFEVE
HHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEECCCCCEECCCEEEEEEEC
GAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFG
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCCCEEEHHHHHHHHHHHHC
VNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIE
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCC
EVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVV
EECCCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHH
FIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIE
HHHHHHHHHHHHCCCCCCCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHH
LSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAME
HHHHHHHHHHHCCCCCCEECCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCC
PVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM
CCCCCHHHHHHEEEEEEECCCCEEEECCCCCEEEEEEHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]