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The map label for this gene is fruR [H]

Identifier: 82542684

GI number: 82542684

Start: 76031

End: 77035

Strand: Direct

Name: fruR [H]

Synonym: SBO_0068

Alternate gene names: 82542684

Gene position: 76031-77035 (Clockwise)

Preceding gene: 82542682

Following gene: 82542685

Centisome position: 1.68

GC content: 54.93

Gene sequence:

>1005_bases
GTGAAACTGGATGAAATCGCTCGGCTGGCGGGAGTGTCGCGGACCACTGCAAGCTATGTTATTAACGGCAAAGCGAAGCA
ATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAG
CTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCT
AACTATCTTGAGCGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAAT
GCGCTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCACCTGAGCATCCTTTTT
ATCAACGCTGGGCTAACGCCCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACATTTCACCAGCGTGGTT
GGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGCGC
GCTGCCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATT
TCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCTGATGCCGCAG
GCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGGGTGATGGATGTCACGCTACGTCGCGATGGTAAACTGCCTTCTGA
CCTGGCAATTGCCACCTTTGGCGATAACGAACTGCTCGACTTCTTACAGTGCCCGGTGCTGGCAGTGGCTCAACGTCACC
GCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGC
ATTAAACGTAATCTCTATCGCCGCGGTGTGCTCAGCCGTAGCTAA

Upstream 100 bases:

>100_bases
AAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTAC
ATTTTACGCAAGGGGCAATT

Downstream 100 bases:

>100_bases
GCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTA
AATAAGACTATTCCTGACTA

Product: DNA-binding transcriptional regulator FruR

Products: NA

Alternate protein names: Catabolite repressor/activator [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHLMPQ
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTR
IKRNLYRRGVLSRS

Sequences:

>Translated_334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHLMPQ
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTR
IKRNLYRRGVLSRS
>Mature_334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHLMPQ
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTR
IKRNLYRRGVLSRS

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=334, Percent_Identity=99.4011976047904, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1790194, Length=316, Percent_Identity=26.8987341772152, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787580, Length=289, Percent_Identity=23.1833910034602, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=22.2222222222222, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790369, Length=312, Percent_Identity=23.0769230769231, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790715, Length=283, Percent_Identity=25.4416961130742, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789068, Length=304, Percent_Identity=24.6710526315789, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788474, Length=279, Percent_Identity=25.089605734767, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789202, Length=128, Percent_Identity=32.03125, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37972; Mature: 37972

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGR
CCHHHHHHHHCCCHHHHHHEECCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHE
IIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVVGADQDDAEMLAEELRKFPAE
EEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
TVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHLMPQ
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHH
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGR
CCHHHHHHHHCCCHHHHHHEECCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHE
IIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVVGADQDDAEMLAEELRKFPAE
EEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
TVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHLMPQ
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHH
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]