Click here to switch to the map view.

The map label for this gene is purU

Identifier: 78780180

GI number: 78780180

Start: 1690744

End: 1691598

Strand: Reverse

Name: purU

Synonym: PMT9312_1795

Alternate gene names: 78780180

Gene position: 1691598-1690744 (Counterclockwise)

Preceding gene: 78780182

Following gene: 78780177

Centisome position: 98.97

GC content: 30.41

Gene sequence:

>855_bases
TTGGAACATCCTTCAATTATATTCAAAATTGTTTGTCCCGATCGTCCTGGCCTTGTAAGTTTACTTACAAGTTGGATTTC
AAATTACGGTGGAAACATAAAACATTCTGATCATCACACAGATCAAGATGCGGGTTTGTTTCTTAGTCGAATTGAATGGA
ATAGTAAAAATGCATCTTTAAATAGATATGAAATCTATAAAGAATTTGAAAAAATTGCAGATGAAGTAAATGGAAAATTT
AACGTAAATTATTCTGATGAAATTCCAAATGTTGCTATTTTCGTGAGTAAGCAAAATCATTGTTTGATTGATTTGCTTTG
GCGAGTAAGAAATGGTGAACTCAAAATGAAAGTGCCGTTAATAATTTCTAATCATTCTGATCTTGAAAATATTGCAAATG
ATTTTAATGCAAAATTTGTCTATATTGATACCTTTAATACTGACAAATCTATTGTTGAAGATCAATTTTTAAATTTATTA
AAAGAATATGAAATTGATCTTGTTGTATTAGCCAAATATATGCAAATTTTGAGTGACTCTTTTTTAAAAAAGTTTTCTTC
AATAATAAATATTCATCATTCTTTCTTACCTGCATTTAAGGGCGGGCAACCATATCATCGAGCATGGAAGAGAGGTGTTA
AATTAATCGGTGCTACTGCTCACTATGTTACTGAAGATCTTGATGAAGGCCCGATAATAGAGCAATGCACAGTTAATGTA
AGTCATAGGGATGAAGTTGATGATTTGATAAGAAAAGGGAGAGATATTGAAAGAATAGCTTTAGCAAGAGCGGTTAGATT
ACATCTAAATCATCAAGTATTTGTTTATAACAGCAAAACTGCTGTTTTTGATTGA

Upstream 100 bases:

>100_bases
ATCCAATAATGACTATGTGTGCTTTTTGAAAATTTTCTTTTAAGCTTTTCATTGATATATTATATATACTTGCTTATTTG
ACTTAATAGTTAAAGATTTT

Downstream 100 bases:

>100_bases
AGATAGTTTCTTAGTCTTCTAATTCTATTTGTATTTGTCTTTCATAAATTGATTCTTCATTAAAAGCTCTTGCTATCAAG
AAACTGGCAATGCAGCTGAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWNSKNASLNRYEIYKEFEKIADEVNGKF
NVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGELKMKVPLIISNHSDLENIANDFNAKFVYIDTFNTDKSIVEDQFLNLL
KEYEIDLVVLAKYMQILSDSFLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIEQCTVNV
SHRDEVDDLIRKGRDIERIALARAVRLHLNHQVFVYNSKTAVFD

Sequences:

>Translated_284_residues
MEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWNSKNASLNRYEIYKEFEKIADEVNGKF
NVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGELKMKVPLIISNHSDLENIANDFNAKFVYIDTFNTDKSIVEDQFLNLL
KEYEIDLVVLAKYMQILSDSFLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIEQCTVNV
SHRDEVDDLIRKGRDIERIALARAVRLHLNHQVFVYNSKTAVFD
>Mature_284_residues
MEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWNSKNASLNRYEIYKEFEKIADEVNGKF
NVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGELKMKVPLIISNHSDLENIANDFNAKFVYIDTFNTDKSIVEDQFLNLL
KEYEIDLVVLAKYMQILSDSFLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIEQCTVNV
SHRDEVDDLIRKGRDIERIALARAVRLHLNHQVFVYNSKTAVFD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=204, Percent_Identity=32.3529411764706, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI209869995, Length=204, Percent_Identity=32.3529411764706, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI209869993, Length=204, Percent_Identity=32.3529411764706, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1787483, Length=282, Percent_Identity=35.4609929078014, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI1788846, Length=167, Percent_Identity=28.1437125748503, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=165, Percent_Identity=33.9393939393939, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=27.2251308900524, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32745; Mature: 32745

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWNSKNASL
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCC
NRYEIYKEFEKIADEVNGKFNVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGELKMKVPL
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEE
IISNHSDLENIANDFNAKFVYIDTFNTDKSIVEDQFLNLLKEYEIDLVVLAKYMQILSDS
EEECCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIEQCTVNV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHEEEHHHHHHHHCCCCCCCCHHEEECC
SHRDEVDDLIRKGRDIERIALARAVRLHLNHQVFVYNSKTAVFD
CCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCEECCC
>Mature Secondary Structure
MEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWNSKNASL
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCC
NRYEIYKEFEKIADEVNGKFNVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGELKMKVPL
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEE
IISNHSDLENIANDFNAKFVYIDTFNTDKSIVEDQFLNLLKEYEIDLVVLAKYMQILSDS
EEECCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIEQCTVNV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHEEEHHHHHHHHCCCCCCCCHHEEECC
SHRDEVDDLIRKGRDIERIALARAVRLHLNHQVFVYNSKTAVFD
CCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]